Experiment: LB Aerobic with Hygromycin B 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt intR and ydaQ are separated by 1 nucleotides ydaQ and ydaC are separated by 78 nucleotides ydaC and lar overlap by 8 nucleotides lar and recT are separated by 56 nucleotides recT and recE overlap by 8 nucleotides
b1345: intR - Rac prophage; integrase (NCBI), at 1,410,024 to 1,411,259
intR
b1346: ydaQ - orf, hypothetical protein (VIMSS), at 1,411,261 to 1,411,476
ydaQ
b1347: ydaC - Rac prophage; predicted protein (NCBI), at 1,411,555 to 1,411,764
ydaC
b1348: lar - Rac prophage; restriction alleviation protein (NCBI), at 1,411,757 to 1,411,951
lar
b1349: recT - Rac prophage; recombination and repair protein (NCBI), at 1,412,008 to 1,412,817
recT
b1350: recE - Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI), at 1,412,810 to 1,415,410
recE
Position (kb)
1411
1412 Strain fitness (log2 ratio)
-2
-1
0
1 at 1410.761 kb on + strand, within intR at 1410.788 kb on + strand, within intR at 1410.843 kb on - strand, within intR at 1410.879 kb on + strand, within intR at 1411.223 kb on - strand at 1411.308 kb on - strand, within ydaQ at 1411.308 kb on - strand, within ydaQ at 1411.364 kb on + strand, within ydaQ at 1411.483 kb on + strand at 1411.483 kb on + strand at 1411.534 kb on + strand at 1411.637 kb on - strand, within ydaC at 1411.696 kb on - strand, within ydaC at 1411.844 kb on - strand, within lar at 1411.855 kb on - strand, within lar at 1411.865 kb on - strand, within lar at 1411.865 kb on - strand, within lar at 1411.867 kb on - strand, within lar at 1411.880 kb on - strand, within lar at 1411.880 kb on - strand, within lar at 1412.066 kb on - strand at 1412.069 kb on - strand at 1412.069 kb on - strand at 1412.080 kb on - strand at 1412.090 kb on - strand, within recT at 1412.103 kb on - strand, within recT at 1412.103 kb on - strand, within recT at 1412.113 kb on - strand, within recT at 1412.192 kb on - strand, within recT at 1412.213 kb on - strand, within recT at 1412.213 kb on - strand, within recT at 1412.235 kb on - strand, within recT at 1412.302 kb on - strand, within recT at 1412.302 kb on - strand, within recT at 1412.538 kb on - strand, within recT at 1412.896 kb on + strand at 1412.896 kb on + strand at 1412.896 kb on - strand at 1412.896 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM remove 1,410,761 + intR b1345 0.60 +0.3 1,410,788 + intR b1345 0.62 +0.3 1,410,843 - intR b1345 0.66 +0.3 1,410,879 + intR b1345 0.69 +0.3 1,411,223 - +0.3 1,411,308 - ydaQ b1346 0.22 -2.5 1,411,308 - ydaQ b1346 0.22 -1.3 1,411,364 + ydaQ b1346 0.48 -0.3 1,411,483 + +0.3 1,411,483 + -1.3 1,411,534 + -1.3 1,411,637 - ydaC b1347 0.39 +0.3 1,411,696 - ydaC b1347 0.67 +0.3 1,411,844 - lar b1348 0.45 +0.7 1,411,855 - lar b1348 0.50 -0.1 1,411,865 - lar b1348 0.55 -0.7 1,411,865 - lar b1348 0.55 -0.7 1,411,867 - lar b1348 0.56 -1.1 1,411,880 - lar b1348 0.63 +0.3 1,411,880 - lar b1348 0.63 -1.7 1,412,066 - -0.4 1,412,069 - +0.6 1,412,069 - +0.1 1,412,080 - +0.5 1,412,090 - recT b1349 0.10 -0.4 1,412,103 - recT b1349 0.12 +0.1 1,412,103 - recT b1349 0.12 +0.0 1,412,113 - recT b1349 0.13 +0.2 1,412,192 - recT b1349 0.23 +0.3 1,412,213 - recT b1349 0.25 -0.1 1,412,213 - recT b1349 0.25 +1.3 1,412,235 - recT b1349 0.28 -0.3 1,412,302 - recT b1349 0.36 -0.2 1,412,302 - recT b1349 0.36 +1.1 1,412,538 - recT b1349 0.65 -1.3 1,412,896 + +1.3 1,412,896 + -1.3 1,412,896 - -1.1 1,412,896 - -0.3
Or see this region's nucleotide sequence