Strain Fitness in Escherichia coli BL21 around ECD_03294

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03293 and ECD_03294 overlap by 1 nucleotidesECD_03294 and ECD_03295 are separated by 449 nucleotidesECD_03295 and ECD_03296 are separated by 37 nucleotides ECD_03293: ECD_03293 - putative Hcp1 family polymorphic toxin protein; putative colicin-like DNase/tRNase activity, at 3,445,012 to 3,446,190 _03293 ECD_03294: ECD_03294 - hypothetical protein, at 3,446,190 to 3,446,681 _03294 ECD_03295: ECD_03295 - stable heat shock chaperone, at 3,447,131 to 3,447,415 _03295 ECD_03296: ECD_03296 - gamma-glutamyltranspeptidase, at 3,447,453 to 3,449,195 _03296 Position (kb) 3446 3447Strain fitness (log2 ratio) -1 0 1at 3447.202 kb on + strand, within ECD_03295at 3447.203 kb on - strand, within ECD_03295at 3447.207 kb on + strand, within ECD_03295at 3447.234 kb on - strand, within ECD_03295at 3447.307 kb on + strand, within ECD_03295at 3447.380 kb on + strand, within ECD_03295at 3447.421 kb on + strandat 3447.454 kb on + strandat 3447.539 kb on + strandat 3447.600 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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3,447,202 + ECD_03295 0.25 -0.2
3,447,203 - ECD_03295 0.25 +0.1
3,447,207 + ECD_03295 0.27 -0.2
3,447,234 - ECD_03295 0.36 +0.3
3,447,307 + ECD_03295 0.62 +0.1
3,447,380 + ECD_03295 0.87 -0.1
3,447,421 + -0.2
3,447,454 + -0.2
3,447,539 + -0.2
3,447,600 - +0.7

Or see this region's nucleotide sequence