Experiment: LB Aerobic with Hygromycin B 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegK and yegL overlap by 4 nucleotides yegL and ryeC are separated by 180 nucleotides ryeC and ibsA overlap by 103 nucleotides ibsA and ryeD are separated by 235 nucleotides ryeD and ibsB overlap by 99 nucleotides ibsB and b2074 are separated by 278 nucleotides
b2072: yegK - hypothetical protein (NCBI), at 2,149,735 to 2,150,496
yegK
b2073: yegL - hypothetical protein (NCBI), at 2,150,493 to 2,151,152
yegL
b4436: ryeC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq), at 2,151,333 to 2,151,475
ryeC
b4667: ibsA - toxic membrane protein (RefSeq), at 2,151,373 to 2,151,432
ibsA
b4437: ryeD - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 2,151,668 to 2,151,803
ryeD
b4668: ibsB - toxic membrane protein (RefSeq), at 2,151,705 to 2,151,761
ibsB
b2074: b2074 - putative membrane protein (VIMSS), at 2,152,040 to 2,153,287
b2074
Position (kb)
2151
2152 Strain fitness (log2 ratio)
-2
-1
0
1 at 2150.338 kb on - strand, within yegK at 2150.633 kb on + strand, within yegL at 2150.650 kb on + strand, within yegL at 2150.753 kb on + strand, within yegL at 2150.819 kb on - strand, within yegL at 2150.819 kb on - strand, within yegL at 2150.836 kb on + strand, within yegL at 2150.836 kb on + strand, within yegL at 2150.846 kb on - strand, within yegL at 2150.846 kb on - strand, within yegL at 2150.856 kb on + strand, within yegL at 2150.863 kb on + strand, within yegL at 2150.865 kb on + strand, within yegL at 2150.891 kb on - strand, within yegL at 2150.919 kb on + strand, within yegL at 2150.919 kb on + strand, within yegL at 2150.943 kb on - strand, within yegL at 2150.943 kb on - strand, within yegL at 2150.962 kb on - strand, within yegL at 2151.035 kb on + strand, within yegL at 2151.046 kb on - strand, within yegL at 2151.104 kb on - strand at 2151.104 kb on - strand at 2151.195 kb on - strand at 2151.277 kb on + strand at 2151.299 kb on - strand at 2151.311 kb on - strand at 2151.339 kb on + strand at 2151.339 kb on - strand at 2151.354 kb on + strand, within ryeC at 2151.354 kb on + strand, within ryeC at 2151.388 kb on - strand at 2151.411 kb on + strand at 2151.411 kb on + strand at 2151.432 kb on - strand at 2151.512 kb on - strand at 2151.671 kb on + strand at 2151.747 kb on + strand at 2151.747 kb on + strand at 2152.197 kb on + strand, within b2074 at 2152.228 kb on + strand, within b2074 at 2152.253 kb on + strand, within b2074 at 2152.447 kb on + strand, within b2074
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM remove 2,150,338 - yegK b2072 0.79 +0.0 2,150,633 + yegL b2073 0.21 -0.2 2,150,650 + yegL b2073 0.24 -1.4 2,150,753 + yegL b2073 0.39 -0.2 2,150,819 - yegL b2073 0.49 +0.5 2,150,819 - yegL b2073 0.49 +0.1 2,150,836 + yegL b2073 0.52 +0.7 2,150,836 + yegL b2073 0.52 +1.0 2,150,846 - yegL b2073 0.53 +0.5 2,150,846 - yegL b2073 0.53 -2.0 2,150,856 + yegL b2073 0.55 +0.2 2,150,863 + yegL b2073 0.56 -0.6 2,150,865 + yegL b2073 0.56 -0.4 2,150,891 - yegL b2073 0.60 +0.5 2,150,919 + yegL b2073 0.65 -0.1 2,150,919 + yegL b2073 0.65 -0.5 2,150,943 - yegL b2073 0.68 -0.6 2,150,943 - yegL b2073 0.68 +0.7 2,150,962 - yegL b2073 0.71 +0.6 2,151,035 + yegL b2073 0.82 +0.2 2,151,046 - yegL b2073 0.84 +0.7 2,151,104 - +0.1 2,151,104 - +0.4 2,151,195 - +0.0 2,151,277 + +1.0 2,151,299 - -0.5 2,151,311 - -0.3 2,151,339 + +0.1 2,151,339 - +0.2 2,151,354 + ryeC b4436 0.15 -0.8 2,151,354 + ryeC b4436 0.15 -0.2 2,151,388 - -0.2 2,151,411 + +0.6 2,151,411 + +0.4 2,151,432 - +1.4 2,151,512 - -0.6 2,151,671 + +0.4 2,151,747 + -0.0 2,151,747 + -0.4 2,152,197 + b2074 b2074 0.13 +0.5 2,152,228 + b2074 b2074 0.15 -0.4 2,152,253 + b2074 b2074 0.17 -0.9 2,152,447 + b2074 b2074 0.33 +0.6
Or see this region's nucleotide sequence