Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03545 and RPSI07_RS03550 are separated by 150 nucleotides RPSI07_RS03550 and RPSI07_RS03555 are separated by 138 nucleotides RPSI07_RS03555 and RPSI07_RS03560 are separated by 66 nucleotides
RPSI07_RS03545: RPSI07_RS03545 - leucine-rich repeat domain-containing protein, at 962,822 to 964,768
_RS03545
RPSI07_RS03550: RPSI07_RS03550 - transcriptional regulator, at 964,919 to 965,320
_RS03550
RPSI07_RS03555: RPSI07_RS03555 - NAD(P)-dependent oxidoreductase, at 965,459 to 966,325
_RS03555
RPSI07_RS03560: RPSI07_RS03560 - hypothetical protein, at 966,392 to 970,312
_RS03560
Position (kb) 965
966
967 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 964.522 kb on - strand, within RPSI07_RS03545 at 964.661 kb on + strand at 964.662 kb on - strand at 964.662 kb on - strand at 964.662 kb on - strand at 964.662 kb on - strand at 964.662 kb on - strand at 964.662 kb on - strand at 964.920 kb on + strand at 964.920 kb on + strand at 964.920 kb on + strand at 964.920 kb on + strand at 964.920 kb on + strand at 964.920 kb on + strand at 964.920 kb on + strand at 964.921 kb on - strand at 964.921 kb on - strand at 964.921 kb on - strand at 964.921 kb on - strand at 964.921 kb on - strand at 964.944 kb on + strand at 965.046 kb on + strand, within RPSI07_RS03550 at 965.047 kb on - strand, within RPSI07_RS03550 at 965.073 kb on + strand, within RPSI07_RS03550 at 965.318 kb on + strand at 965.319 kb on - strand at 965.319 kb on - strand at 965.341 kb on + strand at 965.342 kb on - strand at 965.342 kb on - strand at 965.342 kb on - strand at 965.414 kb on + strand at 965.414 kb on + strand at 965.415 kb on - strand at 965.452 kb on + strand at 965.453 kb on - strand at 965.606 kb on + strand, within RPSI07_RS03555 at 965.606 kb on + strand, within RPSI07_RS03555 at 965.607 kb on - strand, within RPSI07_RS03555 at 965.637 kb on - strand, within RPSI07_RS03555 at 965.811 kb on - strand, within RPSI07_RS03555 at 965.811 kb on - strand, within RPSI07_RS03555 at 965.873 kb on + strand, within RPSI07_RS03555 at 965.873 kb on + strand, within RPSI07_RS03555 at 965.873 kb on + strand, within RPSI07_RS03555 at 965.874 kb on - strand, within RPSI07_RS03555 at 965.874 kb on - strand, within RPSI07_RS03555 at 965.874 kb on - strand, within RPSI07_RS03555 at 965.874 kb on - strand, within RPSI07_RS03555 at 965.874 kb on - strand, within RPSI07_RS03555 at 965.876 kb on + strand, within RPSI07_RS03555 at 965.876 kb on + strand, within RPSI07_RS03555 at 965.876 kb on + strand, within RPSI07_RS03555 at 965.877 kb on - strand, within RPSI07_RS03555 at 966.029 kb on + strand, within RPSI07_RS03555 at 966.030 kb on - strand, within RPSI07_RS03555 at 966.030 kb on - strand, within RPSI07_RS03555 at 966.780 kb on + strand at 966.780 kb on + strand at 966.780 kb on + strand at 966.781 kb on - strand at 966.781 kb on - strand at 966.781 kb on - strand at 966.781 kb on - strand at 966.978 kb on + strand, within RPSI07_RS03560 at 966.978 kb on + strand, within RPSI07_RS03560 at 966.978 kb on + strand, within RPSI07_RS03560 at 966.978 kb on + strand, within RPSI07_RS03560 at 966.978 kb on + strand, within RPSI07_RS03560 at 966.979 kb on - strand, within RPSI07_RS03560 at 966.981 kb on + strand, within RPSI07_RS03560 at 966.982 kb on - strand, within RPSI07_RS03560 at 967.101 kb on + strand, within RPSI07_RS03560 at 967.242 kb on + strand, within RPSI07_RS03560 at 967.242 kb on + strand, within RPSI07_RS03560 at 967.242 kb on + strand, within RPSI07_RS03560 at 967.242 kb on + strand, within RPSI07_RS03560 at 967.243 kb on - strand at 967.243 kb on - strand, within RPSI07_RS03560 at 967.243 kb on - strand, within RPSI07_RS03560
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 964,522 - RPSI07_RS03545 0.87 -1.3 964,661 + +0.3 964,662 - +3.1 964,662 - +1.0 964,662 - +0.2 964,662 - -0.2 964,662 - -0.9 964,662 - +3.1 964,920 + -0.8 964,920 + -0.4 964,920 + +0.5 964,920 + +0.6 964,920 + +1.7 964,920 + -1.1 964,920 + +0.0 964,921 - -0.2 964,921 - -0.4 964,921 - -0.8 964,921 - +0.3 964,921 - -1.5 964,944 + -0.2 965,046 + RPSI07_RS03550 0.32 -1.4 965,047 - RPSI07_RS03550 0.32 +0.3 965,073 + RPSI07_RS03550 0.38 -0.2 965,318 + +0.8 965,319 - -1.7 965,319 - -0.1 965,341 + -1.0 965,342 - -0.3 965,342 - +1.0 965,342 - +1.8 965,414 + +0.3 965,414 + -0.4 965,415 - -0.0 965,452 + -0.5 965,453 - -2.4 965,606 + RPSI07_RS03555 0.17 +0.6 965,606 + RPSI07_RS03555 0.17 -0.1 965,607 - RPSI07_RS03555 0.17 -0.2 965,637 - RPSI07_RS03555 0.21 -0.4 965,811 - RPSI07_RS03555 0.41 +3.1 965,811 - RPSI07_RS03555 0.41 +0.9 965,873 + RPSI07_RS03555 0.48 +1.8 965,873 + RPSI07_RS03555 0.48 -1.7 965,873 + RPSI07_RS03555 0.48 +1.8 965,874 - RPSI07_RS03555 0.48 -0.2 965,874 - RPSI07_RS03555 0.48 -1.0 965,874 - RPSI07_RS03555 0.48 -0.6 965,874 - RPSI07_RS03555 0.48 +0.0 965,874 - RPSI07_RS03555 0.48 -0.8 965,876 + RPSI07_RS03555 0.48 -1.3 965,876 + RPSI07_RS03555 0.48 -1.2 965,876 + RPSI07_RS03555 0.48 +0.8 965,877 - RPSI07_RS03555 0.48 -1.4 966,029 + RPSI07_RS03555 0.66 -1.6 966,030 - RPSI07_RS03555 0.66 -0.3 966,030 - RPSI07_RS03555 0.66 -2.9 966,780 + -1.4 966,780 + -0.3 966,780 + -0.2 966,781 - -1.5 966,781 - -0.6 966,781 - -0.2 966,781 - +0.9 966,978 + RPSI07_RS03560 0.15 -0.4 966,978 + RPSI07_RS03560 0.15 -1.6 966,978 + RPSI07_RS03560 0.15 +0.3 966,978 + RPSI07_RS03560 0.15 +2.8 966,978 + RPSI07_RS03560 0.15 +0.2 966,979 - RPSI07_RS03560 0.15 -0.1 966,981 + RPSI07_RS03560 0.15 -0.8 966,982 - RPSI07_RS03560 0.15 -0.8 967,101 + RPSI07_RS03560 0.18 +0.3 967,242 + RPSI07_RS03560 0.22 +0.2 967,242 + RPSI07_RS03560 0.22 -0.8 967,242 + RPSI07_RS03560 0.22 +0.5 967,242 + RPSI07_RS03560 0.22 +0.3 967,243 - +0.8 967,243 - RPSI07_RS03560 0.22 -1.1 967,243 - RPSI07_RS03560 0.22 +1.4
Or see this region's nucleotide sequence