Strain Fitness in Escherichia coli BW25113 around b3726

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphoU and pstB are separated by 14 nucleotidespstB and pstA are separated by 182 nucleotidespstA and pstC overlap by 1 nucleotides b3724: phoU - negative regulator of PhoR/PhoB two-component regulator (NCBI), at 3,904,876 to 3,905,601 phoU b3725: pstB - phosphate transporter subunit (NCBI), at 3,905,616 to 3,906,389 pstB b3726: pstA - phosphate transporter subunit (NCBI), at 3,906,572 to 3,907,462 pstA b3727: pstC - phosphate transporter subunit (NCBI), at 3,907,462 to 3,908,421 pstC Position (kb) 3906 3907 3908Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3905.604 kb on - strandat 3905.604 kb on - strandat 3905.604 kb on - strandat 3905.608 kb on + strandat 3905.665 kb on - strandat 3905.700 kb on + strand, within pstBat 3906.128 kb on + strand, within pstBat 3906.136 kb on - strand, within pstBat 3906.381 kb on - strandat 3906.387 kb on - strandat 3906.468 kb on - strandat 3906.483 kb on + strandat 3906.493 kb on - strandat 3906.493 kb on - strandat 3906.524 kb on - strandat 3906.526 kb on + strandat 3906.538 kb on + strandat 3906.538 kb on + strandat 3906.538 kb on + strandat 3906.538 kb on + strandat 3906.546 kb on - strandat 3906.546 kb on - strandat 3906.546 kb on - strandat 3906.546 kb on - strandat 3906.546 kb on - strandat 3906.574 kb on - strandat 3906.574 kb on - strandat 3906.579 kb on + strandat 3906.587 kb on - strandat 3906.587 kb on - strandat 3906.614 kb on + strandat 3906.642 kb on - strandat 3906.642 kb on - strandat 3906.644 kb on - strandat 3906.779 kb on - strand, within pstAat 3906.835 kb on - strand, within pstAat 3906.836 kb on - strand, within pstAat 3906.888 kb on - strand, within pstAat 3906.905 kb on - strand, within pstAat 3906.918 kb on + strand, within pstAat 3906.928 kb on - strand, within pstAat 3906.951 kb on - strand, within pstAat 3906.992 kb on - strand, within pstAat 3907.077 kb on - strand, within pstAat 3907.084 kb on - strand, within pstAat 3907.084 kb on - strand, within pstAat 3907.089 kb on - strand, within pstAat 3907.199 kb on - strand, within pstAat 3907.218 kb on - strand, within pstAat 3907.218 kb on - strand, within pstAat 3907.236 kb on - strand, within pstAat 3907.236 kb on - strand, within pstAat 3907.337 kb on - strand, within pstAat 3907.459 kb on - strandat 3907.474 kb on + strandat 3907.477 kb on + strandat 3907.489 kb on + strandat 3907.561 kb on - strand, within pstCat 3907.580 kb on - strand, within pstCat 3907.619 kb on - strand, within pstCat 3907.619 kb on - strand, within pstCat 3907.634 kb on + strand, within pstCat 3907.634 kb on + strand, within pstCat 3907.634 kb on + strand, within pstCat 3907.645 kb on - strand, within pstCat 3907.647 kb on - strand, within pstCat 3907.675 kb on - strand, within pstCat 3907.675 kb on - strand, within pstCat 3907.727 kb on - strand, within pstCat 3907.769 kb on - strand, within pstCat 3907.818 kb on - strand, within pstCat 3908.058 kb on - strand, within pstCat 3908.304 kb on - strand, within pstCat 3908.435 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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3,905,604 - -2.5
3,905,604 - +0.5
3,905,604 - -0.9
3,905,608 + -1.5
3,905,665 - +1.2
3,905,700 + pstB b3725 0.11 +1.2
3,906,128 + pstB b3725 0.66 +1.2
3,906,136 - pstB b3725 0.67 +0.2
3,906,381 - +0.2
3,906,387 - -3.0
3,906,468 - +0.6
3,906,483 + -2.1
3,906,493 - -1.5
3,906,493 - +1.8
3,906,524 - +0.1
3,906,526 + -1.4
3,906,538 + -0.4
3,906,538 + +0.2
3,906,538 + -2.3
3,906,538 + -2.1
3,906,546 - -1.1
3,906,546 - -0.4
3,906,546 - +0.1
3,906,546 - -0.4
3,906,546 - -0.7
3,906,574 - -0.0
3,906,574 - -0.7
3,906,579 + -0.8
3,906,587 - +0.0
3,906,587 - +1.9
3,906,614 + +0.2
3,906,642 - -0.4
3,906,642 - +1.2
3,906,644 - +0.0
3,906,779 - pstA b3726 0.23 -1.1
3,906,835 - pstA b3726 0.30 +1.5
3,906,836 - pstA b3726 0.30 -0.4
3,906,888 - pstA b3726 0.35 +0.6
3,906,905 - pstA b3726 0.37 -1.9
3,906,918 + pstA b3726 0.39 +1.2
3,906,928 - pstA b3726 0.40 -1.4
3,906,951 - pstA b3726 0.43 -0.8
3,906,992 - pstA b3726 0.47 -0.4
3,907,077 - pstA b3726 0.57 +0.2
3,907,084 - pstA b3726 0.57 -1.6
3,907,084 - pstA b3726 0.57 -0.8
3,907,089 - pstA b3726 0.58 -1.1
3,907,199 - pstA b3726 0.70 -2.1
3,907,218 - pstA b3726 0.73 -2.0
3,907,218 - pstA b3726 0.73 +0.8
3,907,236 - pstA b3726 0.75 +0.5
3,907,236 - pstA b3726 0.75 +1.2
3,907,337 - pstA b3726 0.86 -1.8
3,907,459 - +0.0
3,907,474 + +2.2
3,907,477 + +0.2
3,907,489 + -1.0
3,907,561 - pstC b3727 0.10 -1.4
3,907,580 - pstC b3727 0.12 +1.9
3,907,619 - pstC b3727 0.16 +0.4
3,907,619 - pstC b3727 0.16 -0.6
3,907,634 + pstC b3727 0.18 +1.2
3,907,634 + pstC b3727 0.18 +1.2
3,907,634 + pstC b3727 0.18 +1.2
3,907,645 - pstC b3727 0.19 -2.1
3,907,647 - pstC b3727 0.19 -3.0
3,907,675 - pstC b3727 0.22 +0.3
3,907,675 - pstC b3727 0.22 -1.1
3,907,727 - pstC b3727 0.28 -0.8
3,907,769 - pstC b3727 0.32 -0.4
3,907,818 - pstC b3727 0.37 +1.2
3,908,058 - pstC b3727 0.62 -0.4
3,908,304 - pstC b3727 0.88 -0.4
3,908,435 + +1.2

Or see this region's nucleotide sequence