Strain Fitness in Escherichia coli BW25113 around b4557

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpmH and rnpA are separated by 16 nucleotidesrnpA and yidD overlap by 37 nucleotidesyidD and yidC are separated by 2 nucleotides b3703: rpmH - 50S ribosomal protein L34 (NCBI), at 3,882,359 to 3,882,499 rpmH b3704: rnpA - ribonuclease P (NCBI), at 3,882,516 to 3,882,875 rnpA b4557: yidD - hypothetical protein (NCBI), at 3,882,839 to 3,883,096 yidD b3705: yidC - inner membrane protein translocase component YidC (NCBI), at 3,883,099 to 3,884,745 yidC Position (kb) 3882 3883 3884Strain fitness (log2 ratio) -2 -1 0 1 2at 3881.847 kb on - strandat 3881.847 kb on - strandat 3881.884 kb on - strandat 3881.914 kb on + strandat 3881.932 kb on - strandat 3881.959 kb on + strandat 3881.972 kb on - strandat 3881.976 kb on + strandat 3882.000 kb on - strandat 3882.000 kb on - strandat 3882.009 kb on - strandat 3882.088 kb on + strandat 3882.097 kb on - strandat 3882.110 kb on + strandat 3882.110 kb on + strandat 3882.118 kb on - strandat 3882.118 kb on - strandat 3882.120 kb on - strandat 3882.163 kb on + strandat 3882.172 kb on - strandat 3882.876 kb on - strand, within yidDat 3882.876 kb on - strand, within yidDat 3882.883 kb on + strand, within yidDat 3882.901 kb on + strand, within yidDat 3882.901 kb on - strand, within yidDat 3882.902 kb on - strand, within yidDat 3882.941 kb on - strand, within yidDat 3882.960 kb on + strand, within yidDat 3882.978 kb on + strand, within yidDat 3883.034 kb on + strand, within yidDat 3883.034 kb on + strand, within yidD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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3,881,847 - -0.5
3,881,847 - +2.2
3,881,884 - -0.0
3,881,914 + +0.8
3,881,932 - +0.6
3,881,959 + -0.6
3,881,972 - -0.0
3,881,976 + -0.4
3,882,000 - +0.2
3,882,000 - +0.2
3,882,009 - +0.5
3,882,088 + -0.8
3,882,097 - -0.6
3,882,110 + +0.7
3,882,110 + -0.4
3,882,118 - +0.2
3,882,118 - -0.8
3,882,120 - -1.0
3,882,163 + -0.2
3,882,172 - +2.2
3,882,876 - yidD b4557 0.14 +0.8
3,882,876 - yidD b4557 0.14 -1.1
3,882,883 + yidD b4557 0.17 -0.9
3,882,901 + yidD b4557 0.24 +0.6
3,882,901 - yidD b4557 0.24 -0.4
3,882,902 - yidD b4557 0.24 +0.2
3,882,941 - yidD b4557 0.40 +1.2
3,882,960 + yidD b4557 0.47 -0.7
3,882,978 + yidD b4557 0.54 -2.1
3,883,034 + yidD b4557 0.76 -1.6
3,883,034 + yidD b4557 0.76 -1.4

Or see this region's nucleotide sequence