Strain Fitness in Escherichia coli BW25113 around b1502

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntYdeP and ydeQ are separated by 333 nucleotidesydeQ and ydeR are separated by 58 nucleotidesydeR and ydeS are separated by 12 nucleotides b1501: YdeP - putative oxidoreductase, major subunit, acid resistance protein (Katherine Huang), at 1,582,231 to 1,584,510 YdeP b1502: ydeQ - predicted fimbrial-like adhesin protein (NCBI), at 1,584,844 to 1,585,758 ydeQ b1503: ydeR - predicted fimbrial-like adhesin protein (NCBI), at 1,585,817 to 1,586,320 ydeR b1504: ydeS - predicted fimbrial-like adhesin protein (NCBI), at 1,586,333 to 1,586,863 ydeS Position (kb) 1584 1585 1586Strain fitness (log2 ratio) -2 -1 0 1at 1583.951 kb on + strand, within YdePat 1583.980 kb on - strand, within YdePat 1583.980 kb on - strand, within YdePat 1584.084 kb on + strand, within YdePat 1584.133 kb on + strand, within YdePat 1584.178 kb on - strand, within YdePat 1584.178 kb on - strand, within YdePat 1584.205 kb on + strand, within YdePat 1584.334 kb on + strandat 1584.337 kb on - strandat 1584.367 kb on + strandat 1584.367 kb on + strandat 1584.375 kb on + strandat 1584.375 kb on + strandat 1584.419 kb on + strandat 1584.481 kb on + strandat 1584.556 kb on + strandat 1584.556 kb on + strandat 1584.564 kb on + strandat 1584.566 kb on + strandat 1584.566 kb on + strandat 1584.566 kb on + strandat 1584.838 kb on - strandat 1584.993 kb on - strand, within ydeQat 1585.024 kb on - strand, within ydeQat 1585.026 kb on - strand, within ydeQat 1585.054 kb on - strand, within ydeQat 1585.490 kb on - strand, within ydeQat 1585.490 kb on - strand, within ydeQat 1585.490 kb on - strand, within ydeQat 1585.490 kb on - strand, within ydeQat 1585.872 kb on - strand, within ydeRat 1585.981 kb on - strand, within ydeRat 1586.062 kb on - strand, within ydeRat 1586.062 kb on - strand, within ydeRat 1586.105 kb on - strand, within ydeRat 1586.172 kb on + strand, within ydeRat 1586.187 kb on + strand, within ydeRat 1586.200 kb on - strand, within ydeRat 1586.200 kb on - strand, within ydeRat 1586.210 kb on - strand, within ydeRat 1586.217 kb on - strand, within ydeRat 1586.239 kb on - strand, within ydeRat 1586.245 kb on - strand, within ydeRat 1586.252 kb on + strand, within ydeRat 1586.260 kb on - strand, within ydeRat 1586.364 kb on + strandat 1586.372 kb on - strandat 1586.408 kb on - strand, within ydeSat 1586.415 kb on + strand, within ydeSat 1586.415 kb on + strand, within ydeSat 1586.415 kb on + strand, within ydeSat 1586.415 kb on + strand, within ydeSat 1586.415 kb on + strand, within ydeSat 1586.458 kb on + strand, within ydeSat 1586.589 kb on + strand, within ydeSat 1586.659 kb on + strand, within ydeSat 1586.748 kb on + strand, within ydeS

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,583,951 + YdeP b1501 0.75 -0.9
1,583,980 - YdeP b1501 0.77 +0.1
1,583,980 - YdeP b1501 0.77 +0.1
1,584,084 + YdeP b1501 0.81 +0.9
1,584,133 + YdeP b1501 0.83 -1.7
1,584,178 - YdeP b1501 0.85 +0.5
1,584,178 - YdeP b1501 0.85 +1.0
1,584,205 + YdeP b1501 0.87 -0.3
1,584,334 + -1.7
1,584,337 - +0.1
1,584,367 + +0.3
1,584,367 + -2.0
1,584,375 + -1.7
1,584,375 + -0.7
1,584,419 + -0.7
1,584,481 + -0.5
1,584,556 + +1.5
1,584,556 + +0.3
1,584,564 + +0.3
1,584,566 + -0.1
1,584,566 + -0.1
1,584,566 + +0.9
1,584,838 - -0.7
1,584,993 - ydeQ b1502 0.16 +1.3
1,585,024 - ydeQ b1502 0.20 +0.3
1,585,026 - ydeQ b1502 0.20 +0.3
1,585,054 - ydeQ b1502 0.23 -2.0
1,585,490 - ydeQ b1502 0.71 -1.3
1,585,490 - ydeQ b1502 0.71 +0.3
1,585,490 - ydeQ b1502 0.71 -1.3
1,585,490 - ydeQ b1502 0.71 +0.3
1,585,872 - ydeR b1503 0.11 -0.7
1,585,981 - ydeR b1503 0.33 +1.3
1,586,062 - ydeR b1503 0.49 -1.3
1,586,062 - ydeR b1503 0.49 +0.3
1,586,105 - ydeR b1503 0.57 -0.7
1,586,172 + ydeR b1503 0.70 +0.3
1,586,187 + ydeR b1503 0.73 +0.3
1,586,200 - ydeR b1503 0.76 -0.3
1,586,200 - ydeR b1503 0.76 -0.7
1,586,210 - ydeR b1503 0.78 -0.1
1,586,217 - ydeR b1503 0.79 +0.3
1,586,239 - ydeR b1503 0.84 -1.3
1,586,245 - ydeR b1503 0.85 -2.0
1,586,252 + ydeR b1503 0.86 +1.3
1,586,260 - ydeR b1503 0.88 -0.3
1,586,364 + +0.1
1,586,372 - +0.9
1,586,408 - ydeS b1504 0.14 +0.3
1,586,415 + ydeS b1504 0.15 +0.3
1,586,415 + ydeS b1504 0.15 +1.3
1,586,415 + ydeS b1504 0.15 +0.2
1,586,415 + ydeS b1504 0.15 -1.1
1,586,415 + ydeS b1504 0.15 -1.3
1,586,458 + ydeS b1504 0.24 +1.7
1,586,589 + ydeS b1504 0.48 +0.3
1,586,659 + ydeS b1504 0.61 +0.3
1,586,748 + ydeS b1504 0.78 -0.7

Or see this region's nucleotide sequence