Strain Fitness in Escherichia coli BW25113 around b1164

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycgF and ycgZ are separated by 313 nucleotidesycgZ and ymgA are separated by 42 nucleotidesymgA and ymgB are separated by 28 nucleotidesymgB and ymgC are separated by 112 nucleotides b1163: ycgF - predicted FAD-binding phosphodiesterase (NCBI), at 1,213,487 to 1,214,698 ycgF b1164: ycgZ - hypothetical protein (NCBI), at 1,215,012 to 1,215,248 ycgZ b1165: ymgA - hypothetical protein (NCBI), at 1,215,291 to 1,215,563 ymgA b1166: ymgB - hypothetical protein (NCBI), at 1,215,592 to 1,215,858 ymgB b1167: ymgC - hypothetical protein (NCBI), at 1,215,971 to 1,216,219 ymgC Position (kb) 1215 1216Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1214.067 kb on + strand, within ycgFat 1214.067 kb on + strand, within ycgFat 1214.151 kb on - strand, within ycgFat 1214.190 kb on + strand, within ycgFat 1214.190 kb on + strand, within ycgFat 1214.194 kb on - strand, within ycgFat 1214.203 kb on + strand, within ycgFat 1214.203 kb on + strand, within ycgFat 1214.203 kb on + strand, within ycgFat 1214.257 kb on + strand, within ycgFat 1214.366 kb on + strand, within ycgFat 1214.460 kb on + strand, within ycgFat 1214.460 kb on - strand, within ycgFat 1214.478 kb on + strand, within ycgFat 1214.541 kb on - strand, within ycgFat 1214.575 kb on + strand, within ycgFat 1214.590 kb on - strandat 1214.596 kb on - strandat 1214.612 kb on - strandat 1214.614 kb on + strandat 1214.614 kb on + strandat 1214.617 kb on - strandat 1214.718 kb on - strandat 1214.718 kb on - strandat 1214.730 kb on - strandat 1214.730 kb on - strandat 1214.730 kb on - strandat 1214.789 kb on + strandat 1215.133 kb on + strand, within ycgZat 1215.188 kb on + strand, within ycgZat 1215.190 kb on + strand, within ycgZat 1215.288 kb on + strandat 1215.346 kb on + strand, within ymgAat 1215.347 kb on + strand, within ymgAat 1215.349 kb on + strand, within ymgAat 1216.034 kb on + strand, within ymgCat 1216.162 kb on + strand, within ymgCat 1216.213 kb on + strandat 1216.243 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,214,067 + ycgF b1163 0.48 +0.0
1,214,067 + ycgF b1163 0.48 +0.3
1,214,151 - ycgF b1163 0.55 +0.3
1,214,190 + ycgF b1163 0.58 +1.9
1,214,190 + ycgF b1163 0.58 -1.2
1,214,194 - ycgF b1163 0.58 +0.3
1,214,203 + ycgF b1163 0.59 -0.7
1,214,203 + ycgF b1163 0.59 +0.3
1,214,203 + ycgF b1163 0.59 +0.3
1,214,257 + ycgF b1163 0.64 +1.3
1,214,366 + ycgF b1163 0.73 +0.3
1,214,460 + ycgF b1163 0.80 -0.7
1,214,460 - ycgF b1163 0.80 +0.3
1,214,478 + ycgF b1163 0.82 -0.7
1,214,541 - ycgF b1163 0.87 -1.7
1,214,575 + ycgF b1163 0.90 +0.3
1,214,590 - +0.3
1,214,596 - -1.2
1,214,612 - -3.0
1,214,614 + -0.7
1,214,614 + -1.2
1,214,617 - -1.7
1,214,718 - +0.3
1,214,718 - -0.7
1,214,730 - -2.0
1,214,730 - +1.3
1,214,730 - +0.3
1,214,789 + +0.3
1,215,133 + ycgZ b1164 0.51 +0.3
1,215,188 + ycgZ b1164 0.74 -0.7
1,215,190 + ycgZ b1164 0.75 -0.7
1,215,288 + +0.3
1,215,346 + ymgA b1165 0.20 +0.3
1,215,347 + ymgA b1165 0.21 +0.3
1,215,349 + ymgA b1165 0.21 -0.7
1,216,034 + ymgC b1167 0.25 +0.3
1,216,162 + ymgC b1167 0.77 +0.3
1,216,213 + +0.3
1,216,243 + +0.3

Or see this region's nucleotide sequence