Strain Fitness in Escherichia coli BW25113 around b2895

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntxerD and fldB are separated by 111 nucleotidesfldB and ygfX are separated by 39 nucleotidesygfX and ygfY overlap by 20 nucleotidesygfY and ygfZ are separated by 242 nucleotides b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765 xerD b2895: fldB - flavodoxin 2 (NCBI), at 3,037,877 to 3,038,398 fldB b2896: ygfX - hypothetical protein (NCBI), at 3,038,438 to 3,038,845 ygfX b2897: ygfY - hypothetical protein (NCBI), at 3,038,826 to 3,039,092 ygfY b2898: ygfZ - putative global regulator (NCBI), at 3,039,335 to 3,040,315 ygfZ Position (kb) 3037 3038 3039Strain fitness (log2 ratio) -2 -1 0 1 2at 3037.372 kb on + strand, within xerDat 3037.773 kb on + strandat 3037.777 kb on + strandat 3037.777 kb on + strandat 3037.900 kb on + strandat 3037.900 kb on + strandat 3037.986 kb on - strand, within fldBat 3038.097 kb on + strand, within fldBat 3038.128 kb on + strand, within fldBat 3038.128 kb on + strand, within fldBat 3038.128 kb on + strand, within fldBat 3038.149 kb on + strand, within fldBat 3038.149 kb on + strand, within fldBat 3038.321 kb on + strand, within fldBat 3038.348 kb on - strandat 3038.348 kb on - strandat 3038.418 kb on - strandat 3038.516 kb on - strand, within ygfXat 3038.698 kb on - strand, within ygfXat 3038.714 kb on + strand, within ygfXat 3038.714 kb on + strand, within ygfXat 3038.716 kb on - strand, within ygfXat 3038.718 kb on + strand, within ygfXat 3038.718 kb on + strand, within ygfXat 3038.746 kb on - strand, within ygfXat 3038.776 kb on + strand, within ygfXat 3038.785 kb on - strand, within ygfXat 3038.785 kb on - strand, within ygfXat 3038.869 kb on + strand, within ygfYat 3038.877 kb on - strand, within ygfYat 3038.877 kb on - strand, within ygfYat 3038.992 kb on - strand, within ygfYat 3039.002 kb on - strand, within ygfYat 3039.028 kb on + strand, within ygfYat 3039.170 kb on - strandat 3039.170 kb on - strandat 3039.251 kb on - strandat 3039.251 kb on - strandat 3039.255 kb on - strandat 3039.298 kb on + strandat 3039.320 kb on + strandat 3039.320 kb on + strandat 3039.328 kb on - strandat 3039.379 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
remove
3,037,372 + xerD b2894 0.56 -1.1
3,037,773 + -0.1
3,037,777 + +0.9
3,037,777 + +0.1
3,037,900 + -0.1
3,037,900 + +0.0
3,037,986 - fldB b2895 0.21 -0.1
3,038,097 + fldB b2895 0.42 +2.4
3,038,128 + fldB b2895 0.48 -0.7
3,038,128 + fldB b2895 0.48 -1.3
3,038,128 + fldB b2895 0.48 -0.1
3,038,149 + fldB b2895 0.52 -2.7
3,038,149 + fldB b2895 0.52 -0.4
3,038,321 + fldB b2895 0.85 -0.7
3,038,348 - +0.7
3,038,348 - +1.3
3,038,418 - +0.5
3,038,516 - ygfX b2896 0.19 +0.2
3,038,698 - ygfX b2896 0.64 -0.3
3,038,714 + ygfX b2896 0.68 -1.3
3,038,714 + ygfX b2896 0.68 -0.4
3,038,716 - ygfX b2896 0.68 -0.9
3,038,718 + ygfX b2896 0.69 +0.3
3,038,718 + ygfX b2896 0.69 +1.1
3,038,746 - ygfX b2896 0.75 -0.0
3,038,776 + ygfX b2896 0.83 +0.4
3,038,785 - ygfX b2896 0.85 -0.3
3,038,785 - ygfX b2896 0.85 +0.2
3,038,869 + ygfY b2897 0.16 -0.1
3,038,877 - ygfY b2897 0.19 -0.5
3,038,877 - ygfY b2897 0.19 +1.9
3,038,992 - ygfY b2897 0.62 -0.5
3,039,002 - ygfY b2897 0.66 -1.4
3,039,028 + ygfY b2897 0.76 -0.6
3,039,170 - +0.4
3,039,170 - +0.3
3,039,251 - +0.3
3,039,251 - +0.6
3,039,255 - -0.1
3,039,298 + +0.0
3,039,320 + +0.7
3,039,320 + -0.1
3,039,328 - -0.5
3,039,379 - -1.5

Or see this region's nucleotide sequence