Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS14885 and RPSI07_RS14890 are separated by 83 nucleotides RPSI07_RS14890 and RPSI07_RS25065 are separated by 271 nucleotides RPSI07_RS25065 and RPSI07_RS14900 are separated by 163 nucleotides
RPSI07_RS14885: RPSI07_RS14885 - cation acetate symporter, at 1,509,949 to 1,512,015
_RS14885
RPSI07_RS14890: RPSI07_RS14890 - tRNA-Ser, at 1,512,099 to 1,512,189
_RS14890
RPSI07_RS25065: RPSI07_RS25065 - XRE family transcriptional regulator, at 1,512,461 to 1,512,742
_RS25065
RPSI07_RS14900: RPSI07_RS14900 - IS3 family transposase, at 1,512,906 to 1,514,047
_RS14900
Position (kb)
1512
1513 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1511.127 kb on + strand, within RPSI07_RS14885 at 1511.163 kb on + strand, within RPSI07_RS14885 at 1511.164 kb on - strand, within RPSI07_RS14885 at 1511.230 kb on + strand, within RPSI07_RS14885 at 1511.230 kb on + strand, within RPSI07_RS14885 at 1511.231 kb on - strand, within RPSI07_RS14885 at 1511.231 kb on - strand, within RPSI07_RS14885 at 1511.231 kb on - strand, within RPSI07_RS14885 at 1511.231 kb on - strand, within RPSI07_RS14885 at 1511.231 kb on - strand, within RPSI07_RS14885 at 1511.458 kb on + strand, within RPSI07_RS14885 at 1511.459 kb on - strand, within RPSI07_RS14885 at 1511.459 kb on - strand, within RPSI07_RS14885 at 1511.560 kb on + strand, within RPSI07_RS14885 at 1511.561 kb on - strand, within RPSI07_RS14885 at 1511.561 kb on - strand, within RPSI07_RS14885 at 1511.561 kb on - strand, within RPSI07_RS14885 at 1511.650 kb on + strand, within RPSI07_RS14885 at 1511.651 kb on - strand, within RPSI07_RS14885 at 1511.651 kb on - strand, within RPSI07_RS14885 at 1511.651 kb on - strand, within RPSI07_RS14885 at 1511.818 kb on + strand at 1511.818 kb on + strand at 1511.818 kb on + strand at 1511.818 kb on + strand at 1511.818 kb on + strand at 1511.818 kb on + strand at 1511.818 kb on + strand at 1511.819 kb on - strand at 1511.819 kb on - strand at 1511.819 kb on - strand at 1511.819 kb on - strand at 1511.819 kb on - strand at 1511.833 kb on + strand at 1511.834 kb on - strand at 1511.834 kb on - strand at 1511.995 kb on + strand at 1511.996 kb on - strand at 1512.014 kb on - strand at 1512.014 kb on - strand at 1512.041 kb on - strand at 1512.197 kb on - strand at 1512.197 kb on - strand at 1512.199 kb on - strand at 1512.317 kb on + strand at 1512.318 kb on - strand at 1512.438 kb on + strand at 1512.438 kb on + strand at 1512.439 kb on - strand at 1512.439 kb on - strand at 1512.439 kb on - strand at 1512.469 kb on + strand at 1512.469 kb on + strand at 1512.469 kb on + strand at 1512.470 kb on - strand at 1512.470 kb on - strand at 1512.470 kb on - strand at 1512.470 kb on - strand at 1512.470 kb on - strand at 1512.472 kb on + strand at 1512.473 kb on - strand at 1512.597 kb on + strand, within RPSI07_RS25065 at 1512.597 kb on + strand, within RPSI07_RS25065 at 1512.597 kb on + strand, within RPSI07_RS25065 at 1512.597 kb on + strand, within RPSI07_RS25065 at 1512.597 kb on + strand, within RPSI07_RS25065 at 1512.597 kb on + strand, within RPSI07_RS25065 at 1512.598 kb on - strand, within RPSI07_RS25065 at 1512.598 kb on - strand, within RPSI07_RS25065 at 1512.598 kb on - strand, within RPSI07_RS25065 at 1512.598 kb on - strand, within RPSI07_RS25065 at 1512.598 kb on - strand, within RPSI07_RS25065 at 1512.598 kb on - strand, within RPSI07_RS25065 at 1512.599 kb on - strand, within RPSI07_RS25065 at 1512.718 kb on + strand at 1512.718 kb on + strand at 1512.718 kb on + strand at 1512.719 kb on - strand at 1512.719 kb on - strand at 1512.719 kb on - strand at 1512.754 kb on - strand at 1512.823 kb on + strand at 1512.823 kb on + strand at 1512.824 kb on - strand at 1512.824 kb on - strand at 1512.824 kb on - strand at 1512.824 kb on - strand at 1512.824 kb on - strand at 1512.824 kb on - strand at 1512.861 kb on - strand at 1512.861 kb on - strand at 1512.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,511,127 + RPSI07_RS14885 0.57 -0.7 1,511,163 + RPSI07_RS14885 0.59 +0.2 1,511,164 - RPSI07_RS14885 0.59 -1.2 1,511,230 + RPSI07_RS14885 0.62 +0.8 1,511,230 + RPSI07_RS14885 0.62 +0.8 1,511,231 - RPSI07_RS14885 0.62 -0.2 1,511,231 - RPSI07_RS14885 0.62 +2.4 1,511,231 - RPSI07_RS14885 0.62 -0.1 1,511,231 - RPSI07_RS14885 0.62 +0.6 1,511,231 - RPSI07_RS14885 0.62 -0.2 1,511,458 + RPSI07_RS14885 0.73 -0.4 1,511,459 - RPSI07_RS14885 0.73 -3.4 1,511,459 - RPSI07_RS14885 0.73 +0.8 1,511,560 + RPSI07_RS14885 0.78 +0.7 1,511,561 - RPSI07_RS14885 0.78 -0.0 1,511,561 - RPSI07_RS14885 0.78 -0.1 1,511,561 - RPSI07_RS14885 0.78 +0.1 1,511,650 + RPSI07_RS14885 0.82 +1.6 1,511,651 - RPSI07_RS14885 0.82 -0.5 1,511,651 - RPSI07_RS14885 0.82 +0.4 1,511,651 - RPSI07_RS14885 0.82 +0.4 1,511,818 + +0.4 1,511,818 + -0.4 1,511,818 + +0.8 1,511,818 + +0.2 1,511,818 + +0.7 1,511,818 + +0.3 1,511,818 + -1.0 1,511,819 - -0.8 1,511,819 - -0.6 1,511,819 - -1.8 1,511,819 - +0.3 1,511,819 - -0.5 1,511,833 + +1.8 1,511,834 - +0.6 1,511,834 - +1.9 1,511,995 + -0.3 1,511,996 - -0.2 1,512,014 - +1.8 1,512,014 - +2.2 1,512,041 - +0.4 1,512,197 - -1.0 1,512,197 - +0.4 1,512,199 - +1.8 1,512,317 + +1.8 1,512,318 - -0.9 1,512,438 + +0.2 1,512,438 + +0.8 1,512,439 - -0.4 1,512,439 - -0.7 1,512,439 - -0.2 1,512,469 + +0.8 1,512,469 + -1.2 1,512,469 + -0.8 1,512,470 - -0.6 1,512,470 - +0.9 1,512,470 - -0.4 1,512,470 - +1.1 1,512,470 - +0.6 1,512,472 + +1.2 1,512,473 - -0.5 1,512,597 + RPSI07_RS25065 0.48 -0.2 1,512,597 + RPSI07_RS25065 0.48 -1.2 1,512,597 + RPSI07_RS25065 0.48 -1.6 1,512,597 + RPSI07_RS25065 0.48 -0.2 1,512,597 + RPSI07_RS25065 0.48 -2.5 1,512,597 + RPSI07_RS25065 0.48 +0.1 1,512,598 - RPSI07_RS25065 0.49 +2.8 1,512,598 - RPSI07_RS25065 0.49 -2.3 1,512,598 - RPSI07_RS25065 0.49 -0.3 1,512,598 - RPSI07_RS25065 0.49 +1.1 1,512,598 - RPSI07_RS25065 0.49 -0.4 1,512,598 - RPSI07_RS25065 0.49 -1.8 1,512,599 - RPSI07_RS25065 0.49 +0.3 1,512,718 + +1.0 1,512,718 + -1.0 1,512,718 + +2.2 1,512,719 - +2.8 1,512,719 - +1.8 1,512,719 - -0.2 1,512,754 - -0.0 1,512,823 + -1.1 1,512,823 + +0.4 1,512,824 - +1.4 1,512,824 - -0.8 1,512,824 - +0.8 1,512,824 - +1.7 1,512,824 - -1.2 1,512,824 - -0.1 1,512,861 - -0.3 1,512,861 - -0.2 1,512,861 - -0.2
Or see this region's nucleotide sequence