Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS21420

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS21415 and RPSI07_RS21420 are separated by 154 nucleotidesRPSI07_RS21420 and RPSI07_RS21425 overlap by 4 nucleotidesRPSI07_RS21425 and RPSI07_RS21430 are separated by 62 nucleotides RPSI07_RS21415: RPSI07_RS21415 - apolipoprotein N-acyltransferase, at 2,966,874 to 2,968,385 _RS21415 RPSI07_RS21420: RPSI07_RS21420 - glycine--tRNA ligase subunit alpha, at 2,968,540 to 2,969,517 _RS21420 RPSI07_RS21425: RPSI07_RS21425 - hypothetical protein, at 2,969,514 to 2,970,167 _RS21425 RPSI07_RS21430: RPSI07_RS21430 - glycine--tRNA ligase subunit beta, at 2,970,230 to 2,972,323 _RS21430 Position (kb) 2968 2969 2970Strain fitness (log2 ratio) -1 0 1 2at 2969.633 kb on + strand, within RPSI07_RS21425at 2970.224 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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2,969,633 + RPSI07_RS21425 0.18 +1.9
2,970,224 + -0.7

Or see this region's nucleotide sequence