Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4811 and PP_4812 are separated by 305 nucleotides PP_4812 and PP_4813 are separated by 2 nucleotides PP_4813 and PP_4814 are separated by 111 nucleotides PP_4814 and PP_4815 are separated by 6 nucleotides
PP_4811: PP_4811 - Gamma-glutamyl phosphate reductase, at 5,473,523 to 5,474,794
_4811
PP_4812: PP_4812 - putative 3-methyladenine DNA glycosylase, at 5,475,100 to 5,475,768
_4812
PP_4813: PP_4813 - PAP2 family protein/DedA family protein, at 5,475,771 to 5,477,087
_4813
PP_4814: PP_4814 - ATP-dependent protease La domain protein, at 5,477,199 to 5,477,792
_4814
PP_4815: PP_4815 - putative Membrane protein, at 5,477,799 to 5,478,515
_4815
Position (kb) 5475
5476
5477
5478 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5474.771 kb on - strand at 5474.832 kb on - strand at 5474.833 kb on + strand at 5474.833 kb on + strand at 5474.834 kb on - strand at 5474.834 kb on - strand at 5474.850 kb on + strand at 5474.850 kb on + strand at 5474.850 kb on + strand at 5474.851 kb on - strand at 5474.851 kb on - strand at 5474.899 kb on + strand at 5474.899 kb on + strand at 5474.899 kb on + strand at 5474.953 kb on - strand at 5474.986 kb on - strand at 5474.986 kb on - strand at 5475.049 kb on + strand at 5475.049 kb on + strand at 5475.050 kb on - strand at 5475.050 kb on - strand at 5475.064 kb on + strand at 5475.065 kb on - strand at 5475.065 kb on - strand at 5475.229 kb on + strand, within PP_4812 at 5475.230 kb on - strand, within PP_4812 at 5475.230 kb on - strand, within PP_4812 at 5475.232 kb on + strand, within PP_4812 at 5475.249 kb on + strand, within PP_4812 at 5475.269 kb on - strand, within PP_4812 at 5475.269 kb on - strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.313 kb on + strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.314 kb on - strand, within PP_4812 at 5475.319 kb on + strand, within PP_4812 at 5475.319 kb on + strand, within PP_4812 at 5475.319 kb on + strand, within PP_4812 at 5475.319 kb on + strand, within PP_4812 at 5475.319 kb on + strand, within PP_4812 at 5475.319 kb on + strand, within PP_4812 at 5475.320 kb on - strand, within PP_4812 at 5475.320 kb on - strand, within PP_4812 at 5475.320 kb on - strand, within PP_4812 at 5475.320 kb on - strand, within PP_4812 at 5475.320 kb on - strand, within PP_4812 at 5475.322 kb on + strand, within PP_4812 at 5475.323 kb on - strand, within PP_4812 at 5475.323 kb on - strand, within PP_4812 at 5475.394 kb on + strand, within PP_4812 at 5475.512 kb on + strand, within PP_4812 at 5475.512 kb on + strand, within PP_4812 at 5475.512 kb on + strand, within PP_4812 at 5475.512 kb on + strand, within PP_4812 at 5475.513 kb on - strand, within PP_4812 at 5475.513 kb on - strand, within PP_4812 at 5475.577 kb on + strand, within PP_4812 at 5475.578 kb on - strand, within PP_4812 at 5475.685 kb on + strand, within PP_4812 at 5475.881 kb on + strand at 5475.881 kb on + strand at 5475.899 kb on - strand at 5475.899 kb on - strand at 5476.002 kb on + strand, within PP_4813 at 5476.002 kb on + strand, within PP_4813 at 5476.002 kb on + strand, within PP_4813 at 5476.003 kb on - strand, within PP_4813 at 5476.017 kb on + strand, within PP_4813 at 5476.018 kb on - strand, within PP_4813 at 5476.018 kb on - strand, within PP_4813 at 5476.018 kb on - strand, within PP_4813 at 5476.090 kb on - strand, within PP_4813 at 5476.101 kb on + strand, within PP_4813 at 5476.123 kb on + strand, within PP_4813 at 5476.123 kb on + strand, within PP_4813 at 5476.243 kb on - strand, within PP_4813 at 5476.243 kb on - strand, within PP_4813 at 5476.322 kb on - strand, within PP_4813 at 5476.322 kb on - strand, within PP_4813 at 5476.433 kb on - strand, within PP_4813 at 5476.433 kb on - strand, within PP_4813 at 5476.449 kb on + strand, within PP_4813 at 5476.449 kb on + strand, within PP_4813 at 5476.450 kb on - strand, within PP_4813 at 5476.450 kb on - strand, within PP_4813 at 5476.678 kb on + strand, within PP_4813 at 5476.678 kb on + strand, within PP_4813 at 5476.679 kb on - strand, within PP_4813 at 5476.703 kb on - strand, within PP_4813 at 5476.725 kb on + strand, within PP_4813 at 5476.725 kb on + strand, within PP_4813 at 5476.726 kb on - strand, within PP_4813 at 5476.726 kb on - strand, within PP_4813 at 5477.076 kb on + strand at 5477.076 kb on + strand at 5477.077 kb on - strand at 5477.077 kb on - strand at 5477.077 kb on - strand at 5477.082 kb on + strand at 5477.083 kb on - strand at 5477.083 kb on - strand at 5477.083 kb on - strand at 5477.106 kb on - strand at 5477.116 kb on - strand at 5477.128 kb on - strand at 5477.145 kb on + strand at 5477.151 kb on - strand at 5477.151 kb on - strand at 5477.190 kb on - strand at 5477.190 kb on - strand at 5477.317 kb on + strand, within PP_4814 at 5477.418 kb on + strand, within PP_4814 at 5477.419 kb on - strand, within PP_4814 at 5477.419 kb on - strand, within PP_4814 at 5477.762 kb on + strand at 5477.841 kb on - strand at 5478.023 kb on + strand, within PP_4815
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 5,474,771 - -0.6 5,474,832 - +2.2 5,474,833 + +1.2 5,474,833 + +2.2 5,474,834 - +2.2 5,474,834 - -0.4 5,474,850 + +2.2 5,474,850 + +0.7 5,474,850 + +1.2 5,474,851 - -0.7 5,474,851 - -0.4 5,474,899 + +0.1 5,474,899 + -0.3 5,474,899 + +0.8 5,474,953 - -0.3 5,474,986 - +1.2 5,474,986 - -0.7 5,475,049 + +0.2 5,475,049 + +0.2 5,475,050 - -1.1 5,475,050 - -0.1 5,475,064 + -0.8 5,475,065 - -0.5 5,475,065 - +1.0 5,475,229 + PP_4812 0.19 +2.2 5,475,230 - PP_4812 0.19 -0.9 5,475,230 - PP_4812 0.19 +0.3 5,475,232 + PP_4812 0.20 -0.3 5,475,249 + PP_4812 0.22 -2.0 5,475,269 - PP_4812 0.25 -0.3 5,475,269 - PP_4812 0.25 +0.4 5,475,313 + PP_4812 0.32 +0.6 5,475,313 + PP_4812 0.32 +0.1 5,475,313 + PP_4812 0.32 +0.4 5,475,313 + PP_4812 0.32 +0.7 5,475,313 + PP_4812 0.32 +2.2 5,475,313 + PP_4812 0.32 +0.7 5,475,313 + PP_4812 0.32 -0.9 5,475,313 + PP_4812 0.32 +0.2 5,475,313 + PP_4812 0.32 -1.1 5,475,314 - PP_4812 0.32 +0.3 5,475,314 - PP_4812 0.32 +1.2 5,475,314 - PP_4812 0.32 -0.9 5,475,314 - PP_4812 0.32 -0.3 5,475,314 - PP_4812 0.32 -1.1 5,475,314 - PP_4812 0.32 +0.8 5,475,314 - PP_4812 0.32 -0.3 5,475,314 - PP_4812 0.32 +2.2 5,475,314 - PP_4812 0.32 -0.5 5,475,314 - PP_4812 0.32 -2.6 5,475,314 - PP_4812 0.32 -0.1 5,475,314 - PP_4812 0.32 +0.1 5,475,314 - PP_4812 0.32 +0.5 5,475,314 - PP_4812 0.32 -0.9 5,475,314 - PP_4812 0.32 +0.7 5,475,314 - PP_4812 0.32 -1.5 5,475,319 + PP_4812 0.33 +1.4 5,475,319 + PP_4812 0.33 -0.2 5,475,319 + PP_4812 0.33 -0.3 5,475,319 + PP_4812 0.33 +1.5 5,475,319 + PP_4812 0.33 -0.2 5,475,319 + PP_4812 0.33 -0.6 5,475,320 - PP_4812 0.33 -0.5 5,475,320 - PP_4812 0.33 +0.2 5,475,320 - PP_4812 0.33 -0.8 5,475,320 - PP_4812 0.33 -0.5 5,475,320 - PP_4812 0.33 -0.4 5,475,322 + PP_4812 0.33 -1.6 5,475,323 - PP_4812 0.33 +0.4 5,475,323 - PP_4812 0.33 -3.2 5,475,394 + PP_4812 0.44 +0.4 5,475,512 + PP_4812 0.62 -1.8 5,475,512 + PP_4812 0.62 +0.0 5,475,512 + PP_4812 0.62 -0.2 5,475,512 + PP_4812 0.62 +1.4 5,475,513 - PP_4812 0.62 +0.1 5,475,513 - PP_4812 0.62 +0.0 5,475,577 + PP_4812 0.71 -0.4 5,475,578 - PP_4812 0.71 -1.9 5,475,685 + PP_4812 0.87 +0.7 5,475,881 + +0.0 5,475,881 + -0.6 5,475,899 - -0.1 5,475,899 - +0.2 5,476,002 + PP_4813 0.18 +1.8 5,476,002 + PP_4813 0.18 -0.8 5,476,002 + PP_4813 0.18 -0.7 5,476,003 - PP_4813 0.18 -1.2 5,476,017 + PP_4813 0.19 -0.4 5,476,018 - PP_4813 0.19 -1.5 5,476,018 - PP_4813 0.19 +0.8 5,476,018 - PP_4813 0.19 -1.2 5,476,090 - PP_4813 0.24 -1.9 5,476,101 + PP_4813 0.25 -0.3 5,476,123 + PP_4813 0.27 +0.2 5,476,123 + PP_4813 0.27 +0.2 5,476,243 - PP_4813 0.36 -1.6 5,476,243 - PP_4813 0.36 -0.6 5,476,322 - PP_4813 0.42 -1.5 5,476,322 - PP_4813 0.42 +0.4 5,476,433 - PP_4813 0.50 -1.6 5,476,433 - PP_4813 0.50 -1.4 5,476,449 + PP_4813 0.51 -1.6 5,476,449 + PP_4813 0.51 -0.2 5,476,450 - PP_4813 0.52 -0.5 5,476,450 - PP_4813 0.52 -1.1 5,476,678 + PP_4813 0.69 -2.2 5,476,678 + PP_4813 0.69 -0.4 5,476,679 - PP_4813 0.69 -0.6 5,476,703 - PP_4813 0.71 -1.1 5,476,725 + PP_4813 0.72 -0.6 5,476,725 + PP_4813 0.72 -3.4 5,476,726 - PP_4813 0.73 -0.9 5,476,726 - PP_4813 0.73 -0.9 5,477,076 + +0.6 5,477,076 + -0.3 5,477,077 - +0.6 5,477,077 - +1.0 5,477,077 - -1.0 5,477,082 + +0.1 5,477,083 - -0.6 5,477,083 - -1.0 5,477,083 - +1.2 5,477,106 - -0.7 5,477,116 - -0.1 5,477,128 - -0.7 5,477,145 + +1.0 5,477,151 - +2.2 5,477,151 - -0.1 5,477,190 - -0.1 5,477,190 - -0.3 5,477,317 + PP_4814 0.20 +1.1 5,477,418 + PP_4814 0.37 -0.8 5,477,419 - PP_4814 0.37 -0.3 5,477,419 - PP_4814 0.37 +0.8 5,477,762 + -0.5 5,477,841 - +2.2 5,478,023 + PP_4815 0.31 +0.2
Or see this region's nucleotide sequence