Strain Fitness in Escherichia coli BL21 around ECD_02075

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02073 and ECD_02074 are separated by 2 nucleotidesECD_02074 and ECD_02075 are separated by 193 nucleotidesECD_02075 and ECD_02076 overlap by 7 nucleotides ECD_02073: ECD_02073 - DUF218 superfamily vancomycin high temperature exclusion protein, at 2,127,782 to 2,128,501 _02073 ECD_02074: ECD_02074 - DUF2542 family protein, at 2,128,504 to 2,128,743 _02074 ECD_02075: ECD_02075 - dihydropyrimidine dehydrogenase, NADH-dependent, subunit N, at 2,128,937 to 2,130,175 _02075 ECD_02076: ECD_02076 - dihydropyrimidine dehydrogenase, NADH-dependent, subunit C, at 2,130,169 to 2,131,404 _02076 Position (kb) 2128 2129 2130 2131Strain fitness (log2 ratio) -1 0 1at 2127.975 kb on - strand, within ECD_02073at 2128.010 kb on + strand, within ECD_02073at 2128.035 kb on + strand, within ECD_02073at 2128.315 kb on - strand, within ECD_02073at 2129.706 kb on + strand, within ECD_02075at 2129.800 kb on - strand, within ECD_02075at 2129.800 kb on - strand, within ECD_02075at 2129.916 kb on + strand, within ECD_02075at 2129.916 kb on + strand, within ECD_02075at 2129.916 kb on + strand, within ECD_02075at 2129.985 kb on + strand, within ECD_02075at 2130.150 kb on - strandat 2130.643 kb on + strand, within ECD_02076at 2130.801 kb on + strand, within ECD_02076

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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2,127,975 - ECD_02073 0.27 +0.4
2,128,010 + ECD_02073 0.32 -0.7
2,128,035 + ECD_02073 0.35 -1.5
2,128,315 - ECD_02073 0.74 -0.8
2,129,706 + ECD_02075 0.62 +1.7
2,129,800 - ECD_02075 0.70 -0.1
2,129,800 - ECD_02075 0.70 +1.4
2,129,916 + ECD_02075 0.79 +0.1
2,129,916 + ECD_02075 0.79 +1.6
2,129,916 + ECD_02075 0.79 +0.1
2,129,985 + ECD_02075 0.85 -0.7
2,130,150 - -0.1
2,130,643 + ECD_02076 0.38 +1.0
2,130,801 + ECD_02076 0.51 +0.0

Or see this region's nucleotide sequence