Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS18465

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS25170 and RPSI07_RS18460 are separated by 135 nucleotidesRPSI07_RS18460 and RPSI07_RS18465 are separated by 34 nucleotidesRPSI07_RS18465 and RPSI07_RS18470 are separated by 39 nucleotides RPSI07_RS25170: RPSI07_RS25170 - hypothetical protein, at 2,344,067 to 2,344,339 _RS25170 RPSI07_RS18460: RPSI07_RS18460 - hypothetical protein, at 2,344,475 to 2,345,260 _RS18460 RPSI07_RS18465: RPSI07_RS18465 - outer membrane protein assembly factor BamC, at 2,345,295 to 2,346,491 _RS18465 RPSI07_RS18470: RPSI07_RS18470 - 4-hydroxy-tetrahydrodipicolinate synthase, at 2,346,531 to 2,347,415 _RS18470 Position (kb) 2345 2346 2347Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2344.337 kb on + strandat 2344.455 kb on - strandat 2344.716 kb on + strand, within RPSI07_RS18460at 2344.716 kb on + strand, within RPSI07_RS18460at 2344.897 kb on - strand, within RPSI07_RS18460at 2346.860 kb on - strand, within RPSI07_RS18470at 2346.860 kb on - strand, within RPSI07_RS18470at 2346.984 kb on - strand, within RPSI07_RS18470at 2347.064 kb on - strand, within RPSI07_RS18470at 2347.446 kb on - strandat 2347.461 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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2,344,337 + +4.6
2,344,455 - +0.1
2,344,716 + RPSI07_RS18460 0.31 -0.2
2,344,716 + RPSI07_RS18460 0.31 -0.0
2,344,897 - RPSI07_RS18460 0.54 -0.7
2,346,860 - RPSI07_RS18470 0.37 -0.8
2,346,860 - RPSI07_RS18470 0.37 -1.0
2,346,984 - RPSI07_RS18470 0.51 -1.5
2,347,064 - RPSI07_RS18470 0.60 -1.8
2,347,446 - -0.7
2,347,461 + -0.5

Or see this region's nucleotide sequence