Strain Fitness in Escherichia coli BW25113 around b1231

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrttR and tpr overlap by 150 nucleotidestpr and tyrV are separated by 67 nucleotidestyrV and tyrT are separated by 209 nucleotidestyrT and purU are separated by 159 nucleotides b4425: rttR - regulatory sRNA (NCBI), at 1,286,289 to 1,286,459 rttR b1229: tpr - predicted protamine-like protein (RefSeq), at 1,286,310 to 1,286,399 tpr b1230: tyrV - tRNA-Tyr (NCBI), at 1,286,467 to 1,286,551 tyrV b1231: tyrT - tRNA-Tyr (NCBI), at 1,286,761 to 1,286,845 tyrT b1232: purU - formyltetrahydrofolate deformylase (NCBI), at 1,287,005 to 1,287,847 purU Position (kb) 1286 1287Strain fitness (log2 ratio) -1 0 1at 1286.125 kb on + strandat 1286.316 kb on - strandat 1286.316 kb on - strandat 1286.690 kb on - strandat 1286.690 kb on - strandat 1286.805 kb on + strand, within tyrTat 1286.916 kb on + strandat 1287.056 kb on + strandat 1287.064 kb on + strandat 1287.233 kb on + strand, within purUat 1287.255 kb on - strand, within purUat 1287.255 kb on - strand, within purUat 1287.342 kb on + strand, within purUat 1287.375 kb on + strand, within purUat 1287.423 kb on - strand, within purUat 1287.425 kb on + strand, within purUat 1287.439 kb on + strand, within purUat 1287.448 kb on + strand, within purUat 1287.473 kb on - strand, within purUat 1287.491 kb on + strand, within purUat 1287.647 kb on - strand, within purUat 1287.691 kb on - strand, within purUat 1287.714 kb on + strand, within purUat 1287.801 kb on - strandat 1287.801 kb on - strandat 1287.837 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,286,125 + -0.1
1,286,316 - +0.3
1,286,316 - -0.7
1,286,690 - -0.1
1,286,690 - -0.8
1,286,805 + tyrT b1231 0.52 -0.7
1,286,916 + -0.8
1,287,056 + +0.0
1,287,064 + -0.3
1,287,233 + purU b1232 0.27 +0.5
1,287,255 - purU b1232 0.30 +0.7
1,287,255 - purU b1232 0.30 +0.1
1,287,342 + purU b1232 0.40 -1.5
1,287,375 + purU b1232 0.44 +0.3
1,287,423 - purU b1232 0.50 +0.3
1,287,425 + purU b1232 0.50 +1.3
1,287,439 + purU b1232 0.51 -0.5
1,287,448 + purU b1232 0.53 +0.7
1,287,473 - purU b1232 0.56 +0.2
1,287,491 + purU b1232 0.58 +0.5
1,287,647 - purU b1232 0.76 +0.4
1,287,691 - purU b1232 0.81 -0.2
1,287,714 + purU b1232 0.84 -0.2
1,287,801 - +0.3
1,287,801 - +0.6
1,287,837 + +0.8

Or see this region's nucleotide sequence