Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS25270

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22660 and RPSI07_RS22665 are separated by 84 nucleotidesRPSI07_RS22665 and RPSI07_RS25270 are separated by 4 nucleotidesRPSI07_RS25270 and RPSI07_RS22670 are separated by 1 nucleotidesRPSI07_RS22670 and RPSI07_RS22675 are separated by 24 nucleotides RPSI07_RS22660: RPSI07_RS22660 - branched-chain amino acid ABC transporter substrate-binding protein, at 3,236,062 to 3,237,246 _RS22660 RPSI07_RS22665: RPSI07_RS22665 - hypothetical protein, at 3,237,331 to 3,237,531 _RS22665 RPSI07_RS25270: RPSI07_RS25270 - hypothetical protein, at 3,237,536 to 3,237,775 _RS25270 RPSI07_RS22670: RPSI07_RS22670 - IclR family transcriptional regulator, at 3,237,777 to 3,238,670 _RS22670 RPSI07_RS22675: RPSI07_RS22675 - FAA hydrolase family protein, at 3,238,695 to 3,239,687 _RS22675 Position (kb) 3237 3238Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3236.645 kb on + strand, within RPSI07_RS22660at 3236.645 kb on + strand, within RPSI07_RS22660at 3236.645 kb on + strand, within RPSI07_RS22660at 3236.645 kb on + strand, within RPSI07_RS22660at 3236.645 kb on + strand, within RPSI07_RS22660at 3236.646 kb on - strand, within RPSI07_RS22660at 3236.646 kb on - strand, within RPSI07_RS22660at 3236.646 kb on - strand, within RPSI07_RS22660at 3236.739 kb on + strand, within RPSI07_RS22660at 3236.741 kb on + strand, within RPSI07_RS22660at 3236.742 kb on - strand, within RPSI07_RS22660at 3236.742 kb on - strand, within RPSI07_RS22660at 3236.742 kb on - strand, within RPSI07_RS22660at 3237.539 kb on + strandat 3237.575 kb on + strand, within RPSI07_RS25270at 3237.575 kb on + strand, within RPSI07_RS25270at 3237.575 kb on + strand, within RPSI07_RS25270at 3237.575 kb on + strand, within RPSI07_RS25270at 3237.575 kb on + strand, within RPSI07_RS25270at 3237.576 kb on - strand, within RPSI07_RS25270at 3237.576 kb on - strand, within RPSI07_RS25270at 3237.576 kb on - strand, within RPSI07_RS25270at 3237.576 kb on - strand, within RPSI07_RS25270at 3237.576 kb on - strand, within RPSI07_RS25270at 3237.576 kb on - strand, within RPSI07_RS25270at 3237.577 kb on - strand, within RPSI07_RS25270at 3237.577 kb on - strand, within RPSI07_RS25270at 3237.616 kb on - strandat 3237.648 kb on + strand, within RPSI07_RS25270at 3237.739 kb on + strand, within RPSI07_RS25270at 3237.740 kb on - strand, within RPSI07_RS25270at 3237.747 kb on - strand, within RPSI07_RS25270at 3237.751 kb on + strand, within RPSI07_RS25270at 3237.751 kb on + strand, within RPSI07_RS25270at 3237.751 kb on + strand, within RPSI07_RS25270at 3237.751 kb on + strand, within RPSI07_RS25270at 3237.751 kb on + strand, within RPSI07_RS25270at 3237.752 kb on - strandat 3237.752 kb on - strandat 3237.969 kb on + strand, within RPSI07_RS22670at 3237.969 kb on + strand, within RPSI07_RS22670at 3237.982 kb on + strand, within RPSI07_RS22670at 3238.593 kb on + strandat 3238.668 kb on + strandat 3238.669 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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3,236,645 + RPSI07_RS22660 0.49 -2.2
3,236,645 + RPSI07_RS22660 0.49 -2.3
3,236,645 + RPSI07_RS22660 0.49 -1.4
3,236,645 + RPSI07_RS22660 0.49 +1.4
3,236,645 + RPSI07_RS22660 0.49 +0.8
3,236,646 - RPSI07_RS22660 0.49 +0.9
3,236,646 - RPSI07_RS22660 0.49 +0.3
3,236,646 - RPSI07_RS22660 0.49 +1.7
3,236,739 + RPSI07_RS22660 0.57 -1.3
3,236,741 + RPSI07_RS22660 0.57 -0.5
3,236,742 - RPSI07_RS22660 0.57 +0.2
3,236,742 - RPSI07_RS22660 0.57 +0.1
3,236,742 - RPSI07_RS22660 0.57 +1.0
3,237,539 + -1.0
3,237,575 + RPSI07_RS25270 0.16 -0.1
3,237,575 + RPSI07_RS25270 0.16 +0.3
3,237,575 + RPSI07_RS25270 0.16 -0.1
3,237,575 + RPSI07_RS25270 0.16 -2.2
3,237,575 + RPSI07_RS25270 0.16 +0.9
3,237,576 - RPSI07_RS25270 0.17 +0.9
3,237,576 - RPSI07_RS25270 0.17 +0.9
3,237,576 - RPSI07_RS25270 0.17 +1.3
3,237,576 - RPSI07_RS25270 0.17 +0.9
3,237,576 - RPSI07_RS25270 0.17 +0.3
3,237,576 - RPSI07_RS25270 0.17 -1.0
3,237,577 - RPSI07_RS25270 0.17 -1.0
3,237,577 - RPSI07_RS25270 0.17 -1.7
3,237,616 - -1.1
3,237,648 + RPSI07_RS25270 0.47 +0.3
3,237,739 + RPSI07_RS25270 0.85 +3.1
3,237,740 - RPSI07_RS25270 0.85 +0.8
3,237,747 - RPSI07_RS25270 0.88 +0.2
3,237,751 + RPSI07_RS25270 0.90 -0.3
3,237,751 + RPSI07_RS25270 0.90 +1.5
3,237,751 + RPSI07_RS25270 0.90 +1.9
3,237,751 + RPSI07_RS25270 0.90 +0.0
3,237,751 + RPSI07_RS25270 0.90 -1.2
3,237,752 - -0.5
3,237,752 - +0.7
3,237,969 + RPSI07_RS22670 0.21 +1.5
3,237,969 + RPSI07_RS22670 0.21 -0.7
3,237,982 + RPSI07_RS22670 0.23 +2.4
3,238,593 + +0.9
3,238,668 + -0.1
3,238,669 - +1.9

Or see this region's nucleotide sequence