Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS14730

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS14720 and RPSI07_RS14725 overlap by 4 nucleotidesRPSI07_RS14725 and RPSI07_RS14730 are separated by 94 nucleotidesRPSI07_RS14730 and RPSI07_RS14735 are separated by 4 nucleotidesRPSI07_RS14735 and RPSI07_RS14740 are separated by 14 nucleotides RPSI07_RS14720: RPSI07_RS14720 - tRNA pseudouridine(38-40) synthase TruA, at 1,479,046 to 1,479,870 _RS14720 RPSI07_RS14725: RPSI07_RS14725 - phosphoribosylanthranilate isomerase, at 1,479,867 to 1,480,571 _RS14725 RPSI07_RS14730: RPSI07_RS14730 - tryptophan synthase subunit beta, at 1,480,666 to 1,481,859 _RS14730 RPSI07_RS14735: RPSI07_RS14735 - site-specific DNA-methyltransferase, at 1,481,864 to 1,482,676 _RS14735 RPSI07_RS14740: RPSI07_RS14740 - tryptophan synthase subunit alpha, at 1,482,691 to 1,483,488 _RS14740 Position (kb) 1480 1481 1482Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1479.714 kb on - strand, within RPSI07_RS14720at 1480.670 kb on - strandat 1480.804 kb on + strand, within RPSI07_RS14730at 1480.805 kb on - strand, within RPSI07_RS14730at 1480.850 kb on - strand, within RPSI07_RS14730at 1480.894 kb on + strand, within RPSI07_RS14730at 1480.894 kb on + strand, within RPSI07_RS14730at 1480.895 kb on - strand, within RPSI07_RS14730at 1481.072 kb on - strand, within RPSI07_RS14730at 1481.399 kb on - strand, within RPSI07_RS14730at 1481.404 kb on + strand, within RPSI07_RS14730at 1481.645 kb on - strand, within RPSI07_RS14730at 1481.984 kb on + strand, within RPSI07_RS14735at 1481.984 kb on + strand, within RPSI07_RS14735at 1482.021 kb on - strand, within RPSI07_RS14735at 1482.021 kb on - strand, within RPSI07_RS14735at 1482.086 kb on + strand, within RPSI07_RS14735at 1482.257 kb on + strand, within RPSI07_RS14735at 1482.257 kb on + strand, within RPSI07_RS14735at 1482.690 kb on + strandat 1482.690 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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1,479,714 - RPSI07_RS14720 0.81 +0.1
1,480,670 - -0.2
1,480,804 + RPSI07_RS14730 0.12 -0.2
1,480,805 - RPSI07_RS14730 0.12 +0.8
1,480,850 - RPSI07_RS14730 0.15 +0.8
1,480,894 + RPSI07_RS14730 0.19 -0.2
1,480,894 + RPSI07_RS14730 0.19 +0.8
1,480,895 - RPSI07_RS14730 0.19 -0.8
1,481,072 - RPSI07_RS14730 0.34 +0.8
1,481,399 - RPSI07_RS14730 0.61 +0.8
1,481,404 + RPSI07_RS14730 0.62 +0.8
1,481,645 - RPSI07_RS14730 0.82 -1.5
1,481,984 + RPSI07_RS14735 0.15 -4.8
1,481,984 + RPSI07_RS14735 0.15 -1.5
1,482,021 - RPSI07_RS14735 0.19 -2.2
1,482,021 - RPSI07_RS14735 0.19 +1.8
1,482,086 + RPSI07_RS14735 0.27 +0.2
1,482,257 + RPSI07_RS14735 0.48 -0.3
1,482,257 + RPSI07_RS14735 0.48 -4.9
1,482,690 + +0.8
1,482,690 + +0.8

Or see this region's nucleotide sequence