Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3754 and PP_3755 are separated by 13 nucleotides PP_3755 and PP_3756 are separated by 12 nucleotides PP_3756 and PP_3757 are separated by 48 nucleotides
PP_3754: PP_3754 - Beta-ketothiolase BktB, at 4,283,398 to 4,284,582
_3754
PP_3755: PP_3755 - 3-hydroxybutyryl-CoA dehydrogenase, at 4,284,596 to 4,285,444
_3755
PP_3756: PP_3756 - TetR family transcriptional regulator, at 4,285,457 to 4,286,038
_3756
PP_3757: PP_3757 - Chemotaxis protein CheY, at 4,286,087 to 4,286,464
_3757
Position (kb) 4284
4285
4286 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4283.689 kb on - strand, within PP_3754 at 4283.689 kb on - strand, within PP_3754 at 4283.774 kb on - strand, within PP_3754 at 4283.774 kb on - strand, within PP_3754 at 4283.831 kb on - strand, within PP_3754 at 4283.832 kb on + strand, within PP_3754 at 4283.832 kb on + strand, within PP_3754 at 4283.833 kb on - strand, within PP_3754 at 4284.236 kb on - strand at 4284.537 kb on + strand at 4284.824 kb on + strand, within PP_3755 at 4284.825 kb on - strand, within PP_3755 at 4284.946 kb on + strand, within PP_3755 at 4284.946 kb on + strand, within PP_3755 at 4284.946 kb on + strand, within PP_3755 at 4284.946 kb on + strand, within PP_3755 at 4284.947 kb on - strand, within PP_3755 at 4284.947 kb on - strand, within PP_3755 at 4284.947 kb on - strand, within PP_3755 at 4285.086 kb on - strand, within PP_3755 at 4285.362 kb on - strand at 4285.440 kb on - strand at 4285.440 kb on - strand at 4285.601 kb on + strand, within PP_3756 at 4285.601 kb on + strand, within PP_3756 at 4285.634 kb on + strand, within PP_3756 at 4285.655 kb on + strand, within PP_3756 at 4285.655 kb on + strand, within PP_3756 at 4285.655 kb on + strand, within PP_3756 at 4285.847 kb on + strand, within PP_3756 at 4285.847 kb on + strand, within PP_3756 at 4285.847 kb on + strand, within PP_3756 at 4285.924 kb on - strand, within PP_3756 at 4286.123 kb on - strand at 4286.258 kb on - strand, within PP_3757 at 4286.373 kb on + strand, within PP_3757 at 4286.374 kb on - strand at 4286.391 kb on + strand, within PP_3757 at 4286.391 kb on + strand, within PP_3757
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. remove 4,283,689 - PP_3754 0.25 -0.0 4,283,689 - PP_3754 0.25 -0.2 4,283,774 - PP_3754 0.32 -0.7 4,283,774 - PP_3754 0.32 +0.6 4,283,831 - PP_3754 0.37 -0.0 4,283,832 + PP_3754 0.37 -0.3 4,283,832 + PP_3754 0.37 +0.7 4,283,833 - PP_3754 0.37 +0.6 4,284,236 - +2.3 4,284,537 + +0.7 4,284,824 + PP_3755 0.27 -1.9 4,284,825 - PP_3755 0.27 -0.2 4,284,946 + PP_3755 0.41 +0.5 4,284,946 + PP_3755 0.41 +0.1 4,284,946 + PP_3755 0.41 -0.7 4,284,946 + PP_3755 0.41 +0.1 4,284,947 - PP_3755 0.41 +0.2 4,284,947 - PP_3755 0.41 -1.0 4,284,947 - PP_3755 0.41 +0.8 4,285,086 - PP_3755 0.58 +0.2 4,285,362 - +1.3 4,285,440 - +1.3 4,285,440 - -1.2 4,285,601 + PP_3756 0.25 +0.6 4,285,601 + PP_3756 0.25 +0.1 4,285,634 + PP_3756 0.30 +0.4 4,285,655 + PP_3756 0.34 +0.3 4,285,655 + PP_3756 0.34 +0.1 4,285,655 + PP_3756 0.34 +1.5 4,285,847 + PP_3756 0.67 +0.3 4,285,847 + PP_3756 0.67 +0.6 4,285,847 + PP_3756 0.67 +1.1 4,285,924 - PP_3756 0.80 +3.3 4,286,123 - +1.8 4,286,258 - PP_3757 0.45 +0.5 4,286,373 + PP_3757 0.76 -0.5 4,286,374 - +0.7 4,286,391 + PP_3757 0.80 +1.2 4,286,391 + PP_3757 0.80 -0.1
Or see this region's nucleotide sequence