Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07285 and RPSI07_RS07290 are separated by 6 nucleotides RPSI07_RS07290 and RPSI07_RS07295 are separated by 135 nucleotides
RPSI07_RS07285: RPSI07_RS07285 - two-component sensor histidine kinase, at 1,933,748 to 1,935,064
_RS07285
RPSI07_RS07290: RPSI07_RS07290 - DNA-binding response regulator, at 1,935,071 to 1,935,811
_RS07290
RPSI07_RS07295: RPSI07_RS07295 - hypothetical protein, at 1,935,947 to 1,938,151
_RS07295
Position (kb)
1935
1936 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1934.215 kb on + strand, within RPSI07_RS07285 at 1934.215 kb on + strand, within RPSI07_RS07285 at 1934.215 kb on + strand, within RPSI07_RS07285 at 1934.215 kb on + strand, within RPSI07_RS07285 at 1934.499 kb on + strand, within RPSI07_RS07285 at 1934.499 kb on + strand, within RPSI07_RS07285 at 1934.499 kb on + strand, within RPSI07_RS07285 at 1934.682 kb on + strand, within RPSI07_RS07285 at 1934.682 kb on + strand, within RPSI07_RS07285 at 1934.682 kb on + strand, within RPSI07_RS07285 at 1934.683 kb on - strand, within RPSI07_RS07285 at 1934.683 kb on - strand, within RPSI07_RS07285 at 1934.683 kb on - strand, within RPSI07_RS07285 at 1934.727 kb on + strand, within RPSI07_RS07285 at 1934.728 kb on - strand, within RPSI07_RS07285 at 1934.728 kb on - strand, within RPSI07_RS07285 at 1934.814 kb on + strand, within RPSI07_RS07285 at 1934.814 kb on + strand, within RPSI07_RS07285 at 1934.814 kb on + strand, within RPSI07_RS07285 at 1934.815 kb on - strand, within RPSI07_RS07285 at 1934.815 kb on - strand, within RPSI07_RS07285 at 1934.815 kb on - strand, within RPSI07_RS07285 at 1934.815 kb on - strand, within RPSI07_RS07285 at 1934.815 kb on - strand, within RPSI07_RS07285 at 1934.815 kb on - strand, within RPSI07_RS07285 at 1934.982 kb on + strand at 1934.982 kb on + strand at 1934.982 kb on + strand at 1934.982 kb on + strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1934.983 kb on - strand at 1935.073 kb on - strand at 1935.135 kb on + strand at 1935.135 kb on + strand at 1935.135 kb on + strand at 1935.198 kb on + strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.199 kb on - strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.211 kb on + strand, within RPSI07_RS07290 at 1935.212 kb on - strand at 1935.212 kb on - strand, within RPSI07_RS07290 at 1935.212 kb on - strand, within RPSI07_RS07290 at 1935.212 kb on - strand, within RPSI07_RS07290 at 1935.240 kb on + strand, within RPSI07_RS07290 at 1935.241 kb on - strand, within RPSI07_RS07290 at 1935.483 kb on + strand, within RPSI07_RS07290 at 1935.483 kb on + strand, within RPSI07_RS07290 at 1935.484 kb on - strand, within RPSI07_RS07290 at 1935.803 kb on + strand at 1935.803 kb on + strand at 1935.803 kb on + strand at 1935.803 kb on + strand at 1935.803 kb on + strand at 1935.804 kb on - strand at 1935.848 kb on + strand at 1935.849 kb on - strand at 1935.849 kb on - strand at 1935.984 kb on + strand at 1935.985 kb on - strand at 1936.003 kb on + strand at 1936.003 kb on + strand at 1936.116 kb on + strand at 1936.116 kb on + strand at 1936.117 kb on - strand at 1936.117 kb on - strand at 1936.143 kb on + strand at 1936.144 kb on - strand at 1936.144 kb on - strand at 1936.144 kb on - strand at 1936.153 kb on + strand at 1936.154 kb on - strand at 1936.470 kb on + strand, within RPSI07_RS07295 at 1936.470 kb on + strand, within RPSI07_RS07295 at 1936.470 kb on + strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.471 kb on - strand, within RPSI07_RS07295 at 1936.737 kb on + strand, within RPSI07_RS07295 at 1936.737 kb on + strand, within RPSI07_RS07295 at 1936.738 kb on - strand, within RPSI07_RS07295 at 1936.738 kb on - strand, within RPSI07_RS07295 at 1936.767 kb on + strand, within RPSI07_RS07295 at 1936.767 kb on + strand, within RPSI07_RS07295 at 1936.767 kb on + strand, within RPSI07_RS07295 at 1936.768 kb on - strand, within RPSI07_RS07295 at 1936.768 kb on - strand, within RPSI07_RS07295 at 1936.768 kb on - strand, within RPSI07_RS07295
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,934,215 + RPSI07_RS07285 0.35 -0.1 1,934,215 + RPSI07_RS07285 0.35 +0.4 1,934,215 + RPSI07_RS07285 0.35 -0.1 1,934,215 + RPSI07_RS07285 0.35 +0.2 1,934,499 + RPSI07_RS07285 0.57 -2.2 1,934,499 + RPSI07_RS07285 0.57 +0.1 1,934,499 + RPSI07_RS07285 0.57 -1.2 1,934,682 + RPSI07_RS07285 0.71 +3.5 1,934,682 + RPSI07_RS07285 0.71 +0.7 1,934,682 + RPSI07_RS07285 0.71 +1.0 1,934,683 - RPSI07_RS07285 0.71 +0.1 1,934,683 - RPSI07_RS07285 0.71 +5.2 1,934,683 - RPSI07_RS07285 0.71 -0.5 1,934,727 + RPSI07_RS07285 0.74 -0.7 1,934,728 - RPSI07_RS07285 0.74 -0.3 1,934,728 - RPSI07_RS07285 0.74 -0.2 1,934,814 + RPSI07_RS07285 0.81 +1.3 1,934,814 + RPSI07_RS07285 0.81 +0.4 1,934,814 + RPSI07_RS07285 0.81 +1.7 1,934,815 - RPSI07_RS07285 0.81 -0.6 1,934,815 - RPSI07_RS07285 0.81 +0.1 1,934,815 - RPSI07_RS07285 0.81 +0.1 1,934,815 - RPSI07_RS07285 0.81 -0.9 1,934,815 - RPSI07_RS07285 0.81 -0.4 1,934,815 - RPSI07_RS07285 0.81 +0.9 1,934,982 + -0.5 1,934,982 + -0.4 1,934,982 + -1.1 1,934,982 + -0.6 1,934,983 - -1.3 1,934,983 - +2.3 1,934,983 - +0.1 1,934,983 - -0.0 1,934,983 - +0.8 1,934,983 - -0.4 1,935,073 - -0.4 1,935,135 + +0.6 1,935,135 + +0.1 1,935,135 + +0.2 1,935,198 + RPSI07_RS07290 0.17 +3.6 1,935,199 - RPSI07_RS07290 0.17 +1.0 1,935,199 - RPSI07_RS07290 0.17 -1.1 1,935,199 - RPSI07_RS07290 0.17 +1.0 1,935,199 - RPSI07_RS07290 0.17 -1.0 1,935,199 - RPSI07_RS07290 0.17 +1.3 1,935,199 - RPSI07_RS07290 0.17 -1.8 1,935,211 + RPSI07_RS07290 0.19 -2.5 1,935,211 + RPSI07_RS07290 0.19 +0.4 1,935,211 + RPSI07_RS07290 0.19 -3.0 1,935,211 + RPSI07_RS07290 0.19 -0.9 1,935,211 + RPSI07_RS07290 0.19 +1.2 1,935,211 + RPSI07_RS07290 0.19 -0.2 1,935,212 - -1.9 1,935,212 - RPSI07_RS07290 0.19 -0.1 1,935,212 - RPSI07_RS07290 0.19 +1.5 1,935,212 - RPSI07_RS07290 0.19 +0.0 1,935,240 + RPSI07_RS07290 0.23 +1.3 1,935,241 - RPSI07_RS07290 0.23 +1.7 1,935,483 + RPSI07_RS07290 0.56 -2.4 1,935,483 + RPSI07_RS07290 0.56 +0.2 1,935,484 - RPSI07_RS07290 0.56 -0.9 1,935,803 + +0.4 1,935,803 + -1.1 1,935,803 + -0.6 1,935,803 + -1.3 1,935,803 + +0.1 1,935,804 - -2.1 1,935,848 + +0.3 1,935,849 - -0.5 1,935,849 - -0.6 1,935,984 + -0.4 1,935,985 - +2.3 1,936,003 + -2.3 1,936,003 + +1.2 1,936,116 + +0.9 1,936,116 + -1.5 1,936,117 - -0.9 1,936,117 - +1.7 1,936,143 + -1.3 1,936,144 - +4.2 1,936,144 - -0.5 1,936,144 - +0.5 1,936,153 + -0.5 1,936,154 - +0.3 1,936,470 + RPSI07_RS07295 0.24 +1.7 1,936,470 + RPSI07_RS07295 0.24 -0.3 1,936,470 + RPSI07_RS07295 0.24 -0.3 1,936,471 - RPSI07_RS07295 0.24 -0.6 1,936,471 - RPSI07_RS07295 0.24 +3.3 1,936,471 - RPSI07_RS07295 0.24 +0.4 1,936,471 - RPSI07_RS07295 0.24 -0.5 1,936,471 - RPSI07_RS07295 0.24 -0.2 1,936,737 + RPSI07_RS07295 0.36 +0.3 1,936,737 + RPSI07_RS07295 0.36 +0.7 1,936,738 - RPSI07_RS07295 0.36 +1.7 1,936,738 - RPSI07_RS07295 0.36 +0.7 1,936,767 + RPSI07_RS07295 0.37 -0.2 1,936,767 + RPSI07_RS07295 0.37 +0.1 1,936,767 + RPSI07_RS07295 0.37 -0.8 1,936,768 - RPSI07_RS07295 0.37 -1.0 1,936,768 - RPSI07_RS07295 0.37 +0.5 1,936,768 - RPSI07_RS07295 0.37 -0.6
Or see this region's nucleotide sequence