Strain Fitness in Escherichia coli BL21 around ECD_01067

Experiment: JK16

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01065 and ECD_01066 are separated by 39 nucleotidesECD_01066 and ECD_01067 are separated by 4 nucleotidesECD_01067 and ECD_01068 are separated by 75 nucleotidesECD_01068 and ECD_01069 are separated by 154 nucleotides ECD_01065: ECD_01065 - putative lipid II flippase, at 1,130,409 to 1,131,944 _01065 ECD_01066: ECD_01066 - export chaperone for FlgK and FlgL, at 1,131,984 to 1,132,400 _01066 ECD_01067: ECD_01067 - anti-sigma factor for FliA (sigma 28), at 1,132,405 to 1,132,698 _01067 ECD_01068: ECD_01068 - assembly protein for flagellar basal-body periplasmic P ring, at 1,132,774 to 1,133,433 _01068 ECD_01069: ECD_01069 - flagellar component of cell-proximal portion of basal-body rod, at 1,133,588 to 1,134,004 _01069 Position (kb) 1132 1133Strain fitness (log2 ratio) -2 -1 0 1at 1131.942 kb on + strandat 1132.045 kb on - strand, within ECD_01066at 1132.125 kb on - strand, within ECD_01066at 1132.197 kb on - strand, within ECD_01066at 1132.275 kb on - strand, within ECD_01066at 1132.311 kb on - strand, within ECD_01066at 1132.401 kb on - strandat 1132.401 kb on - strandat 1132.411 kb on - strandat 1132.502 kb on - strand, within ECD_01067at 1132.693 kb on - strandat 1132.740 kb on - strandat 1132.776 kb on - strandat 1132.776 kb on - strandat 1132.784 kb on - strandat 1132.784 kb on - strandat 1132.786 kb on - strandat 1133.145 kb on - strand, within ECD_01068at 1133.162 kb on - strand, within ECD_01068at 1133.178 kb on - strand, within ECD_01068at 1133.290 kb on - strand, within ECD_01068at 1133.381 kb on - strandat 1133.454 kb on - strandat 1133.467 kb on + strandat 1133.500 kb on - strandat 1133.572 kb on + strandat 1133.587 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK16
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1,131,942 + +0.5
1,132,045 - ECD_01066 0.15 -0.3
1,132,125 - ECD_01066 0.34 -0.1
1,132,197 - ECD_01066 0.51 -0.4
1,132,275 - ECD_01066 0.70 -1.5
1,132,311 - ECD_01066 0.78 +0.1
1,132,401 - +0.6
1,132,401 - +1.0
1,132,411 - +0.3
1,132,502 - ECD_01067 0.33 +1.0
1,132,693 - +1.6
1,132,740 - +0.9
1,132,776 - +0.4
1,132,776 - +0.4
1,132,784 - +0.0
1,132,784 - -0.1
1,132,786 - -0.2
1,133,145 - ECD_01068 0.56 +0.5
1,133,162 - ECD_01068 0.59 -0.4
1,133,178 - ECD_01068 0.61 +0.3
1,133,290 - ECD_01068 0.78 -2.4
1,133,381 - +0.8
1,133,454 - -0.0
1,133,467 + +1.2
1,133,500 - +0.5
1,133,572 + +0.3
1,133,587 + -0.6

Or see this region's nucleotide sequence