Strain Fitness in Escherichia coli BW25113 around b4598

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydcW and yncL are separated by 96 nucleotidesyncL and b1445 are separated by 194 nucleotidesb1445 and ydcY are separated by 85 nucleotidesydcY and ydcZ are separated by 0 nucleotides b1444: ydcW - medium chain aldehyde dehydrogenase (NCBI), at 1,513,602 to 1,515,026 ydcW b4598: yncL - hypothetical protein (RefSeq), at 1,515,123 to 1,515,218 yncL b1445: b1445 - orf, hypothetical protein (VIMSS), at 1,515,413 to 1,515,586 b1445 b1446: ydcY - hypothetical protein (NCBI), at 1,515,672 to 1,515,905 ydcY b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355 ydcZ Position (kb) 1515 1516Strain fitness (log2 ratio) -1 0 1at 1514.310 kb on - strand, within ydcWat 1514.401 kb on + strand, within ydcWat 1514.401 kb on + strand, within ydcWat 1514.435 kb on + strand, within ydcWat 1514.503 kb on + strand, within ydcWat 1514.625 kb on + strand, within ydcWat 1514.706 kb on + strand, within ydcWat 1514.920 kb on + strandat 1515.090 kb on + strandat 1515.199 kb on - strand, within yncLat 1515.247 kb on + strandat 1515.249 kb on + strandat 1515.252 kb on - strandat 1515.257 kb on - strandat 1515.257 kb on - strandat 1515.259 kb on + strandat 1515.608 kb on + strandat 1515.670 kb on - strandat 1515.791 kb on + strand, within ydcYat 1515.839 kb on - strand, within ydcYat 1515.839 kb on - strand, within ydcYat 1515.892 kb on + strandat 1515.892 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,514,310 - ydcW b1444 0.50 +0.7
1,514,401 + ydcW b1444 0.56 -0.2
1,514,401 + ydcW b1444 0.56 +0.6
1,514,435 + ydcW b1444 0.58 +0.6
1,514,503 + ydcW b1444 0.63 -0.4
1,514,625 + ydcW b1444 0.72 -1.3
1,514,706 + ydcW b1444 0.77 +0.3
1,514,920 + +0.0
1,515,090 + -0.3
1,515,199 - yncL b4598 0.79 -1.0
1,515,247 + -1.3
1,515,249 + -0.5
1,515,252 - -0.6
1,515,257 - -0.3
1,515,257 - +0.1
1,515,259 + +0.4
1,515,608 + -0.3
1,515,670 - +0.3
1,515,791 + ydcY b1446 0.51 -1.7
1,515,839 - ydcY b1446 0.71 +0.6
1,515,839 - ydcY b1446 0.71 +0.3
1,515,892 + +0.9
1,515,892 + -1.5

Or see this region's nucleotide sequence