Strain Fitness in Escherichia coli BL21 around ECD_03000

Experiment: Control_TnJ

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02998 and ECD_02999 are separated by 10 nucleotidesECD_02999 and ECD_03000 overlap by 11 nucleotidesECD_03000 and ECD_03001 are separated by 17 nucleotidesECD_03001 and ECD_03002 overlap by 4 nucleotides ECD_02998: ECD_02998 - N-acetylgalactosamine-specific enzyme IIB component of PTS, at 3,146,410 to 3,146,883 _02998 ECD_02999: ECD_02999 - N-acetylgalactosamine-specific enzyme IIC component of PTS, at 3,146,894 to 3,147,673 _02999 ECD_03000: ECD_03000 - PTS system, N-acetylgalactosamine-specific IID component, at 3,147,663 to 3,148,541 _03000 ECD_03001: ECD_03001 - putative phosphotransferase system enzyme subunit (N-acetyl-galactosamine/galactosamine PTS system enzyme IIA component), at 3,148,559 to 3,148,993 _03001 ECD_03002: ECD_03002 - N-acetylgalactosamine-6-phosphate deacetylase, at 3,148,990 to 3,150,123 _03002 Position (kb) 3147 3148 3149Strain fitness (log2 ratio) -2 -1 0 1 2at 3146.728 kb on - strand, within ECD_02998at 3146.734 kb on + strand, within ECD_02998at 3146.876 kb on + strandat 3146.977 kb on + strand, within ECD_02999at 3146.977 kb on + strand, within ECD_02999at 3146.977 kb on + strand, within ECD_02999at 3146.977 kb on + strand, within ECD_02999at 3146.978 kb on - strand, within ECD_02999at 3146.978 kb on - strand, within ECD_02999at 3146.978 kb on - strand, within ECD_02999at 3146.978 kb on - strand, within ECD_02999at 3146.978 kb on - strand, within ECD_02999at 3147.127 kb on + strand, within ECD_02999at 3147.211 kb on - strand, within ECD_02999at 3147.223 kb on + strand, within ECD_02999at 3147.223 kb on + strand, within ECD_02999at 3147.224 kb on - strand, within ECD_02999at 3147.224 kb on - strand, within ECD_02999at 3147.315 kb on - strand, within ECD_02999at 3147.368 kb on - strand, within ECD_02999at 3147.371 kb on + strand, within ECD_02999at 3147.372 kb on - strand, within ECD_02999at 3147.372 kb on - strand, within ECD_02999at 3147.372 kb on - strand, within ECD_02999at 3147.372 kb on - strand, within ECD_02999at 3147.372 kb on - strand, within ECD_02999at 3147.516 kb on - strand, within ECD_02999at 3147.613 kb on + strandat 3147.783 kb on - strand, within ECD_03000at 3147.814 kb on + strand, within ECD_03000at 3147.827 kb on + strand, within ECD_03000at 3147.839 kb on + strand, within ECD_03000at 3147.839 kb on + strand, within ECD_03000at 3147.839 kb on + strand, within ECD_03000at 3147.840 kb on - strand, within ECD_03000at 3147.896 kb on + strand, within ECD_03000at 3147.900 kb on - strand, within ECD_03000at 3147.900 kb on - strand, within ECD_03000at 3148.004 kb on + strand, within ECD_03000at 3148.004 kb on + strand, within ECD_03000at 3148.005 kb on - strand, within ECD_03000at 3148.005 kb on - strand, within ECD_03000at 3148.093 kb on + strand, within ECD_03000at 3148.184 kb on - strand, within ECD_03000at 3148.215 kb on + strand, within ECD_03000at 3148.215 kb on + strand, within ECD_03000at 3148.216 kb on - strand, within ECD_03000at 3148.221 kb on + strand, within ECD_03000at 3148.262 kb on + strand, within ECD_03000at 3148.306 kb on + strand, within ECD_03000at 3148.329 kb on + strand, within ECD_03000at 3148.329 kb on + strand, within ECD_03000at 3148.329 kb on + strand, within ECD_03000at 3148.329 kb on + strand, within ECD_03000at 3148.329 kb on + strand, within ECD_03000at 3148.330 kb on - strand, within ECD_03000at 3148.330 kb on - strand, within ECD_03000at 3148.330 kb on - strand, within ECD_03000at 3148.330 kb on - strand, within ECD_03000at 3148.339 kb on + strand, within ECD_03000at 3148.379 kb on + strand, within ECD_03000at 3148.379 kb on + strand, within ECD_03000at 3148.385 kb on - strand, within ECD_03000at 3148.496 kb on + strandat 3148.496 kb on + strandat 3148.544 kb on - strandat 3148.556 kb on + strandat 3148.690 kb on + strand, within ECD_03001at 3148.691 kb on - strand, within ECD_03001at 3148.989 kb on + strandat 3149.058 kb on + strandat 3149.059 kb on - strandat 3149.140 kb on + strand, within ECD_03002at 3149.141 kb on - strand, within ECD_03002at 3149.156 kb on + strand, within ECD_03002at 3149.157 kb on - strand, within ECD_03002at 3149.180 kb on + strand, within ECD_03002at 3149.180 kb on + strand, within ECD_03002at 3149.180 kb on + strand, within ECD_03002at 3149.180 kb on + strand, within ECD_03002at 3149.180 kb on + strand, within ECD_03002at 3149.180 kb on + strand, within ECD_03002at 3149.181 kb on - strand, within ECD_03002at 3149.181 kb on - strand, within ECD_03002at 3149.201 kb on + strand, within ECD_03002at 3149.201 kb on + strand, within ECD_03002at 3149.201 kb on + strand, within ECD_03002at 3149.201 kb on + strand, within ECD_03002at 3149.202 kb on - strand, within ECD_03002at 3149.202 kb on - strand, within ECD_03002at 3149.304 kb on + strand, within ECD_03002at 3149.305 kb on - strand, within ECD_03002at 3149.305 kb on - strand, within ECD_03002at 3149.356 kb on + strand, within ECD_03002at 3149.357 kb on - strand, within ECD_03002at 3149.372 kb on - strand, within ECD_03002at 3149.388 kb on + strand, within ECD_03002at 3149.529 kb on + strand, within ECD_03002at 3149.530 kb on - strand, within ECD_03002

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_TnJ
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3,146,728 - ECD_02998 0.67 -0.6
3,146,734 + ECD_02998 0.68 -2.3
3,146,876 + -0.1
3,146,977 + ECD_02999 0.11 +0.5
3,146,977 + ECD_02999 0.11 -1.3
3,146,977 + ECD_02999 0.11 +0.1
3,146,977 + ECD_02999 0.11 +1.4
3,146,978 - ECD_02999 0.11 -0.8
3,146,978 - ECD_02999 0.11 +0.1
3,146,978 - ECD_02999 0.11 +0.4
3,146,978 - ECD_02999 0.11 +0.1
3,146,978 - ECD_02999 0.11 +1.1
3,147,127 + ECD_02999 0.30 -0.9
3,147,211 - ECD_02999 0.41 +0.3
3,147,223 + ECD_02999 0.42 +0.1
3,147,223 + ECD_02999 0.42 +0.0
3,147,224 - ECD_02999 0.42 +0.2
3,147,224 - ECD_02999 0.42 +0.4
3,147,315 - ECD_02999 0.54 -0.5
3,147,368 - ECD_02999 0.61 +0.1
3,147,371 + ECD_02999 0.61 -0.4
3,147,372 - ECD_02999 0.61 +0.4
3,147,372 - ECD_02999 0.61 -0.0
3,147,372 - ECD_02999 0.61 +0.4
3,147,372 - ECD_02999 0.61 +0.9
3,147,372 - ECD_02999 0.61 -0.2
3,147,516 - ECD_02999 0.80 -2.3
3,147,613 + +0.0
3,147,783 - ECD_03000 0.14 +0.6
3,147,814 + ECD_03000 0.17 +0.1
3,147,827 + ECD_03000 0.19 +0.4
3,147,839 + ECD_03000 0.20 +0.7
3,147,839 + ECD_03000 0.20 +1.0
3,147,839 + ECD_03000 0.20 -0.1
3,147,840 - ECD_03000 0.20 -0.7
3,147,896 + ECD_03000 0.27 +0.1
3,147,900 - ECD_03000 0.27 +0.5
3,147,900 - ECD_03000 0.27 -0.6
3,148,004 + ECD_03000 0.39 -0.2
3,148,004 + ECD_03000 0.39 +0.2
3,148,005 - ECD_03000 0.39 +1.8
3,148,005 - ECD_03000 0.39 +0.1
3,148,093 + ECD_03000 0.49 +0.3
3,148,184 - ECD_03000 0.59 +0.7
3,148,215 + ECD_03000 0.63 -0.7
3,148,215 + ECD_03000 0.63 +0.6
3,148,216 - ECD_03000 0.63 +1.6
3,148,221 + ECD_03000 0.63 +1.6
3,148,262 + ECD_03000 0.68 +0.2
3,148,306 + ECD_03000 0.73 +0.2
3,148,329 + ECD_03000 0.76 -0.5
3,148,329 + ECD_03000 0.76 +0.5
3,148,329 + ECD_03000 0.76 +0.1
3,148,329 + ECD_03000 0.76 -0.8
3,148,329 + ECD_03000 0.76 +0.7
3,148,330 - ECD_03000 0.76 +0.1
3,148,330 - ECD_03000 0.76 +1.3
3,148,330 - ECD_03000 0.76 -0.6
3,148,330 - ECD_03000 0.76 -1.3
3,148,339 + ECD_03000 0.77 +0.0
3,148,379 + ECD_03000 0.81 +1.2
3,148,379 + ECD_03000 0.81 +0.1
3,148,385 - ECD_03000 0.82 +0.3
3,148,496 + -0.1
3,148,496 + +0.9
3,148,544 - -0.5
3,148,556 + +0.2
3,148,690 + ECD_03001 0.30 -0.3
3,148,691 - ECD_03001 0.30 -0.5
3,148,989 + +0.0
3,149,058 + +0.1
3,149,059 - -1.4
3,149,140 + ECD_03002 0.13 +0.4
3,149,141 - ECD_03002 0.13 -0.4
3,149,156 + ECD_03002 0.15 +0.9
3,149,157 - ECD_03002 0.15 -0.2
3,149,180 + ECD_03002 0.17 +0.2
3,149,180 + ECD_03002 0.17 -0.3
3,149,180 + ECD_03002 0.17 +0.1
3,149,180 + ECD_03002 0.17 -1.5
3,149,180 + ECD_03002 0.17 -0.3
3,149,180 + ECD_03002 0.17 -1.7
3,149,181 - ECD_03002 0.17 +0.1
3,149,181 - ECD_03002 0.17 -0.4
3,149,201 + ECD_03002 0.19 -2.1
3,149,201 + ECD_03002 0.19 -0.3
3,149,201 + ECD_03002 0.19 -0.4
3,149,201 + ECD_03002 0.19 +1.0
3,149,202 - ECD_03002 0.19 -0.1
3,149,202 - ECD_03002 0.19 -0.5
3,149,304 + ECD_03002 0.28 +0.7
3,149,305 - ECD_03002 0.28 +0.5
3,149,305 - ECD_03002 0.28 +0.3
3,149,356 + ECD_03002 0.32 +0.8
3,149,357 - ECD_03002 0.32 +1.6
3,149,372 - ECD_03002 0.34 -0.5
3,149,388 + ECD_03002 0.35 +0.2
3,149,529 + ECD_03002 0.48 +1.2
3,149,530 - ECD_03002 0.48 +0.1

Or see this region's nucleotide sequence