Strain Fitness in Escherichia coli BL21 around ECD_00280

Experiment: Control_TnJ

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00279 and ECD_00280 are separated by 376 nucleotidesECD_00280 and ECD_00281 are separated by 243 nucleotides ECD_00279: ECD_00279 - putative metallo-dependent hydrolase domain deaminase, at 310,753 to 312,135 _00279 ECD_00280: ECD_00280 - broad specificity NADPH-dependent aldehyde reductase, Zn-containing, at 312,512 to 313,561 _00280 ECD_00281: ECD_00281 - uncharacterized protein, at 313,805 to 314,620 _00281 Position (kb) 312 313 314Strain fitness (log2 ratio) -2 -1 0 1 2at 311.617 kb on + strand, within ECD_00279at 311.617 kb on + strand, within ECD_00279at 311.618 kb on - strand, within ECD_00279at 311.618 kb on - strand, within ECD_00279at 311.618 kb on - strand, within ECD_00279at 311.618 kb on - strand, within ECD_00279at 311.618 kb on - strand, within ECD_00279at 311.711 kb on + strand, within ECD_00279at 311.751 kb on + strand, within ECD_00279at 311.751 kb on + strand, within ECD_00279at 311.784 kb on + strand, within ECD_00279at 311.863 kb on + strand, within ECD_00279at 311.864 kb on - strand, within ECD_00279at 311.899 kb on + strand, within ECD_00279at 311.911 kb on + strand, within ECD_00279at 311.912 kb on - strand, within ECD_00279at 311.912 kb on - strand, within ECD_00279at 311.969 kb on + strand, within ECD_00279at 311.970 kb on - strand, within ECD_00279at 311.970 kb on - strand, within ECD_00279at 312.072 kb on + strandat 312.073 kb on - strandat 312.165 kb on + strandat 312.165 kb on + strandat 312.168 kb on + strandat 312.168 kb on + strandat 312.177 kb on + strandat 312.177 kb on + strandat 312.206 kb on - strandat 312.233 kb on - strandat 312.266 kb on + strandat 312.274 kb on + strandat 312.274 kb on + strandat 312.321 kb on + strandat 312.334 kb on + strandat 312.343 kb on + strandat 312.423 kb on + strandat 312.427 kb on + strandat 312.434 kb on + strandat 312.434 kb on + strandat 312.434 kb on + strandat 312.435 kb on - strandat 312.468 kb on - strandat 312.484 kb on + strandat 312.484 kb on + strandat 312.490 kb on + strandat 312.490 kb on + strandat 312.490 kb on + strandat 312.490 kb on + strandat 312.491 kb on - strandat 312.491 kb on - strandat 312.491 kb on - strandat 312.536 kb on + strandat 312.851 kb on + strand, within ECD_00280at 312.852 kb on - strand, within ECD_00280at 312.852 kb on - strand, within ECD_00280at 312.853 kb on + strand, within ECD_00280at 312.883 kb on + strand, within ECD_00280at 313.241 kb on - strand, within ECD_00280at 313.245 kb on + strand, within ECD_00280at 313.258 kb on + strand, within ECD_00280at 313.273 kb on + strand, within ECD_00280at 313.388 kb on - strand, within ECD_00280at 313.484 kb on - strandat 313.493 kb on + strandat 314.096 kb on + strand, within ECD_00281at 314.177 kb on + strand, within ECD_00281at 314.348 kb on - strand, within ECD_00281at 314.383 kb on + strand, within ECD_00281at 314.513 kb on + strand, within ECD_00281

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_TnJ
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311,617 + ECD_00279 0.62 +0.8
311,617 + ECD_00279 0.62 +1.3
311,618 - ECD_00279 0.63 +0.2
311,618 - ECD_00279 0.63 +0.1
311,618 - ECD_00279 0.63 -0.0
311,618 - ECD_00279 0.63 -0.5
311,618 - ECD_00279 0.63 +1.1
311,711 + ECD_00279 0.69 -0.3
311,751 + ECD_00279 0.72 +0.6
311,751 + ECD_00279 0.72 +0.5
311,784 + ECD_00279 0.75 +0.3
311,863 + ECD_00279 0.80 +0.8
311,864 - ECD_00279 0.80 +1.3
311,899 + ECD_00279 0.83 +0.2
311,911 + ECD_00279 0.84 +0.3
311,912 - ECD_00279 0.84 +1.0
311,912 - ECD_00279 0.84 -0.8
311,969 + ECD_00279 0.88 -0.7
311,970 - ECD_00279 0.88 +1.3
311,970 - ECD_00279 0.88 -2.3
312,072 + -0.5
312,073 - +0.4
312,165 + -0.7
312,165 + +0.7
312,168 + +0.5
312,168 + -0.2
312,177 + -0.6
312,177 + -0.4
312,206 - +0.5
312,233 - +0.8
312,266 + +0.2
312,274 + +0.2
312,274 + +0.0
312,321 + -0.2
312,334 + -0.7
312,343 + +1.3
312,423 + -0.3
312,427 + -0.1
312,434 + -1.1
312,434 + -0.7
312,434 + +1.1
312,435 - -0.6
312,468 - +1.7
312,484 + +1.7
312,484 + +1.0
312,490 + +1.1
312,490 + -0.8
312,490 + -0.3
312,490 + +0.4
312,491 - +0.5
312,491 - -0.2
312,491 - +0.3
312,536 + -0.5
312,851 + ECD_00280 0.32 +0.0
312,852 - ECD_00280 0.32 -1.0
312,852 - ECD_00280 0.32 -0.1
312,853 + ECD_00280 0.32 -1.0
312,883 + ECD_00280 0.35 +0.5
313,241 - ECD_00280 0.69 -0.0
313,245 + ECD_00280 0.70 -0.9
313,258 + ECD_00280 0.71 -1.8
313,273 + ECD_00280 0.72 -0.2
313,388 - ECD_00280 0.83 -0.6
313,484 - +0.6
313,493 + +2.0
314,096 + ECD_00281 0.36 -1.2
314,177 + ECD_00281 0.46 +1.2
314,348 - ECD_00281 0.67 -0.7
314,383 + ECD_00281 0.71 +0.1
314,513 + ECD_00281 0.87 -1.0

Or see this region's nucleotide sequence