Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13070 and RPSI07_RS13075 are separated by 3 nucleotides RPSI07_RS13075 and RPSI07_RS13080 are separated by 110 nucleotides RPSI07_RS13080 and RPSI07_RS13085 are separated by 108 nucleotides RPSI07_RS13085 and RPSI07_RS13090 overlap by 1 nucleotides
RPSI07_RS13070: RPSI07_RS13070 - hypothetical protein, at 1,119,835 to 1,120,110
_RS13070
RPSI07_RS13075: RPSI07_RS13075 - hypothetical protein, at 1,120,114 to 1,120,380
_RS13075
RPSI07_RS13080: RPSI07_RS13080 - argininosuccinate lyase, at 1,120,491 to 1,121,906
_RS13080
RPSI07_RS13085: RPSI07_RS13085 - membrane protein, at 1,122,015 to 1,122,734
_RS13085
RPSI07_RS13090: RPSI07_RS13090 - DNA-directed RNA polymerase sigma-70 factor, at 1,122,734 to 1,123,375
_RS13090
Position (kb)
1120
1121
1122 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1119.543 kb on + strand at 1119.543 kb on + strand at 1119.544 kb on - strand at 1119.549 kb on + strand at 1119.549 kb on + strand at 1119.643 kb on + strand at 1119.643 kb on + strand at 1119.643 kb on + strand at 1119.644 kb on - strand at 1119.747 kb on + strand at 1119.748 kb on - strand at 1119.748 kb on - strand at 1119.748 kb on - strand at 1119.856 kb on + strand at 1119.856 kb on + strand at 1119.893 kb on + strand, within RPSI07_RS13070 at 1119.894 kb on - strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1119.988 kb on + strand, within RPSI07_RS13070 at 1120.021 kb on + strand, within RPSI07_RS13070 at 1120.021 kb on + strand, within RPSI07_RS13070 at 1120.021 kb on + strand, within RPSI07_RS13070 at 1120.022 kb on - strand, within RPSI07_RS13070 at 1120.022 kb on - strand, within RPSI07_RS13070 at 1120.022 kb on - strand, within RPSI07_RS13070 at 1120.022 kb on - strand, within RPSI07_RS13070 at 1120.022 kb on - strand, within RPSI07_RS13070 at 1120.032 kb on + strand, within RPSI07_RS13070 at 1120.175 kb on - strand, within RPSI07_RS13075 at 1120.224 kb on + strand, within RPSI07_RS13075 at 1120.224 kb on + strand, within RPSI07_RS13075 at 1120.224 kb on + strand, within RPSI07_RS13075 at 1120.224 kb on + strand, within RPSI07_RS13075 at 1120.307 kb on - strand, within RPSI07_RS13075 at 1120.339 kb on + strand, within RPSI07_RS13075 at 1120.339 kb on + strand, within RPSI07_RS13075 at 1120.339 kb on + strand, within RPSI07_RS13075 at 1120.339 kb on + strand, within RPSI07_RS13075 at 1120.339 kb on + strand, within RPSI07_RS13075 at 1120.339 kb on + strand, within RPSI07_RS13075 at 1120.339 kb on + strand, within RPSI07_RS13075 at 1120.339 kb on + strand, within RPSI07_RS13075 at 1120.340 kb on - strand, within RPSI07_RS13075 at 1120.340 kb on - strand, within RPSI07_RS13075 at 1120.340 kb on - strand, within RPSI07_RS13075 at 1120.371 kb on + strand at 1120.371 kb on + strand at 1120.372 kb on - strand at 1120.422 kb on + strand at 1121.972 kb on + strand at 1121.972 kb on + strand at 1121.972 kb on + strand at 1121.973 kb on - strand at 1121.973 kb on - strand at 1122.145 kb on - strand, within RPSI07_RS13085 at 1122.586 kb on + strand, within RPSI07_RS13085 at 1122.685 kb on + strand at 1122.852 kb on + strand, within RPSI07_RS13090 at 1122.853 kb on - strand, within RPSI07_RS13090 at 1122.853 kb on - strand, within RPSI07_RS13090
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,119,543 + +0.2 1,119,543 + +0.7 1,119,544 - +0.4 1,119,549 + -0.9 1,119,549 + +1.3 1,119,643 + +1.3 1,119,643 + -1.5 1,119,643 + -0.0 1,119,644 - -1.6 1,119,747 + -1.3 1,119,748 - -0.8 1,119,748 - +0.5 1,119,748 - -0.7 1,119,856 + +1.4 1,119,856 + -0.2 1,119,893 + RPSI07_RS13070 0.21 +0.8 1,119,894 - RPSI07_RS13070 0.21 +0.3 1,119,988 + RPSI07_RS13070 0.55 -1.7 1,119,988 + RPSI07_RS13070 0.55 -1.1 1,119,988 + RPSI07_RS13070 0.55 +1.4 1,119,988 + RPSI07_RS13070 0.55 -0.3 1,119,988 + RPSI07_RS13070 0.55 -0.2 1,119,988 + RPSI07_RS13070 0.55 +0.4 1,119,988 + RPSI07_RS13070 0.55 -0.1 1,119,988 + RPSI07_RS13070 0.55 -1.1 1,119,988 + RPSI07_RS13070 0.55 -0.1 1,120,021 + RPSI07_RS13070 0.67 -0.8 1,120,021 + RPSI07_RS13070 0.67 +4.2 1,120,021 + RPSI07_RS13070 0.67 -0.9 1,120,022 - RPSI07_RS13070 0.68 -0.0 1,120,022 - RPSI07_RS13070 0.68 +1.7 1,120,022 - RPSI07_RS13070 0.68 +1.2 1,120,022 - RPSI07_RS13070 0.68 +0.3 1,120,022 - RPSI07_RS13070 0.68 -0.4 1,120,032 + RPSI07_RS13070 0.71 +0.7 1,120,175 - RPSI07_RS13075 0.23 -0.4 1,120,224 + RPSI07_RS13075 0.41 +0.2 1,120,224 + RPSI07_RS13075 0.41 +1.0 1,120,224 + RPSI07_RS13075 0.41 -0.1 1,120,224 + RPSI07_RS13075 0.41 +0.2 1,120,307 - RPSI07_RS13075 0.72 -0.4 1,120,339 + RPSI07_RS13075 0.84 +0.9 1,120,339 + RPSI07_RS13075 0.84 -1.6 1,120,339 + RPSI07_RS13075 0.84 +0.0 1,120,339 + RPSI07_RS13075 0.84 +5.0 1,120,339 + RPSI07_RS13075 0.84 +3.4 1,120,339 + RPSI07_RS13075 0.84 +0.2 1,120,339 + RPSI07_RS13075 0.84 -1.9 1,120,339 + RPSI07_RS13075 0.84 +1.9 1,120,340 - RPSI07_RS13075 0.85 +3.7 1,120,340 - RPSI07_RS13075 0.85 +0.2 1,120,340 - RPSI07_RS13075 0.85 -0.3 1,120,371 + -0.4 1,120,371 + -0.3 1,120,372 - +0.5 1,120,422 + +0.3 1,121,972 + -0.3 1,121,972 + -1.5 1,121,972 + +1.2 1,121,973 - -0.1 1,121,973 - -0.0 1,122,145 - RPSI07_RS13085 0.18 +0.5 1,122,586 + RPSI07_RS13085 0.79 +0.6 1,122,685 + -0.7 1,122,852 + RPSI07_RS13090 0.18 +0.0 1,122,853 - RPSI07_RS13090 0.19 -1.9 1,122,853 - RPSI07_RS13090 0.19 +0.2
Or see this region's nucleotide sequence