Strain Fitness in Escherichia coli BW25113 around b4175

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Aerobic with Hygromycin B 0.000015625 mM
4,400,356	+	hflK	b4174	0.23	-2.7
4,400,385	-	hflK	b4174	0.26	-0.9
4,400,385	-	hflK	b4174	0.26	-1.7
4,400,411	+	hflK	b4174	0.28	-1.1
4,400,416	+	hflK	b4174	0.28	+0.1
4,400,416	+	hflK	b4174	0.28	-1.9
4,400,429	-	hflK	b4174	0.29	-2.9
4,400,476	-	hflK	b4174	0.33	-2.5
4,400,502	-	hflK	b4174	0.35	+1.1
4,400,534	-	hflK	b4174	0.38	-0.7
4,400,546	-	hflK	b4174	0.38	-1.9
4,400,555	-	hflK	b4174	0.39	-3.9
4,400,582	+	hflK	b4174	0.41	-1.9
4,400,586	+	hflK	b4174	0.42	-2.7
4,400,586	+	hflK	b4174	0.42	-3.0
4,400,620	-	hflK	b4174	0.44	-1.9
4,400,643	+	hflK	b4174	0.46	-2.6
4,400,654	+	hflK	b4174	0.47	-2.9
4,400,704	-	hflK	b4174	0.51	-0.6
4,400,704	-	hflK	b4174	0.51	-2.1
4,400,709	-	hflK	b4174	0.51	-1.3
4,400,716	+	hflK	b4174	0.52	-2.7
4,400,716	+	hflK	b4174	0.52	-1.5
4,400,747	+	hflK	b4174	0.54	-1.8
4,400,749	+	hflK	b4174	0.55	-3.3
4,400,767	-	hflK	b4174	0.56	-1.3
4,400,779	-	hflK	b4174	0.57	-2.4
4,400,779	-	hflK	b4174	0.57	+0.1
4,400,860	+	hflK	b4174	0.63	-3.5
4,400,875	-	hflK	b4174	0.65	-1.4
4,400,908	-	hflK	b4174	0.67	+1.1
4,400,932	+	hflK	b4174	0.69	-2.6
4,400,933	+	hflK	b4174	0.69	-0.5
4,400,942	+	hflK	b4174	0.70	+0.1
4,400,942	+	hflK	b4174	0.70	+0.1
4,400,942	-	hflK	b4174	0.70	-2.1
4,400,951	-	hflK	b4174	0.71	-0.9
4,400,951	-	hflK	b4174	0.71	-0.9
4,400,952	+	hflK	b4174	0.71	-1.9
4,400,952	+	hflK	b4174	0.71	-2.4
4,400,952	+	hflK	b4174	0.71	-1.9
4,400,966	+	hflK	b4174	0.72	-1.9
4,400,983	+	hflK	b4174	0.73	-2.3
4,400,991	-	hflK	b4174	0.74	-2.4
4,401,008	+	hflK	b4174	0.75	-2.1
4,401,010	-	hflK	b4174	0.75	-1.1
4,401,073	-	hflK	b4174	0.80	-2.4
4,401,091	-	hflK	b4174	0.82	-2.5
4,401,091	-	hflK	b4174	0.82	-0.9
4,401,218	+				-1.6
4,401,238	-				-0.9
4,401,238	-				-1.8
4,401,252	-				-1.3
4,401,259	+				+0.3
4,401,310	+				-0.0
4,401,362	+				-2.6
4,401,368	+				-2.5
4,401,368	+				-2.3
4,401,408	+				-2.4
4,401,408	+				-2.6
4,401,408	+				-0.9
4,401,408	+				-2.5
4,401,426	+	hflC	b4175	0.10	-2.5
4,401,436	+	hflC	b4175	0.11	-1.7
4,401,467	-	hflC	b4175	0.14	-1.5
4,401,526	+	hflC	b4175	0.20	-2.1
4,401,605	-	hflC	b4175	0.28	-2.3
4,401,628	+	hflC	b4175	0.30	-2.3
4,401,639	-	hflC	b4175	0.31	-3.3
4,401,643	-	hflC	b4175	0.32	-2.8
4,401,756	-	hflC	b4175	0.43	-3.3
4,401,764	-	hflC	b4175	0.44	-0.2
4,401,764	-	hflC	b4175	0.44	-1.3
4,401,792	-	hflC	b4175	0.47	-1.7
4,401,792	-	hflC	b4175	0.47	-1.3
4,401,910	+	hflC	b4175	0.58	-3.7
4,401,912	+	hflC	b4175	0.59	-3.2
4,401,912	+	hflC	b4175	0.59	-3.9
4,401,912	+	hflC	b4175	0.59	-1.3
4,401,912	+	hflC	b4175	0.59	-0.5
4,401,920	-	hflC	b4175	0.59	-0.9
4,401,920	-	hflC	b4175	0.59	-1.7
4,401,920	-	hflC	b4175	0.59	-0.9
4,401,920	-	hflC	b4175	0.59	-3.0
4,401,927	+	hflC	b4175	0.60	-2.3
4,401,958	+	hflC	b4175	0.63	-1.4
4,401,986	-	hflC	b4175	0.66	-2.4
4,401,986	-	hflC	b4175	0.66	-2.4
4,401,997	-	hflC	b4175	0.67	-2.6
4,401,997	-	hflC	b4175	0.67	-1.3
4,402,038	+	hflC	b4175	0.71	-1.7
4,402,038	+	hflC	b4175	0.71	-1.3
4,402,126	-	hflC	b4175	0.80	-1.7
4,402,126	-	hflC	b4175	0.80	-1.5
4,402,138	-	hflC	b4175	0.81	-3.6
4,402,166	-	hflC	b4175	0.84	-1.3
4,402,191	-	hflC	b4175	0.86	-2.8
4,402,220	-	hflC	b4175	0.89	-2.3
4,402,220	-	hflC	b4175	0.89	-0.5
4,402,253	-				-2.5
4,402,253	-				-2.5
4,402,258	+				-3.6
4,402,263	-				-3.3
4,402,263	-				-2.6
4,402,263	-				-0.5
4,402,263	-				-1.3
4,402,326	+				-1.5
4,402,336	+				+0.0
4,402,336	+				+0.8
4,402,341	+				-0.4
4,402,373	+				-0.1
4,402,441	+	yjeT	b4176	0.16	+0.8
4,402,449	-	yjeT	b4176	0.20	+0.3
4,402,484	-	yjeT	b4176	0.38	+1.5
4,402,497	-	yjeT	b4176	0.44	-0.3
4,402,930	-	purA	b4177	0.17	+1.1
4,402,990	-	purA	b4177	0.22	+1.1
4,403,242	-	purA	b4177	0.41	-0.5