Strain Fitness in Escherichia coli BW25113 around b2189

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyejM and proL are separated by 74 nucleotidesproL and yejO are separated by 102 nucleotides b2188: yejM - predicted hydrolase, inner membrane (NCBI), at 2,282,398 to 2,284,158 yejM b2189: proL - tRNA-Pro (NCBI), at 2,284,233 to 2,284,309 proL b2190: yejO - predicted autotransporter outer membrane protein (RefSeq), at 2,284,412 to 2,286,936 yejO Position (kb) 2284 2285Strain fitness (log2 ratio) -2 -1 0 1 2at 2283.279 kb on + strand, within yejMat 2283.304 kb on - strand, within yejMat 2283.324 kb on + strand, within yejMat 2283.324 kb on + strand, within yejMat 2283.395 kb on + strand, within yejMat 2283.395 kb on + strand, within yejMat 2283.409 kb on - strand, within yejMat 2283.474 kb on - strand, within yejMat 2283.688 kb on + strand, within yejMat 2283.688 kb on + strand, within yejMat 2283.712 kb on - strand, within yejMat 2283.752 kb on + strand, within yejMat 2283.752 kb on + strand, within yejMat 2283.752 kb on + strand, within yejMat 2283.758 kb on - strand, within yejMat 2283.760 kb on - strand, within yejMat 2283.789 kb on + strand, within yejMat 2283.797 kb on + strand, within yejMat 2283.875 kb on + strand, within yejMat 2283.875 kb on + strand, within yejMat 2283.900 kb on - strand, within yejMat 2283.910 kb on - strand, within yejMat 2283.917 kb on - strand, within yejMat 2283.980 kb on - strand, within yejMat 2284.005 kb on + strandat 2284.005 kb on + strandat 2284.005 kb on + strandat 2284.051 kb on - strandat 2284.051 kb on - strandat 2284.056 kb on - strandat 2284.205 kb on + strandat 2284.255 kb on - strand, within proLat 2284.384 kb on - strandat 2284.395 kb on - strandat 2284.454 kb on + strandat 2284.454 kb on + strandat 2284.462 kb on - strandat 2284.479 kb on - strandat 2284.506 kb on + strandat 2284.528 kb on - strandat 2284.528 kb on - strandat 2284.606 kb on - strandat 2284.606 kb on - strandat 2284.667 kb on + strand, within yejOat 2284.704 kb on + strand, within yejOat 2284.729 kb on - strand, within yejOat 2284.729 kb on - strand, within yejOat 2284.748 kb on - strand, within yejOat 2284.755 kb on - strand, within yejOat 2284.764 kb on - strand, within yejOat 2284.764 kb on - strand, within yejOat 2284.795 kb on - strand, within yejOat 2284.795 kb on - strand, within yejOat 2284.796 kb on - strand, within yejOat 2284.796 kb on - strand, within yejOat 2284.932 kb on + strand, within yejOat 2284.982 kb on - strand, within yejOat 2284.982 kb on - strand, within yejOat 2284.993 kb on + strand, within yejOat 2285.000 kb on + strand, within yejOat 2285.001 kb on - strand, within yejOat 2285.126 kb on + strand, within yejOat 2285.258 kb on - strand, within yejOat 2285.265 kb on - strandat 2285.265 kb on - strand, within yejO

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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2,283,279 + yejM b2188 0.50 -0.2
2,283,304 - yejM b2188 0.51 -0.7
2,283,324 + yejM b2188 0.53 -0.3
2,283,324 + yejM b2188 0.53 -0.2
2,283,395 + yejM b2188 0.57 +0.4
2,283,395 + yejM b2188 0.57 -1.0
2,283,409 - yejM b2188 0.57 -0.6
2,283,474 - yejM b2188 0.61 +1.0
2,283,688 + yejM b2188 0.73 +0.4
2,283,688 + yejM b2188 0.73 -0.2
2,283,712 - yejM b2188 0.75 -0.9
2,283,752 + yejM b2188 0.77 -0.0
2,283,752 + yejM b2188 0.77 -0.2
2,283,752 + yejM b2188 0.77 +0.4
2,283,758 - yejM b2188 0.77 -0.9
2,283,760 - yejM b2188 0.77 -1.2
2,283,789 + yejM b2188 0.79 +0.2
2,283,797 + yejM b2188 0.79 -1.2
2,283,875 + yejM b2188 0.84 -0.3
2,283,875 + yejM b2188 0.84 -0.5
2,283,900 - yejM b2188 0.85 +0.6
2,283,910 - yejM b2188 0.86 +1.4
2,283,917 - yejM b2188 0.86 +0.3
2,283,980 - yejM b2188 0.90 -0.3
2,284,005 + -0.5
2,284,005 + +0.1
2,284,005 + +0.5
2,284,051 - +1.4
2,284,051 - -0.6
2,284,056 - -0.2
2,284,205 + +0.4
2,284,255 - proL b2189 0.29 -0.7
2,284,384 - -0.5
2,284,395 - -0.9
2,284,454 + -0.1
2,284,454 + -1.2
2,284,462 - -0.1
2,284,479 - -0.1
2,284,506 + -2.0
2,284,528 - -0.3
2,284,528 - -0.5
2,284,606 - +0.4
2,284,606 - +2.0
2,284,667 + yejO b2190 0.10 +0.6
2,284,704 + yejO b2190 0.12 -1.0
2,284,729 - yejO b2190 0.13 +0.3
2,284,729 - yejO b2190 0.13 -1.5
2,284,748 - yejO b2190 0.13 -0.3
2,284,755 - yejO b2190 0.14 +0.2
2,284,764 - yejO b2190 0.14 -1.4
2,284,764 - yejO b2190 0.14 -0.6
2,284,795 - yejO b2190 0.15 +0.0
2,284,795 - yejO b2190 0.15 -0.1
2,284,796 - yejO b2190 0.15 +0.4
2,284,796 - yejO b2190 0.15 -0.3
2,284,932 + yejO b2190 0.21 +0.4
2,284,982 - yejO b2190 0.23 +1.8
2,284,982 - yejO b2190 0.23 -0.3
2,284,993 + yejO b2190 0.23 +0.3
2,285,000 + yejO b2190 0.23 -0.2
2,285,001 - yejO b2190 0.23 +0.0
2,285,126 + yejO b2190 0.28 -0.0
2,285,258 - yejO b2190 0.34 +0.8
2,285,265 - +0.4
2,285,265 - yejO b2190 0.34 -1.6

Or see this region's nucleotide sequence