Strain Fitness in Pseudomonas putida KT2440 around PP_0117

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
122,225	+				+0.1
122,225	+				-0.5
122,225	+				+0.6
122,237	+				+0.2
122,252	+				+0.0
122,253	-				+0.1
122,404	+		PP_0116	0.22	+0.2
122,421	-		PP_0116	0.24	-0.7
122,421	-		PP_0116	0.24	+0.3
122,499	+		PP_0116	0.35	+0.8
122,500	-		PP_0116	0.35	-1.4
122,707	-		PP_0116	0.64	+1.2
122,707	-		PP_0116	0.64	-2.5
122,717	+		PP_0116	0.66	-0.6
122,718	-		PP_0116	0.66	+0.8
122,718	-		PP_0116	0.66	-0.3
122,718	-		PP_0116	0.66	+0.5
122,741	+		PP_0116	0.69	+1.5
122,741	+		PP_0116	0.69	+1.8
122,973	-				+2.2
122,997	+				-0.4
122,998	-				+0.2
122,998	-				+0.6
123,030	+				+0.7
123,030	+				-1.1
123,031	-				-1.5
123,118	+				-2.5
123,118	+				-2.6
123,119	-				+0.6
123,119	-				-2.9
123,291	+		PP_0117	0.26	-1.5
123,292	-		PP_0117	0.26	-1.9
123,331	+		PP_0117	0.31	-1.4
123,331	+		PP_0117	0.31	-1.5
123,527	+		PP_0117	0.56	-2.4
123,527	+		PP_0117	0.56	-1.2
123,527	+		PP_0117	0.56	-1.8
123,527	+		PP_0117	0.56	-2.4
123,527	+		PP_0117	0.56	-2.0
123,528	-		PP_0117	0.57	-1.9
123,528	-		PP_0117	0.57	-3.0
123,788	-		PP_0117	0.90	-0.2
123,788	-		PP_0117	0.90	-1.7
123,951	+		PP_0118	0.12	+0.7
123,951	+		PP_0118	0.12	-1.0
123,951	+		PP_0118	0.12	-0.2
123,951	+		PP_0118	0.12	-0.2
123,952	-		PP_0118	0.12	-0.6
123,952	-		PP_0118	0.12	-1.2
123,952	-		PP_0118	0.12	-0.6
123,952	-		PP_0118	0.12	+0.2
123,952	-		PP_0118	0.12	+1.0
124,152	+		PP_0118	0.38	-3.5
124,152	+		PP_0118	0.38	-0.8
124,153	-		PP_0118	0.38	-3.3
124,153	-		PP_0118	0.38	-3.1
124,153	-		PP_0118	0.38	-2.7
124,187	+		PP_0118	0.42	-3.1
124,187	+		PP_0118	0.42	-1.7
124,188	-		PP_0118	0.42	+2.2
124,271	-		PP_0118	0.53	-3.0
124,271	-		PP_0118	0.53	-2.2
124,343	+		PP_0118	0.62	-2.3
124,344	-		PP_0118	0.63	-1.5
124,419	+		PP_0118	0.72	-1.4
124,419	+		PP_0118	0.72	-1.3
124,419	+		PP_0118	0.72	-3.0
124,420	-		PP_0118	0.72	-2.3
124,448	+		PP_0118	0.76	-2.8
124,482	-		PP_0118	0.80	-2.9
124,482	-		PP_0118	0.80	-1.1
124,650	+				-1.0
124,651	-				-0.4
124,853	-		PP_0119	0.46	-0.8