Strain Fitness in Escherichia coli BW25113 around b1418

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgapC and cybB are separated by 188 nucleotidescybB and rydC are separated by 10 nucleotidesrydC and ydcA are separated by 170 nucleotidesydcA and hokB are separated by 71 nucleotideshokB and mokB overlap by 110 nucleotidesmokB and sokB overlap by 11 nucleotides b4493: gapC - no description, at 1,487,737 to 1,488,737 gapC b1418: cybB - cytochrome b(561) (VIMSS), at 1,488,926 to 1,489,456 cybB b4597: rydC - sRNA regulator of yejABEF (RefSeq), at 1,489,467 to 1,489,530 rydC b1419: ydcA - hypothetical protein (NCBI), at 1,489,701 to 1,489,874 ydcA b4428: hokB - toxic polypeptide, small (NCBI), at 1,489,946 to 1,490,095 hokB b1420: mokB - regulatory peptide (NCBI), at 1,489,986 to 1,490,153 mokB b4429: sokB - Antisense sRNA blocking mokB, and hence hokB, translation (RefSeq), at 1,490,143 to 1,490,198 sokB Position (kb) 1488 1489 1490Strain fitness (log2 ratio) -1 0 1at 1488.063 kb on - strand, within gapCat 1488.092 kb on - strand, within gapCat 1488.654 kb on - strandat 1488.684 kb on + strandat 1488.684 kb on + strandat 1488.827 kb on + strandat 1489.026 kb on + strand, within cybBat 1489.028 kb on + strand, within cybBat 1489.032 kb on + strand, within cybBat 1489.032 kb on + strand, within cybBat 1489.616 kb on + strandat 1489.627 kb on + strandat 1489.651 kb on + strandat 1489.659 kb on - strandat 1489.659 kb on - strandat 1489.794 kb on + strand, within ydcAat 1489.794 kb on + strand, within ydcAat 1489.802 kb on - strand, within ydcAat 1489.802 kb on - strand, within ydcAat 1489.804 kb on + strand, within ydcAat 1489.804 kb on + strand, within ydcAat 1490.029 kb on + strandat 1490.063 kb on + strandat 1490.099 kb on - strand, within mokBat 1490.344 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,488,063 - gapC b4493 0.33 -0.1
1,488,092 - gapC b4493 0.35 +0.2
1,488,654 - +0.1
1,488,684 + +0.0
1,488,684 + -0.4
1,488,827 + -0.1
1,489,026 + cybB b1418 0.19 +0.9
1,489,028 + cybB b1418 0.19 -1.6
1,489,032 + cybB b1418 0.20 +1.3
1,489,032 + cybB b1418 0.20 -0.7
1,489,616 + -0.2
1,489,627 + -0.3
1,489,651 + -0.9
1,489,659 - -0.4
1,489,659 - +1.3
1,489,794 + ydcA b1419 0.53 +0.3
1,489,794 + ydcA b1419 0.53 -0.1
1,489,802 - ydcA b1419 0.58 +0.3
1,489,802 - ydcA b1419 0.58 +0.2
1,489,804 + ydcA b1419 0.59 +0.7
1,489,804 + ydcA b1419 0.59 +0.2
1,490,029 + -0.5
1,490,063 + +0.4
1,490,099 - mokB b1420 0.67 +0.3
1,490,344 - -0.4

Or see this region's nucleotide sequence