Strain Fitness in Escherichia coli BL21 around ECD_00502

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00498 and ECD_00499 overlap by 8 nucleotidesECD_00499 and ECD_00500 overlap by 4 nucleotidesECD_00500 and ECD_00501 overlap by 4 nucleotidesECD_00501 and ECD_00502 are separated by 85 nucleotidesECD_00502 and ECD_00504 are separated by 717 nucleotides ECD_00498: ECD_00498 - DLP12 prophage; conserved protein, at 541,516 to 541,686 _00498 ECD_00499: ECD_00499 - DLP12 prophage; uncharacterized protein, at 541,679 to 541,969 _00499 ECD_00500: ECD_00500 - DLP12 prophage; endonuclease RUS, at 541,966 to 542,328 _00500 ECD_00501: ECD_00501 - uncharacterized protein, DLP12 prophage, at 542,325 to 542,465 _00501 ECD_00502: ECD_00502 - DLP12 prophage; putative antitermination protein, at 542,551 to 542,934 _00502 ECD_00504: ECD_00504 - IS1 protein InsB, at 543,652 to 544,155 _00504 Position (kb) 542 543Strain fitness (log2 ratio) -2 -1 0 1at 541.555 kb on + strand, within ECD_00498at 541.556 kb on - strand, within ECD_00498at 541.556 kb on - strand, within ECD_00498at 541.562 kb on + strand, within ECD_00498at 541.563 kb on - strand, within ECD_00498at 541.575 kb on + strand, within ECD_00498at 541.575 kb on + strand, within ECD_00498at 541.582 kb on - strand, within ECD_00498at 541.627 kb on - strand, within ECD_00498at 541.742 kb on - strand, within ECD_00499at 541.918 kb on + strand, within ECD_00499at 541.956 kb on + strandat 541.980 kb on + strandat 542.014 kb on + strand, within ECD_00500at 542.015 kb on - strand, within ECD_00500at 542.122 kb on - strand, within ECD_00500at 542.343 kb on - strand, within ECD_00501at 542.383 kb on + strand, within ECD_00501at 542.384 kb on - strand, within ECD_00501at 542.384 kb on - strand, within ECD_00501at 542.486 kb on + strandat 542.545 kb on - strandat 542.774 kb on - strand, within ECD_00502at 543.126 kb on - strandat 543.206 kb on - strandat 543.258 kb on + strandat 543.275 kb on + strandat 543.283 kb on + strandat 543.449 kb on + strandat 543.469 kb on - strandat 543.503 kb on - strandat 543.546 kb on - strandat 543.608 kb on + strandat 543.615 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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541,555 + ECD_00498 0.23 -0.4
541,556 - ECD_00498 0.23 -1.1
541,556 - ECD_00498 0.23 +0.5
541,562 + ECD_00498 0.27 -0.3
541,563 - ECD_00498 0.27 -0.1
541,575 + ECD_00498 0.35 +0.0
541,575 + ECD_00498 0.35 -2.1
541,582 - ECD_00498 0.39 -0.3
541,627 - ECD_00498 0.65 +0.9
541,742 - ECD_00499 0.22 +0.3
541,918 + ECD_00499 0.82 +0.6
541,956 + +1.5
541,980 + +0.1
542,014 + ECD_00500 0.13 -0.1
542,015 - ECD_00500 0.13 +1.0
542,122 - ECD_00500 0.43 -0.6
542,343 - ECD_00501 0.13 -0.1
542,383 + ECD_00501 0.41 +1.0
542,384 - ECD_00501 0.42 -1.1
542,384 - ECD_00501 0.42 +0.2
542,486 + +0.0
542,545 - +0.2
542,774 - ECD_00502 0.58 +0.7
543,126 - +0.9
543,206 - -0.4
543,258 + +0.7
543,275 + -0.0
543,283 + -0.7
543,449 + +1.4
543,469 - -0.7
543,503 - -0.7
543,546 - +0.4
543,608 + -0.8
543,615 - +1.2

Or see this region's nucleotide sequence