Strain Fitness in Escherichia coli BW25113 around b3781

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrhlB and trxA are separated by 130 nucleotidestrxA and rho are separated by 326 nucleotides b3780: rhlB - ATP-dependent RNA helicase (NCBI), at 3,962,388 to 3,963,653 rhlB b3781: trxA - thioredoxin 1 (VIMSS), at 3,963,784 to 3,964,113 trxA b3783: rho - transcription termination factor Rho (NCBI), at 3,964,440 to 3,965,699 rho Position (kb) 3963 3964 3965Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3962.803 kb on - strand, within rhlBat 3962.811 kb on - strand, within rhlBat 3962.822 kb on + strand, within rhlBat 3962.840 kb on + strand, within rhlBat 3962.863 kb on - strand, within rhlBat 3962.863 kb on - strand, within rhlBat 3962.889 kb on - strand, within rhlBat 3962.942 kb on + strand, within rhlBat 3962.957 kb on + strand, within rhlBat 3962.957 kb on + strand, within rhlBat 3963.061 kb on + strand, within rhlBat 3963.110 kb on - strand, within rhlBat 3963.117 kb on - strand, within rhlBat 3963.120 kb on + strand, within rhlBat 3963.139 kb on + strand, within rhlBat 3963.139 kb on + strand, within rhlBat 3963.149 kb on + strand, within rhlBat 3963.196 kb on - strand, within rhlBat 3963.196 kb on - strand, within rhlBat 3963.207 kb on + strand, within rhlBat 3963.265 kb on + strand, within rhlBat 3963.265 kb on + strand, within rhlBat 3963.268 kb on - strand, within rhlBat 3963.275 kb on + strand, within rhlBat 3963.288 kb on - strand, within rhlBat 3963.295 kb on + strand, within rhlBat 3963.295 kb on + strand, within rhlBat 3963.346 kb on - strand, within rhlBat 3963.417 kb on - strand, within rhlBat 3963.417 kb on - strand, within rhlBat 3963.445 kb on - strand, within rhlBat 3963.450 kb on - strand, within rhlBat 3963.503 kb on - strand, within rhlBat 3963.513 kb on - strand, within rhlBat 3963.542 kb on + strandat 3963.542 kb on + strandat 3963.550 kb on - strandat 3963.553 kb on + strandat 3963.553 kb on + strandat 3963.561 kb on - strandat 3963.561 kb on - strandat 3963.605 kb on - strandat 3963.632 kb on - strandat 3963.655 kb on + strandat 3963.655 kb on + strandat 3963.694 kb on - strandat 3963.758 kb on - strandat 3963.764 kb on - strandat 3963.769 kb on - strandat 3963.780 kb on + strandat 3963.788 kb on - strandat 3963.788 kb on - strandat 3963.829 kb on + strandat 3963.837 kb on - strand, within trxAat 3963.839 kb on + strand, within trxAat 3963.856 kb on - strand, within trxAat 3963.957 kb on + strand, within trxAat 3963.975 kb on + strand, within trxAat 3963.975 kb on + strand, within trxAat 3963.975 kb on + strand, within trxAat 3964.021 kb on - strand, within trxAat 3964.026 kb on - strand, within trxAat 3964.068 kb on + strand, within trxAat 3964.068 kb on + strandat 3964.080 kb on + strand, within trxAat 3964.110 kb on - strandat 3964.110 kb on - strandat 3964.155 kb on + strandat 3964.179 kb on + strandat 3964.254 kb on + strandat 3964.308 kb on + strandat 3964.318 kb on - strandat 3964.320 kb on + strandat 3964.320 kb on + strandat 3964.320 kb on + strandat 3964.355 kb on + strandat 3964.378 kb on + strandat 3964.386 kb on - strandat 3964.460 kb on + strandat 3964.481 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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3,962,803 - rhlB b3780 0.33 -0.4
3,962,811 - rhlB b3780 0.33 -0.6
3,962,822 + rhlB b3780 0.34 +0.4
3,962,840 + rhlB b3780 0.36 -0.3
3,962,863 - rhlB b3780 0.38 +1.1
3,962,863 - rhlB b3780 0.38 +0.5
3,962,889 - rhlB b3780 0.40 +0.2
3,962,942 + rhlB b3780 0.44 +0.0
3,962,957 + rhlB b3780 0.45 -0.7
3,962,957 + rhlB b3780 0.45 +1.2
3,963,061 + rhlB b3780 0.53 -0.9
3,963,110 - rhlB b3780 0.57 +1.2
3,963,117 - rhlB b3780 0.58 -0.9
3,963,120 + rhlB b3780 0.58 +1.4
3,963,139 + rhlB b3780 0.59 -0.2
3,963,139 + rhlB b3780 0.59 +0.4
3,963,149 + rhlB b3780 0.60 +0.5
3,963,196 - rhlB b3780 0.64 -0.2
3,963,196 - rhlB b3780 0.64 +0.8
3,963,207 + rhlB b3780 0.65 +0.9
3,963,265 + rhlB b3780 0.69 -1.2
3,963,265 + rhlB b3780 0.69 +1.0
3,963,268 - rhlB b3780 0.70 +0.3
3,963,275 + rhlB b3780 0.70 +0.3
3,963,288 - rhlB b3780 0.71 -0.2
3,963,295 + rhlB b3780 0.72 +0.2
3,963,295 + rhlB b3780 0.72 +0.1
3,963,346 - rhlB b3780 0.76 -0.5
3,963,417 - rhlB b3780 0.81 -1.4
3,963,417 - rhlB b3780 0.81 +1.2
3,963,445 - rhlB b3780 0.83 -0.4
3,963,450 - rhlB b3780 0.84 +0.3
3,963,503 - rhlB b3780 0.88 +0.2
3,963,513 - rhlB b3780 0.89 -1.2
3,963,542 + +0.3
3,963,542 + +0.1
3,963,550 - +0.3
3,963,553 + +1.1
3,963,553 + -0.5
3,963,561 - +0.1
3,963,561 - -0.2
3,963,605 - +0.3
3,963,632 - +0.4
3,963,655 + +0.2
3,963,655 + +0.5
3,963,694 - +0.3
3,963,758 - -1.4
3,963,764 - -1.8
3,963,769 - -0.4
3,963,780 + +2.2
3,963,788 - +0.3
3,963,788 - -0.2
3,963,829 + -0.8
3,963,837 - trxA b3781 0.16 -2.0
3,963,839 + trxA b3781 0.17 -1.2
3,963,856 - trxA b3781 0.22 -0.4
3,963,957 + trxA b3781 0.52 -0.4
3,963,975 + trxA b3781 0.58 +2.8
3,963,975 + trxA b3781 0.58 -3.5
3,963,975 + trxA b3781 0.58 -2.2
3,964,021 - trxA b3781 0.72 -1.2
3,964,026 - trxA b3781 0.73 -0.1
3,964,068 + trxA b3781 0.86 -0.5
3,964,068 + +1.2
3,964,080 + trxA b3781 0.90 -3.2
3,964,110 - -0.4
3,964,110 - +0.2
3,964,155 + -1.2
3,964,179 + +0.9
3,964,254 + +0.5
3,964,308 + +0.0
3,964,318 - +0.6
3,964,320 + +0.9
3,964,320 + +0.3
3,964,320 + -0.1
3,964,355 + -0.8
3,964,378 + -0.4
3,964,386 - -0.8
3,964,460 + +0.5
3,964,481 + +2.2

Or see this region's nucleotide sequence