Strain Fitness in Escherichia coli BW25113 around b3849

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Aerobic with Hygromycin B 0.000015625 mM
4,030,280	+	pepQ	b3847	0.82	+0.7
4,030,293	+	pepQ	b3847	0.83	-0.2
4,030,298	+	pepQ	b3847	0.84	+0.7
4,030,314	-	pepQ	b3847	0.85	+0.4
4,030,328	+	pepQ	b3847	0.86	+0.4
4,030,329	-	pepQ	b3847	0.86	+0.0
4,030,329	-	pepQ	b3847	0.86	-1.0
4,030,329	-	pepQ	b3847	0.86	-0.2
4,030,336	-	pepQ	b3847	0.86	+0.9
4,030,336	-	pepQ	b3847	0.86	+0.2
4,030,338	+	pepQ	b3847	0.87	+0.7
4,030,350	-	pepQ	b3847	0.88	+0.5
4,030,384	-				+0.6
4,030,394	-				+0.7
4,030,445	+				+0.2
4,030,467	-				-0.9
4,030,467	-				+0.2
4,030,579	+	yigZ	b3848	0.10	-0.1
4,030,642	+	yigZ	b3848	0.21	+0.7
4,030,642	+	yigZ	b3848	0.21	-0.1
4,030,647	-	yigZ	b3848	0.21	+0.3
4,030,664	+	yigZ	b3848	0.24	-0.5
4,030,666	+	yigZ	b3848	0.25	+0.5
4,030,670	-	yigZ	b3848	0.25	-0.1
4,030,670	-	yigZ	b3848	0.25	-0.5
4,030,670	-	yigZ	b3848	0.25	+0.7
4,030,672	-	yigZ	b3848	0.26	-0.8
4,030,672	-	yigZ	b3848	0.26	+0.5
4,030,682	+	yigZ	b3848	0.27	+0.7
4,030,690	-	yigZ	b3848	0.28	-1.1
4,030,702	+	yigZ	b3848	0.30	+0.0
4,030,806	+	yigZ	b3848	0.47	+0.6
4,030,806	+	yigZ	b3848	0.47	-0.3
4,030,812	-	yigZ	b3848	0.48	-0.3
4,030,836	-	yigZ	b3848	0.52	-0.4
4,030,836	-	yigZ	b3848	0.52	+1.2
4,030,836	-	yigZ	b3848	0.52	+1.2
4,030,836	-	yigZ	b3848	0.52	+0.7
4,030,843	-	yigZ	b3848	0.53	+0.2
4,030,843	-	yigZ	b3848	0.53	-0.1
4,030,848	-	yigZ	b3848	0.54	-0.3
4,030,848	-	yigZ	b3848	0.54	-0.2
4,030,900	+	yigZ	b3848	0.63	+0.2
4,030,900	+	yigZ	b3848	0.63	-0.1
4,030,924	-	yigZ	b3848	0.67	+0.2
4,030,925	-	yigZ	b3848	0.67	-0.3
4,030,932	+	yigZ	b3848	0.68	+0.5
4,030,948	-	yigZ	b3848	0.70	-0.4
4,030,955	+	yigZ	b3848	0.72	+0.5
4,030,956	-	yigZ	b3848	0.72	-0.5
4,030,988	-	yigZ	b3848	0.77	+1.0
4,031,038	-	yigZ	b3848	0.85	+0.6
4,031,162	+				-0.3
4,031,162	+				-0.4
4,031,189	-				+0.2
4,031,204	-				-1.4
4,031,211	+				-0.8
4,031,226	+				+0.2
4,031,226	+				-0.3
4,031,241	+				-1.2
4,031,241	+				+0.2
4,031,254	+				+1.6
4,031,260	-				-1.7
4,031,260	-				-2.3
4,031,280	+				-1.2
4,031,285	+				+0.2
4,031,310	+				+0.7
4,031,321	+	trkH	b3849	0.11	-2.0
4,031,326	+	trkH	b3849	0.11	-0.4
4,031,329	-	trkH	b3849	0.11	-1.2
4,031,357	+	trkH	b3849	0.13	-1.3
4,031,374	-	trkH	b3849	0.14	-3.0
4,031,384	-	trkH	b3849	0.15	-0.4
4,031,392	+	trkH	b3849	0.15	-1.4
4,031,392	+	trkH	b3849	0.15	-0.6
4,031,400	-	trkH	b3849	0.16	-1.2
4,031,400	-	trkH	b3849	0.16	-0.3
4,031,402	+	trkH	b3849	0.16	-1.2
4,031,402	+	trkH	b3849	0.16	-0.8
4,031,402	+	trkH	b3849	0.16	-1.7
4,031,410	-	trkH	b3849	0.17	+1.2
4,031,410	-	trkH	b3849	0.17	+0.5
4,031,410	-	trkH	b3849	0.17	-0.7
4,031,410	-	trkH	b3849	0.17	+0.2
4,031,410	-	trkH	b3849	0.17	-1.2
4,031,412	+	trkH	b3849	0.17	-1.8
4,031,429	+	trkH	b3849	0.18	-0.2
4,031,429	+	trkH	b3849	0.18	+1.5
4,031,445	+	trkH	b3849	0.19	-0.7
4,031,492	-	trkH	b3849	0.22	+0.6
4,031,492	-	trkH	b3849	0.22	+0.2
4,031,494	+	trkH	b3849	0.22	-2.8
4,031,497	-	trkH	b3849	0.23	-0.7
4,031,499	+	trkH	b3849	0.23	-0.6
4,031,507	-	trkH	b3849	0.23	-0.4
4,031,513	+	trkH	b3849	0.24	+1.2
4,031,522	-	trkH	b3849	0.24	+0.2
4,031,527	+	trkH	b3849	0.25	-0.8
4,031,527	+	trkH	b3849	0.25	+0.2
4,031,531	-	trkH	b3849	0.25	-0.4
4,031,531	-	trkH	b3849	0.25	+1.2
4,031,544	-	trkH	b3849	0.26	-2.3
4,031,544	-	trkH	b3849	0.26	-1.7
4,031,575	-	trkH	b3849	0.28	-1.8
4,031,575	-	trkH	b3849	0.28	-0.4
4,031,587	-	trkH	b3849	0.29	-0.7
4,031,587	-	trkH	b3849	0.29	+0.2
4,031,636	-	trkH	b3849	0.32	-0.8
4,031,636	-	trkH	b3849	0.32	-1.5
4,031,637	+	trkH	b3849	0.32	-1.4
4,031,639	+	trkH	b3849	0.32	+0.6
4,031,642	+	trkH	b3849	0.33	-1.2
4,031,652	-	trkH	b3849	0.33	-2.2
4,031,728	+	trkH	b3849	0.39	-1.2
4,031,728	+	trkH	b3849	0.39	-2.2
4,031,787	+	trkH	b3849	0.43	-0.8
4,031,816	-	trkH	b3849	0.45	-0.9
4,031,857	-	trkH	b3849	0.47	-0.4
4,031,884	-	trkH	b3849	0.49	-1.8
4,031,886	-	trkH	b3849	0.49	-1.1
4,031,886	-	trkH	b3849	0.49	+0.5
4,031,944	+	trkH	b3849	0.53	-0.7
4,032,035	+	trkH	b3849	0.60	-1.4
4,032,056	+	trkH	b3849	0.61	-1.0
4,032,064	-	trkH	b3849	0.62	-1.3
4,032,064	-	trkH	b3849	0.62	+0.5
4,032,078	+	trkH	b3849	0.63	-0.7
4,032,078	+	trkH	b3849	0.63	-0.2
4,032,102	-	trkH	b3849	0.64	-2.4
4,032,136	+	trkH	b3849	0.67	-1.4
4,032,142	-	trkH	b3849	0.67	-1.0
4,032,245	-	trkH	b3849	0.74	-1.0
4,032,253	+	trkH	b3849	0.75	-1.2
4,032,287	+	trkH	b3849	0.77	+0.2
4,032,287	+	trkH	b3849	0.77	+0.6
4,032,298	-	trkH	b3849	0.78	-0.8
4,032,340	-	trkH	b3849	0.81	-0.3
4,032,386	-	trkH	b3849	0.84	-0.5
4,032,421	+	trkH	b3849	0.86	-2.3
4,032,425	-	trkH	b3849	0.87	+0.8
4,032,425	-	trkH	b3849	0.87	-1.0
4,032,433	+	trkH	b3849	0.87	-1.4
4,032,433	+	trkH	b3849	0.87	-0.2
4,032,452	+	trkH	b3849	0.88	-0.2
4,032,505	-				-0.8
4,032,505	-				-0.4
4,032,509	+				-1.0
4,032,521	-				+1.9
4,032,592	-				+1.2
4,032,604	+				-1.4
4,032,614	+				-0.0
4,032,622	+				+0.5
4,032,624	-				-0.0
4,032,960	-	hemG	b3850	0.60	+1.2