Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS08710 and RPSI07_RS08715 are separated by 275 nucleotides RPSI07_RS08715 and RPSI07_RS08720 overlap by 4 nucleotides RPSI07_RS08720 and RPSI07_RS08725 are separated by 202 nucleotides RPSI07_RS08725 and RPSI07_RS08730 overlap by 4 nucleotides
RPSI07_RS08710: RPSI07_RS08710 - hypothetical protein, at 190,246 to 190,515
_RS08710
RPSI07_RS08715: RPSI07_RS08715 - exonuclease, at 190,791 to 191,486
_RS08715
RPSI07_RS08720: RPSI07_RS08720 - MBL fold metallo-hydrolase, at 191,483 to 192,139
_RS08720
RPSI07_RS08725: RPSI07_RS08725 - septal ring lytic transglycosylase RlpA family protein, at 192,342 to 193,049
_RS08725
RPSI07_RS08730: RPSI07_RS08730 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 193,046 to 193,948
_RS08730
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 190.488 kb on + strand, within RPSI07_RS08710 at 190.488 kb on + strand, within RPSI07_RS08710 at 190.489 kb on - strand at 190.521 kb on + strand at 190.522 kb on - strand at 190.522 kb on - strand at 190.522 kb on - strand at 190.574 kb on + strand at 190.575 kb on - strand at 190.575 kb on - strand at 190.575 kb on - strand at 190.577 kb on - strand at 190.612 kb on + strand at 190.612 kb on + strand at 190.612 kb on + strand at 190.612 kb on + strand at 190.612 kb on + strand at 190.612 kb on + strand at 190.612 kb on + strand at 190.612 kb on + strand at 190.613 kb on - strand at 190.613 kb on - strand at 190.613 kb on - strand at 190.613 kb on - strand at 190.613 kb on - strand at 190.613 kb on - strand at 190.613 kb on - strand at 190.680 kb on + strand at 190.680 kb on + strand at 190.680 kb on + strand at 190.680 kb on + strand at 190.680 kb on + strand at 190.681 kb on - strand at 190.910 kb on + strand, within RPSI07_RS08715 at 190.910 kb on + strand, within RPSI07_RS08715 at 190.911 kb on - strand, within RPSI07_RS08715 at 191.056 kb on + strand, within RPSI07_RS08715 at 191.059 kb on + strand, within RPSI07_RS08715 at 191.060 kb on - strand, within RPSI07_RS08715 at 191.380 kb on + strand, within RPSI07_RS08715 at 191.381 kb on - strand, within RPSI07_RS08715 at 191.381 kb on - strand, within RPSI07_RS08715 at 191.381 kb on - strand, within RPSI07_RS08715 at 191.381 kb on - strand, within RPSI07_RS08715 at 191.381 kb on - strand, within RPSI07_RS08715 at 191.383 kb on - strand, within RPSI07_RS08715 at 191.523 kb on + strand at 191.523 kb on + strand at 191.524 kb on - strand at 191.524 kb on - strand at 191.524 kb on - strand at 192.115 kb on + strand at 192.116 kb on - strand at 192.307 kb on + strand at 192.328 kb on + strand at 192.330 kb on - strand at 192.790 kb on - strand, within RPSI07_RS08725 at 192.841 kb on - strand, within RPSI07_RS08725 at 192.841 kb on - strand, within RPSI07_RS08725 at 192.841 kb on - strand, within RPSI07_RS08725 at 193.048 kb on - strand at 193.086 kb on + strand at 193.087 kb on - strand at 193.087 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 190,488 + RPSI07_RS08710 0.90 -1.6 190,488 + RPSI07_RS08710 0.90 -0.3 190,489 - +0.5 190,521 + -0.4 190,522 - -0.1 190,522 - -0.7 190,522 - +1.0 190,574 + -0.4 190,575 - +1.0 190,575 - +1.0 190,575 - -0.0 190,577 - -1.6 190,612 + +0.3 190,612 + -1.1 190,612 + +2.0 190,612 + +1.6 190,612 + +1.2 190,612 + +1.8 190,612 + +0.3 190,612 + +0.4 190,613 - +0.1 190,613 - +0.7 190,613 - +2.0 190,613 - +0.3 190,613 - -0.0 190,613 - +0.6 190,613 - -1.8 190,680 + +1.8 190,680 + +0.2 190,680 + -0.9 190,680 + +0.2 190,680 + -2.7 190,681 - +1.4 190,910 + RPSI07_RS08715 0.17 +2.0 190,910 + RPSI07_RS08715 0.17 -2.4 190,911 - RPSI07_RS08715 0.17 -0.7 191,056 + RPSI07_RS08715 0.38 -0.6 191,059 + RPSI07_RS08715 0.39 -0.7 191,060 - RPSI07_RS08715 0.39 -1.3 191,380 + RPSI07_RS08715 0.85 -1.6 191,381 - RPSI07_RS08715 0.85 -2.2 191,381 - RPSI07_RS08715 0.85 +0.0 191,381 - RPSI07_RS08715 0.85 -0.7 191,381 - RPSI07_RS08715 0.85 +0.3 191,381 - RPSI07_RS08715 0.85 -1.0 191,383 - RPSI07_RS08715 0.85 +1.6 191,523 + +3.0 191,523 + +1.2 191,524 - +1.6 191,524 - -0.4 191,524 - -1.6 192,115 + -1.0 192,116 - -0.8 192,307 + +0.6 192,328 + +7.9 192,330 - -0.6 192,790 - RPSI07_RS08725 0.63 -2.0 192,841 - RPSI07_RS08725 0.70 +0.2 192,841 - RPSI07_RS08725 0.70 +1.0 192,841 - RPSI07_RS08725 0.70 -0.8 193,048 - -1.3 193,086 + -0.3 193,087 - -0.0 193,087 - +1.0
Or see this region's nucleotide sequence