Strain Fitness in Escherichia coli BL21 around ECD_00397

Experiment: Control_TnJ

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00395 and ECD_00396 are separated by 51 nucleotidesECD_00396 and ECD_00397 are separated by 64 nucleotidesECD_00397 and ECD_00398 are separated by 99 nucleotides ECD_00395: ECD_00395 - long-chain acyl-CoA thioesterase III, at 433,448 to 433,846 _00395 ECD_00396: ECD_00396 - 7-cyano-7-deazaguanine (preQ0) synthase; queuosine biosynthesis, at 433,898 to 434,593 _00396 ECD_00397: ECD_00397 - putative ABC transporter periplasmic binding protein, at 434,658 to 436,358 _00397 ECD_00398: ECD_00398 - thiamine pyrimidine pyrophosphate hydrolase; HMP-PP phosphatase, at 436,458 to 437,276 _00398 Position (kb) 434 435 436 437Strain fitness (log2 ratio) -2 -1 0 1 2at 433.660 kb on + strand, within ECD_00395at 433.661 kb on - strand, within ECD_00395at 433.661 kb on - strand, within ECD_00395at 433.664 kb on - strand, within ECD_00395at 433.664 kb on - strand, within ECD_00395at 433.704 kb on + strand, within ECD_00395at 433.717 kb on + strand, within ECD_00395at 433.780 kb on + strand, within ECD_00395at 433.900 kb on - strandat 433.956 kb on + strandat 433.956 kb on + strandat 434.029 kb on - strand, within ECD_00396at 434.265 kb on + strand, within ECD_00396at 434.265 kb on + strand, within ECD_00396at 434.265 kb on + strand, within ECD_00396at 434.265 kb on + strand, within ECD_00396at 434.265 kb on + strand, within ECD_00396at 434.266 kb on - strand, within ECD_00396at 434.266 kb on - strand, within ECD_00396at 434.266 kb on - strand, within ECD_00396at 434.266 kb on - strand, within ECD_00396at 434.267 kb on + strand, within ECD_00396at 434.294 kb on - strand, within ECD_00396at 434.294 kb on - strand, within ECD_00396at 434.406 kb on - strand, within ECD_00396at 434.406 kb on - strand, within ECD_00396at 434.419 kb on + strand, within ECD_00396at 434.520 kb on + strand, within ECD_00396at 434.521 kb on - strand, within ECD_00396at 434.521 kb on - strand, within ECD_00396at 434.529 kb on - strandat 434.529 kb on - strandat 434.655 kb on + strandat 434.656 kb on - strandat 434.748 kb on - strandat 434.796 kb on + strandat 434.826 kb on - strandat 434.850 kb on + strand, within ECD_00397at 434.887 kb on + strand, within ECD_00397at 434.887 kb on + strand, within ECD_00397at 434.888 kb on - strand, within ECD_00397at 434.888 kb on - strand, within ECD_00397at 434.888 kb on - strand, within ECD_00397at 434.949 kb on - strand, within ECD_00397at 434.949 kb on - strand, within ECD_00397at 434.950 kb on + strand, within ECD_00397at 434.970 kb on + strand, within ECD_00397at 435.007 kb on - strand, within ECD_00397at 435.117 kb on + strand, within ECD_00397at 435.147 kb on + strand, within ECD_00397at 435.182 kb on + strand, within ECD_00397at 435.182 kb on + strand, within ECD_00397at 435.238 kb on - strand, within ECD_00397at 435.324 kb on + strand, within ECD_00397at 435.325 kb on - strand, within ECD_00397at 435.476 kb on + strand, within ECD_00397at 435.477 kb on - strand, within ECD_00397at 435.502 kb on + strand, within ECD_00397at 435.503 kb on - strand, within ECD_00397at 435.604 kb on + strand, within ECD_00397at 435.605 kb on - strand, within ECD_00397at 435.684 kb on - strand, within ECD_00397at 435.703 kb on - strand, within ECD_00397at 435.738 kb on + strand, within ECD_00397at 435.830 kb on + strand, within ECD_00397at 435.875 kb on + strand, within ECD_00397at 435.876 kb on - strand, within ECD_00397at 435.879 kb on - strand, within ECD_00397at 435.906 kb on + strand, within ECD_00397at 435.906 kb on + strand, within ECD_00397at 435.980 kb on - strand, within ECD_00397at 436.025 kb on + strand, within ECD_00397at 436.158 kb on + strand, within ECD_00397at 436.159 kb on - strand, within ECD_00397at 436.378 kb on - strandat 436.396 kb on - strandat 436.403 kb on + strandat 436.403 kb on + strandat 436.403 kb on + strandat 436.403 kb on + strandat 436.403 kb on + strandat 436.403 kb on + strandat 436.404 kb on - strandat 436.404 kb on - strandat 436.405 kb on + strandat 436.405 kb on + strandat 436.417 kb on + strandat 436.517 kb on - strandat 436.639 kb on - strand, within ECD_00398at 436.691 kb on - strand, within ECD_00398at 436.705 kb on + strand, within ECD_00398at 436.767 kb on - strand, within ECD_00398at 436.923 kb on - strand, within ECD_00398at 436.944 kb on + strand, within ECD_00398at 436.944 kb on + strand, within ECD_00398at 437.021 kb on + strand, within ECD_00398at 437.063 kb on - strand, within ECD_00398at 437.138 kb on + strand, within ECD_00398at 437.139 kb on - strand, within ECD_00398at 437.227 kb on - strandat 437.257 kb on + strandat 437.257 kb on + strandat 437.258 kb on - strandat 437.258 kb on - strandat 437.260 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_TnJ
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433,660 + ECD_00395 0.53 +0.1
433,661 - ECD_00395 0.53 -0.7
433,661 - ECD_00395 0.53 +0.1
433,664 - ECD_00395 0.54 -1.0
433,664 - ECD_00395 0.54 -0.6
433,704 + ECD_00395 0.64 +0.7
433,717 + ECD_00395 0.67 +0.3
433,780 + ECD_00395 0.83 -0.3
433,900 - +0.5
433,956 + -0.8
433,956 + -1.1
434,029 - ECD_00396 0.19 -0.6
434,265 + ECD_00396 0.53 -0.5
434,265 + ECD_00396 0.53 -0.6
434,265 + ECD_00396 0.53 +0.8
434,265 + ECD_00396 0.53 +1.2
434,265 + ECD_00396 0.53 +0.0
434,266 - ECD_00396 0.53 -0.2
434,266 - ECD_00396 0.53 +1.0
434,266 - ECD_00396 0.53 -0.7
434,266 - ECD_00396 0.53 -0.2
434,267 + ECD_00396 0.53 +0.9
434,294 - ECD_00396 0.57 +0.5
434,294 - ECD_00396 0.57 +0.2
434,406 - ECD_00396 0.73 -0.4
434,406 - ECD_00396 0.73 -1.1
434,419 + ECD_00396 0.75 +0.0
434,520 + ECD_00396 0.89 -0.5
434,521 - ECD_00396 0.90 -0.3
434,521 - ECD_00396 0.90 +0.1
434,529 - +0.4
434,529 - +1.3
434,655 + +0.9
434,656 - +1.7
434,748 - -1.2
434,796 + +1.5
434,826 - -1.3
434,850 + ECD_00397 0.11 +0.3
434,887 + ECD_00397 0.13 +0.7
434,887 + ECD_00397 0.13 +0.1
434,888 - ECD_00397 0.14 +1.5
434,888 - ECD_00397 0.14 -0.3
434,888 - ECD_00397 0.14 +0.2
434,949 - ECD_00397 0.17 -2.0
434,949 - ECD_00397 0.17 +0.8
434,950 + ECD_00397 0.17 +0.2
434,970 + ECD_00397 0.18 +0.5
435,007 - ECD_00397 0.21 +0.0
435,117 + ECD_00397 0.27 +0.0
435,147 + ECD_00397 0.29 +0.3
435,182 + ECD_00397 0.31 +1.8
435,182 + ECD_00397 0.31 -0.9
435,238 - ECD_00397 0.34 -0.1
435,324 + ECD_00397 0.39 -0.0
435,325 - ECD_00397 0.39 -0.0
435,476 + ECD_00397 0.48 -1.0
435,477 - ECD_00397 0.48 -2.0
435,502 + ECD_00397 0.50 -0.5
435,503 - ECD_00397 0.50 +0.2
435,604 + ECD_00397 0.56 +0.2
435,605 - ECD_00397 0.56 +0.5
435,684 - ECD_00397 0.60 +0.2
435,703 - ECD_00397 0.61 -0.2
435,738 + ECD_00397 0.63 +0.9
435,830 + ECD_00397 0.69 +0.3
435,875 + ECD_00397 0.72 -1.1
435,876 - ECD_00397 0.72 +2.0
435,879 - ECD_00397 0.72 -0.4
435,906 + ECD_00397 0.73 +1.6
435,906 + ECD_00397 0.73 -0.2
435,980 - ECD_00397 0.78 +0.9
436,025 + ECD_00397 0.80 -1.1
436,158 + ECD_00397 0.88 +0.2
436,159 - ECD_00397 0.88 +0.7
436,378 - +0.3
436,396 - -0.9
436,403 + +0.5
436,403 + -0.9
436,403 + +0.7
436,403 + -1.1
436,403 + -0.4
436,403 + +0.1
436,404 - +0.0
436,404 - +0.3
436,405 + +0.4
436,405 + +0.7
436,417 + -0.5
436,517 - +0.8
436,639 - ECD_00398 0.22 +0.3
436,691 - ECD_00398 0.28 -0.3
436,705 + ECD_00398 0.30 +1.2
436,767 - ECD_00398 0.38 +1.2
436,923 - ECD_00398 0.57 -0.9
436,944 + ECD_00398 0.59 -0.4
436,944 + ECD_00398 0.59 -0.6
437,021 + ECD_00398 0.69 +1.0
437,063 - ECD_00398 0.74 +0.2
437,138 + ECD_00398 0.83 +0.2
437,139 - ECD_00398 0.83 -1.0
437,227 - -0.5
437,257 + -0.5
437,257 + +0.3
437,258 - +0.3
437,258 - +0.6
437,260 - -0.0

Or see this region's nucleotide sequence