Strain Fitness in Escherichia coli BW25113 around b1594

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1592 and b1593 overlap by 48 nucleotidesb1593 and dgsA are separated by 124 nucleotidesdgsA and ynfL are separated by 134 nucleotides b1592: b1592 - putative chloride channel (VIMSS), at 1,663,339 to 1,664,595 b1592 b1593: b1593 - orf, hypothetical protein (VIMSS), at 1,664,548 to 1,665,243 b1593 b1594: dgsA - DNA-binding transcriptional repressor (NCBI), at 1,665,368 to 1,666,588 dgsA b1595: ynfL - predicted DNA-binding transcriptional regulator (NCBI), at 1,666,723 to 1,667,616 ynfL Position (kb) 1665 1666 1667Strain fitness (log2 ratio) -2 -1 0 1at 1664.448 kb on + strand, within b1592at 1664.448 kb on + strand, within b1592at 1664.579 kb on - strandat 1664.586 kb on + strandat 1664.586 kb on + strandat 1664.976 kb on + strand, within b1593at 1664.983 kb on + strand, within b1593at 1664.983 kb on + strand, within b1593at 1664.991 kb on - strand, within b1593at 1664.991 kb on - strand, within b1593at 1664.991 kb on - strand, within b1593at 1665.407 kb on - strandat 1665.479 kb on - strandat 1665.479 kb on - strandat 1665.479 kb on - strandat 1665.479 kb on - strandat 1665.493 kb on - strand, within dgsAat 1665.645 kb on - strand, within dgsAat 1665.645 kb on - strand, within dgsAat 1665.781 kb on - strand, within dgsAat 1665.781 kb on - strand, within dgsAat 1665.898 kb on + strand, within dgsAat 1665.919 kb on + strand, within dgsAat 1665.919 kb on + strand, within dgsAat 1666.141 kb on - strand, within dgsAat 1666.300 kb on + strand, within dgsAat 1666.300 kb on + strand, within dgsAat 1666.471 kb on - strandat 1666.515 kb on - strandat 1666.726 kb on + strandat 1666.726 kb on + strandat 1666.732 kb on - strandat 1666.732 kb on - strandat 1667.012 kb on - strand, within ynfLat 1667.263 kb on - strand, within ynfLat 1667.263 kb on - strand, within ynfLat 1667.440 kb on + strand, within ynfLat 1667.445 kb on + strand, within ynfLat 1667.445 kb on + strand, within ynfL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,664,448 + b1592 b1592 0.88 -0.3
1,664,448 + b1592 b1592 0.88 -0.3
1,664,579 - +0.0
1,664,586 + -0.7
1,664,586 + +1.3
1,664,976 + b1593 b1593 0.61 -0.2
1,664,983 + b1593 b1593 0.62 +0.3
1,664,983 + b1593 b1593 0.62 -2.0
1,664,991 - b1593 b1593 0.64 +1.5
1,664,991 - b1593 b1593 0.64 -0.7
1,664,991 - b1593 b1593 0.64 +0.2
1,665,407 - -0.2
1,665,479 - -0.8
1,665,479 - +0.6
1,665,479 - -1.7
1,665,479 - +0.3
1,665,493 - dgsA b1594 0.10 -0.4
1,665,645 - dgsA b1594 0.23 -0.7
1,665,645 - dgsA b1594 0.23 -0.7
1,665,781 - dgsA b1594 0.34 -1.7
1,665,781 - dgsA b1594 0.34 -2.0
1,665,898 + dgsA b1594 0.43 +0.6
1,665,919 + dgsA b1594 0.45 -1.7
1,665,919 + dgsA b1594 0.45 +0.3
1,666,141 - dgsA b1594 0.63 -0.5
1,666,300 + dgsA b1594 0.76 -0.3
1,666,300 + dgsA b1594 0.76 +1.3
1,666,471 - -0.4
1,666,515 - -0.8
1,666,726 + -0.5
1,666,726 + -1.9
1,666,732 - +1.1
1,666,732 - +0.7
1,667,012 - ynfL b1595 0.32 +0.4
1,667,263 - ynfL b1595 0.60 -0.0
1,667,263 - ynfL b1595 0.60 -0.3
1,667,440 + ynfL b1595 0.80 +0.3
1,667,445 + ynfL b1595 0.81 -0.7
1,667,445 + ynfL b1595 0.81 +0.3

Or see this region's nucleotide sequence