Strain Fitness in Escherichia coli BW25113 around b1380

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydbJ and hslJ overlap by 4 nucleotideshslJ and ldhA are separated by 110 nucleotidesldhA and ydbH are separated by 207 nucleotides b4529: ydbJ - hypothetical protein (NCBI), at 1,439,082 to 1,439,348 ydbJ b1379: hslJ - heat-inducible protein (NCBI), at 1,439,345 to 1,439,767 hslJ b1380: ldhA - D-lactate dehydrogenase (NCBI), at 1,439,878 to 1,440,867 ldhA b1381: ydbH - hypothetical protein (NCBI), at 1,441,075 to 1,443,714 ydbH Position (kb) 1439 1440 1441Strain fitness (log2 ratio) -2 -1 0 1at 1438.926 kb on - strandat 1438.926 kb on - strandat 1438.997 kb on + strandat 1438.998 kb on - strandat 1439.084 kb on - strandat 1439.084 kb on - strandat 1439.137 kb on - strand, within ydbJat 1439.365 kb on + strandat 1439.401 kb on - strand, within hslJat 1439.561 kb on - strand, within hslJat 1439.561 kb on - strand, within hslJat 1439.591 kb on + strand, within hslJat 1439.591 kb on + strand, within hslJat 1439.654 kb on + strand, within hslJat 1439.836 kb on - strandat 1439.836 kb on - strandat 1439.865 kb on - strandat 1439.929 kb on + strandat 1439.995 kb on - strand, within ldhAat 1440.256 kb on + strand, within ldhAat 1440.401 kb on - strand, within ldhAat 1440.401 kb on - strand, within ldhAat 1440.872 kb on - strandat 1440.994 kb on + strandat 1441.169 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,438,926 - -1.0
1,438,926 - -0.3
1,438,997 + -0.1
1,438,998 - -0.1
1,439,084 - -0.8
1,439,084 - -0.6
1,439,137 - ydbJ b4529 0.21 -1.4
1,439,365 + -0.2
1,439,401 - hslJ b1379 0.13 +0.7
1,439,561 - hslJ b1379 0.51 -0.0
1,439,561 - hslJ b1379 0.51 -0.8
1,439,591 + hslJ b1379 0.58 -0.2
1,439,591 + hslJ b1379 0.58 -0.2
1,439,654 + hslJ b1379 0.73 +1.1
1,439,836 - -0.7
1,439,836 - +0.4
1,439,865 - +0.1
1,439,929 + -0.3
1,439,995 - ldhA b1380 0.12 -0.0
1,440,256 + ldhA b1380 0.38 -1.7
1,440,401 - ldhA b1380 0.53 +0.3
1,440,401 - ldhA b1380 0.53 -2.1
1,440,872 - +0.3
1,440,994 + +1.3
1,441,169 + -0.0

Or see this region's nucleotide sequence