Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS08400

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS08395 and RPSI07_RS08400 are separated by 108 nucleotidesRPSI07_RS08400 and RPSI07_RS08405 are separated by 305 nucleotides RPSI07_RS08395: RPSI07_RS08395 - LysR family transcriptional regulator, at 117,594 to 118,490 _RS08395 RPSI07_RS08400: RPSI07_RS08400 - short chain dehydrogenase, at 118,599 to 119,204 _RS08400 RPSI07_RS08405: RPSI07_RS08405 - branched-chain amino acid ABC transporter substrate-binding protein, at 119,510 to 120,658 _RS08405 Position (kb) 118 119 120Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 117.712 kb on + strand, within RPSI07_RS08395at 117.980 kb on - strand, within RPSI07_RS08395at 118.263 kb on + strand, within RPSI07_RS08395at 118.263 kb on + strand, within RPSI07_RS08395at 118.263 kb on + strand, within RPSI07_RS08395at 118.264 kb on - strand, within RPSI07_RS08395at 118.294 kb on + strand, within RPSI07_RS08395at 118.294 kb on + strand, within RPSI07_RS08395at 118.294 kb on + strand, within RPSI07_RS08395at 118.384 kb on + strand, within RPSI07_RS08395at 118.384 kb on + strand, within RPSI07_RS08395at 118.384 kb on + strand, within RPSI07_RS08395at 118.384 kb on + strand, within RPSI07_RS08395at 118.385 kb on - strand, within RPSI07_RS08395at 118.491 kb on - strandat 118.535 kb on - strandat 118.711 kb on - strand, within RPSI07_RS08400at 119.094 kb on + strand, within RPSI07_RS08400at 119.094 kb on + strand, within RPSI07_RS08400at 119.095 kb on - strand, within RPSI07_RS08400at 119.151 kb on + strandat 119.151 kb on + strandat 119.256 kb on - strandat 119.304 kb on + strandat 119.304 kb on + strandat 119.400 kb on + strandat 119.434 kb on + strandat 119.434 kb on + strandat 119.434 kb on + strandat 119.435 kb on - strandat 119.693 kb on + strand, within RPSI07_RS08405at 119.693 kb on + strand, within RPSI07_RS08405at 119.693 kb on + strand, within RPSI07_RS08405at 119.693 kb on + strand, within RPSI07_RS08405at 119.693 kb on + strand, within RPSI07_RS08405at 119.694 kb on - strand, within RPSI07_RS08405at 119.694 kb on - strand, within RPSI07_RS08405at 119.851 kb on + strand, within RPSI07_RS08405at 119.852 kb on - strand, within RPSI07_RS08405at 119.852 kb on - strand, within RPSI07_RS08405at 120.012 kb on - strand, within RPSI07_RS08405at 120.012 kb on - strand, within RPSI07_RS08405

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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117,712 + RPSI07_RS08395 0.13 -0.5
117,980 - RPSI07_RS08395 0.43 +0.1
118,263 + RPSI07_RS08395 0.75 +1.2
118,263 + RPSI07_RS08395 0.75 -0.1
118,263 + RPSI07_RS08395 0.75 -0.6
118,264 - RPSI07_RS08395 0.75 -0.2
118,294 + RPSI07_RS08395 0.78 +3.0
118,294 + RPSI07_RS08395 0.78 +1.0
118,294 + RPSI07_RS08395 0.78 +0.0
118,384 + RPSI07_RS08395 0.88 -1.3
118,384 + RPSI07_RS08395 0.88 -1.3
118,384 + RPSI07_RS08395 0.88 -1.3
118,384 + RPSI07_RS08395 0.88 -1.2
118,385 - RPSI07_RS08395 0.88 -0.4
118,491 - +0.6
118,535 - -1.4
118,711 - RPSI07_RS08400 0.18 -0.9
119,094 + RPSI07_RS08400 0.82 -0.3
119,094 + RPSI07_RS08400 0.82 +0.2
119,095 - RPSI07_RS08400 0.82 -0.9
119,151 + -2.5
119,151 + -1.0
119,256 - -1.4
119,304 + +1.0
119,304 + -0.8
119,400 + -0.6
119,434 + -1.3
119,434 + -0.0
119,434 + +0.4
119,435 - -0.3
119,693 + RPSI07_RS08405 0.16 -0.4
119,693 + RPSI07_RS08405 0.16 -1.0
119,693 + RPSI07_RS08405 0.16 -0.1
119,693 + RPSI07_RS08405 0.16 -2.8
119,693 + RPSI07_RS08405 0.16 +0.4
119,694 - RPSI07_RS08405 0.16 -2.1
119,694 - RPSI07_RS08405 0.16 +0.3
119,851 + RPSI07_RS08405 0.30 -0.2
119,852 - RPSI07_RS08405 0.30 -0.2
119,852 - RPSI07_RS08405 0.30 -0.9
120,012 - RPSI07_RS08405 0.44 -0.4
120,012 - RPSI07_RS08405 0.44 +0.3

Or see this region's nucleotide sequence