Strain Fitness in Escherichia coli BW25113 around b3531

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Aerobic with Hygromycin B 0.000015625 mM
3,686,207	-	bcsC	b3530	0.72	+0.2
3,686,240	-	bcsC	b3530	0.72	+0.1
3,686,267	+	bcsC	b3530	0.73	+2.2
3,686,267	+	bcsC	b3530	0.73	+0.7
3,686,311	-	bcsC	b3530	0.74	+0.1
3,686,311	-	bcsC	b3530	0.74	+0.1
3,686,311	-	bcsC	b3530	0.74	-0.1
3,686,313	+	bcsC	b3530	0.75	-0.4
3,686,313	+	bcsC	b3530	0.75	+0.5
3,686,315	+	bcsC	b3530	0.75	+0.2
3,686,321	-	bcsC	b3530	0.75	-0.1
3,686,321	-	bcsC	b3530	0.75	-0.5
3,686,334	+	bcsC	b3530	0.75	-1.2
3,686,389	-	bcsC	b3530	0.77	+0.6
3,686,493	+	bcsC	b3530	0.80	+1.4
3,686,504	+	bcsC	b3530	0.80	-0.1
3,686,506	-	bcsC	b3530	0.80	+0.4
3,686,522	+	bcsC	b3530	0.81	+0.6
3,686,525	-	bcsC	b3530	0.81	-1.5
3,686,579	+	bcsC	b3530	0.82	-0.2
3,686,605	-	bcsC	b3530	0.83	-0.3
3,686,605	-	bcsC	b3530	0.83	+1.2
3,686,621	+	bcsC	b3530	0.83	-1.1
3,686,624	-	bcsC	b3530	0.84	+0.6
3,686,624	-	bcsC	b3530	0.84	+0.4
3,686,783	-	bcsC	b3530	0.88	-0.7
3,686,783	-	bcsC	b3530	0.88	-0.7
3,686,783	-	bcsC	b3530	0.88	-0.5
3,686,831	+	bcsC	b3530	0.89	-0.3
3,686,851	+				+0.6
3,686,851	-				+0.6
3,686,928	-				+0.4
3,686,928	-				-0.2
3,686,929	-				+1.2
3,686,929	-				-1.3
3,686,983	-				+0.9
3,687,083	+				+0.4
3,687,083	+				-0.7
3,687,098	+				+1.2
3,687,098	+				+0.8
3,687,127	-				-0.3
3,687,127	-				-0.0
3,687,127	-				+0.4
3,687,175	+				-1.4
3,687,204	-				+0.0
3,687,247	+				-0.2
3,687,254	+				-0.3
3,687,254	+				+0.0
3,687,254	+				-0.3
3,687,254	+				+1.2
3,687,271	-				+0.7
3,687,271	-				+0.4
3,687,272	-				-0.4
3,687,286	+				+0.5
3,687,324	+	bcsZ	b3531	0.13	+1.1
3,687,334	-	bcsZ	b3531	0.14	-0.1
3,687,340	+	bcsZ	b3531	0.15	+0.2
3,687,404	-	bcsZ	b3531	0.20	+0.0
3,687,484	-	bcsZ	b3531	0.28	+0.3
3,687,509	-	bcsZ	b3531	0.30	+0.5
3,687,509	-	bcsZ	b3531	0.30	-0.4
3,687,523	-	bcsZ	b3531	0.31	+0.9
3,687,523	-	bcsZ	b3531	0.31	+0.0
3,687,546	+	bcsZ	b3531	0.33	+0.6
3,687,555	+	bcsZ	b3531	0.34	+0.5
3,687,555	+	bcsZ	b3531	0.34	-0.6
3,687,592	+	bcsZ	b3531	0.37	+0.1
3,687,658	-	bcsZ	b3531	0.43	-0.7
3,687,658	-	bcsZ	b3531	0.43	-0.2
3,687,689	+	bcsZ	b3531	0.46	+0.3
3,687,769	-	bcsZ	b3531	0.53	-0.0
3,687,792	+	bcsZ	b3531	0.55	+1.2
3,687,856	+	bcsZ	b3531	0.61	-0.4
3,687,916	-	bcsZ	b3531	0.67	+0.6
3,687,920	+	bcsZ	b3531	0.67	+0.2
3,687,997	-	bcsZ	b3531	0.74	-1.6
3,688,039	+	bcsZ	b3531	0.78	+0.6
3,688,102	-	bcsZ	b3531	0.83	-0.1
3,688,102	-	bcsZ	b3531	0.83	+0.8
3,688,168	+	bcsZ	b3531	0.89	+1.4
3,688,177	+				-0.3
3,688,183	-				+0.5
3,688,198	+				-0.5
3,688,213	+				+0.1
3,688,216	-				+0.3
3,688,216	-				+0.6
3,688,217	-				+0.8
3,688,221	-				-0.0
3,688,304	-				+0.4
3,688,306	-				-0.9
3,688,352	-				+0.5
3,688,352	-				+0.0
3,688,362	+				-0.5
3,688,362	+				-0.7
3,688,363	-				-0.4
3,688,375	-				+0.1
3,688,441	-				-0.3
3,688,571	+	bcsB	b3532	0.12	-0.7
3,688,571	-	bcsB	b3532	0.12	+0.3
3,688,582	+	bcsB	b3532	0.12	+0.2
3,688,583	+	bcsB	b3532	0.12	-0.6
3,688,604	-	bcsB	b3532	0.13	+0.8
3,688,604	-	bcsB	b3532	0.13	-1.8
3,688,634	+	bcsB	b3532	0.15	-0.9
3,688,634	+	bcsB	b3532	0.15	+0.2
3,688,671	-	bcsB	b3532	0.16	-0.6
3,688,702	+	bcsB	b3532	0.18	-0.5
3,688,707	+	bcsB	b3532	0.18	-0.2
3,688,727	+	bcsB	b3532	0.19	+1.0
3,688,727	+	bcsB	b3532	0.19	-0.4
3,688,749	-	bcsB	b3532	0.20	-1.6
3,688,770	-	bcsB	b3532	0.20	+0.3
3,688,778	-	bcsB	b3532	0.21	+2.2
3,688,778	-	bcsB	b3532	0.21	-1.6
3,688,783	-	bcsB	b3532	0.21	-0.7
3,688,814	+	bcsB	b3532	0.22	+0.2
3,688,814	+	bcsB	b3532	0.22	-0.6
3,688,833	+	bcsB	b3532	0.23	+0.0
3,688,843	+	bcsB	b3532	0.24	+0.2
3,688,843	+	bcsB	b3532	0.24	-0.1
3,688,861	-	bcsB	b3532	0.24	+0.4
3,688,868	+	bcsB	b3532	0.25	+0.1
3,688,897	+	bcsB	b3532	0.26	-0.3
3,688,930	-	bcsB	b3532	0.27	+0.8
3,688,944	+	bcsB	b3532	0.28	+0.8
3,688,944	+	bcsB	b3532	0.28	+1.3
3,688,944	+	bcsB	b3532	0.28	-0.2
3,688,944	+	bcsB	b3532	0.28	+0.4
3,688,981	+	bcsB	b3532	0.29	+0.8
3,688,981	+	bcsB	b3532	0.29	+1.2
3,689,014	-	bcsB	b3532	0.31	+0.6
3,689,030	-	bcsB	b3532	0.32	-0.9
3,689,030	-	bcsB	b3532	0.32	+1.5
3,689,076	+	bcsB	b3532	0.34	-0.6
3,689,076	+	bcsB	b3532	0.34	+0.0
3,689,086	+	bcsB	b3532	0.34	+0.5
3,689,125	-	bcsB	b3532	0.36	+0.1
3,689,125	-	bcsB	b3532	0.36	-0.6
3,689,126	+	bcsB	b3532	0.36	-1.5
3,689,126	+	bcsB	b3532	0.36	-0.4
3,689,134	-	bcsB	b3532	0.36	-0.6
3,689,134	-	bcsB	b3532	0.36	+0.5
3,689,161	+	bcsB	b3532	0.37	-0.1
3,689,164	-	bcsB	b3532	0.37	-0.1
3,689,164	-	bcsB	b3532	0.37	+1.2
3,689,224	-	bcsB	b3532	0.40	-1.4