Experiment: LB Aerobic with Hygromycin B 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gapC and cybB are separated by 188 nucleotides cybB and rydC are separated by 10 nucleotides rydC and ydcA are separated by 170 nucleotides ydcA and hokB are separated by 71 nucleotides hokB and mokB overlap by 110 nucleotides mokB and sokB overlap by 11 nucleotides
b4493: gapC - no description, at 1,487,737 to 1,488,737
gapC
b1418: cybB - cytochrome b(561) (VIMSS), at 1,488,926 to 1,489,456
cybB
b4597: rydC - sRNA regulator of yejABEF (RefSeq), at 1,489,467 to 1,489,530
rydC
b1419: ydcA - hypothetical protein (NCBI), at 1,489,701 to 1,489,874
ydcA
b4428: hokB - toxic polypeptide, small (NCBI), at 1,489,946 to 1,490,095
hokB
b1420: mokB - regulatory peptide (NCBI), at 1,489,986 to 1,490,153
mokB
b4429: sokB - Antisense sRNA blocking mokB, and hence hokB, translation (RefSeq), at 1,490,143 to 1,490,198
sokB
Position (kb)
1488
1489
1490 Strain fitness (log2 ratio)
-1
0
1 at 1488.063 kb on - strand, within gapC at 1488.092 kb on - strand, within gapC at 1488.654 kb on - strand at 1488.684 kb on + strand at 1488.684 kb on + strand at 1488.827 kb on + strand at 1489.026 kb on + strand, within cybB at 1489.028 kb on + strand, within cybB at 1489.032 kb on + strand, within cybB at 1489.032 kb on + strand, within cybB at 1489.616 kb on + strand at 1489.627 kb on + strand at 1489.651 kb on + strand at 1489.659 kb on - strand at 1489.659 kb on - strand at 1489.794 kb on + strand, within ydcA at 1489.794 kb on + strand, within ydcA at 1489.802 kb on - strand, within ydcA at 1489.802 kb on - strand, within ydcA at 1489.804 kb on + strand, within ydcA at 1489.804 kb on + strand, within ydcA at 1490.029 kb on + strand at 1490.063 kb on + strand at 1490.099 kb on - strand, within mokB at 1490.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM remove 1,488,063 - gapC b4493 0.33 -0.1 1,488,092 - gapC b4493 0.35 +0.2 1,488,654 - +0.1 1,488,684 + +0.0 1,488,684 + -0.4 1,488,827 + -0.1 1,489,026 + cybB b1418 0.19 +0.9 1,489,028 + cybB b1418 0.19 -1.6 1,489,032 + cybB b1418 0.20 +1.3 1,489,032 + cybB b1418 0.20 -0.7 1,489,616 + -0.2 1,489,627 + -0.3 1,489,651 + -0.9 1,489,659 - -0.4 1,489,659 - +1.3 1,489,794 + ydcA b1419 0.53 +0.3 1,489,794 + ydcA b1419 0.53 -0.1 1,489,802 - ydcA b1419 0.58 +0.3 1,489,802 - ydcA b1419 0.58 +0.2 1,489,804 + ydcA b1419 0.59 +0.7 1,489,804 + ydcA b1419 0.59 +0.2 1,490,029 + -0.5 1,490,063 + +0.4 1,490,099 - mokB b1420 0.67 +0.3 1,490,344 - -0.4
Or see this region's nucleotide sequence