Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS20155 and RPSI07_RS20160 are separated by 22 nucleotides RPSI07_RS20160 and RPSI07_RS20165 are separated by 210 nucleotides RPSI07_RS20165 and RPSI07_RS20170 are separated by 9 nucleotides
RPSI07_RS20155: RPSI07_RS20155 - DUF72 domain-containing protein, at 2,709,450 to 2,710,517
_RS20155
RPSI07_RS20160: RPSI07_RS20160 - peptide-methionine (S)-S-oxide reductase, at 2,710,540 to 2,711,100
_RS20160
RPSI07_RS20165: RPSI07_RS20165 - flavin reductase, at 2,711,311 to 2,711,877
_RS20165
RPSI07_RS20170: RPSI07_RS20170 - DUF3079 domain-containing protein, at 2,711,887 to 2,712,093
_RS20170
Position (kb)
2710
2711
2712 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2709.663 kb on + strand, within RPSI07_RS20155 at 2709.714 kb on + strand, within RPSI07_RS20155 at 2709.762 kb on + strand, within RPSI07_RS20155 at 2709.763 kb on - strand, within RPSI07_RS20155 at 2709.786 kb on + strand, within RPSI07_RS20155 at 2709.786 kb on + strand, within RPSI07_RS20155 at 2709.786 kb on + strand, within RPSI07_RS20155 at 2709.787 kb on - strand, within RPSI07_RS20155 at 2709.787 kb on - strand, within RPSI07_RS20155 at 2710.323 kb on + strand, within RPSI07_RS20155 at 2710.324 kb on - strand, within RPSI07_RS20155 at 2710.610 kb on + strand, within RPSI07_RS20160 at 2710.962 kb on - strand, within RPSI07_RS20160 at 2710.991 kb on + strand, within RPSI07_RS20160 at 2711.156 kb on + strand at 2711.157 kb on - strand at 2711.257 kb on - strand at 2711.325 kb on + strand at 2711.590 kb on + strand, within RPSI07_RS20165 at 2711.590 kb on + strand, within RPSI07_RS20165 at 2711.590 kb on + strand, within RPSI07_RS20165 at 2711.590 kb on + strand, within RPSI07_RS20165 at 2711.753 kb on - strand, within RPSI07_RS20165 at 2711.753 kb on - strand, within RPSI07_RS20165 at 2711.948 kb on + strand, within RPSI07_RS20170 at 2711.949 kb on - strand, within RPSI07_RS20170 at 2712.029 kb on + strand, within RPSI07_RS20170 at 2712.029 kb on + strand, within RPSI07_RS20170 at 2712.029 kb on + strand, within RPSI07_RS20170 at 2712.030 kb on - strand, within RPSI07_RS20170 at 2712.030 kb on - strand, within RPSI07_RS20170 at 2712.030 kb on - strand, within RPSI07_RS20170 at 2712.030 kb on - strand, within RPSI07_RS20170 at 2712.030 kb on - strand, within RPSI07_RS20170
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 2,709,663 + RPSI07_RS20155 0.20 -1.4 2,709,714 + RPSI07_RS20155 0.25 +1.8 2,709,762 + RPSI07_RS20155 0.29 -2.2 2,709,763 - RPSI07_RS20155 0.29 +1.8 2,709,786 + RPSI07_RS20155 0.31 +0.4 2,709,786 + RPSI07_RS20155 0.31 -0.5 2,709,786 + RPSI07_RS20155 0.31 -0.6 2,709,787 - RPSI07_RS20155 0.32 -0.8 2,709,787 - RPSI07_RS20155 0.32 +0.8 2,710,323 + RPSI07_RS20155 0.82 -0.3 2,710,324 - RPSI07_RS20155 0.82 -0.7 2,710,610 + RPSI07_RS20160 0.12 +0.5 2,710,962 - RPSI07_RS20160 0.75 +1.4 2,710,991 + RPSI07_RS20160 0.80 -2.4 2,711,156 + -0.2 2,711,157 - -0.6 2,711,257 - -0.8 2,711,325 + +2.8 2,711,590 + RPSI07_RS20165 0.49 -0.4 2,711,590 + RPSI07_RS20165 0.49 +1.8 2,711,590 + RPSI07_RS20165 0.49 -0.2 2,711,590 + RPSI07_RS20165 0.49 -1.6 2,711,753 - RPSI07_RS20165 0.78 +0.8 2,711,753 - RPSI07_RS20165 0.78 -0.7 2,711,948 + RPSI07_RS20170 0.29 -1.3 2,711,949 - RPSI07_RS20170 0.30 +0.5 2,712,029 + RPSI07_RS20170 0.69 -1.0 2,712,029 + RPSI07_RS20170 0.69 -0.1 2,712,029 + RPSI07_RS20170 0.69 +0.6 2,712,030 - RPSI07_RS20170 0.69 +1.4 2,712,030 - RPSI07_RS20170 0.69 +1.7 2,712,030 - RPSI07_RS20170 0.69 -0.2 2,712,030 - RPSI07_RS20170 0.69 +0.7 2,712,030 - RPSI07_RS20170 0.69 -0.3
Or see this region's nucleotide sequence