Strain Fitness in Escherichia coli BW25113 around b1161

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntelbA and ycgX are separated by 699 nucleotidesycgX and ycgE are separated by 220 nucleotides b1160: elbA - hypothetical protein (NCBI), at 1,210,903 to 1,211,226 elbA b1161: ycgX - hypothetical protein (NCBI), at 1,211,926 to 1,212,330 ycgX b1162: ycgE - predicted DNA-binding transcriptional regulator (NCBI), at 1,212,551 to 1,213,282 ycgE Position (kb) 1211 1212 1213Strain fitness (log2 ratio) -2 -1 0 1 2at 1210.982 kb on - strand, within elbAat 1210.987 kb on - strand, within elbAat 1212.255 kb on - strand, within ycgXat 1212.292 kb on - strandat 1212.292 kb on - strandat 1212.327 kb on - strandat 1212.328 kb on - strandat 1212.345 kb on - strandat 1212.371 kb on - strandat 1212.371 kb on - strandat 1212.376 kb on - strandat 1212.376 kb on - strandat 1212.425 kb on - strandat 1212.427 kb on + strandat 1212.435 kb on - strandat 1212.435 kb on - strandat 1212.472 kb on + strandat 1212.477 kb on + strandat 1212.477 kb on + strandat 1212.483 kb on + strandat 1212.483 kb on + strandat 1212.579 kb on - strandat 1212.579 kb on - strandat 1212.596 kb on - strandat 1212.618 kb on + strandat 1212.689 kb on - strand, within ycgEat 1212.689 kb on - strand, within ycgEat 1212.803 kb on + strand, within ycgEat 1212.864 kb on - strand, within ycgEat 1212.892 kb on - strand, within ycgEat 1212.894 kb on + strand, within ycgEat 1212.951 kb on - strand, within ycgEat 1212.954 kb on + strand, within ycgEat 1212.993 kb on + strand, within ycgEat 1213.017 kb on - strand, within ycgEat 1213.051 kb on - strand, within ycgEat 1213.051 kb on - strand, within ycgEat 1213.160 kb on - strand, within ycgEat 1213.171 kb on + strand, within ycgEat 1213.247 kb on + strandat 1213.265 kb on - strandat 1213.265 kb on - strandat 1213.277 kb on - strandat 1213.301 kb on + strandat 1213.314 kb on + strandat 1213.314 kb on + strandat 1213.314 kb on + strandat 1213.314 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,210,982 - elbA b1160 0.24 +2.7
1,210,987 - elbA b1160 0.26 +0.3
1,212,255 - ycgX b1161 0.81 -0.7
1,212,292 - -0.7
1,212,292 - +0.3
1,212,327 - -0.3
1,212,328 - -1.8
1,212,345 - +0.8
1,212,371 - +1.1
1,212,371 - +0.1
1,212,376 - +0.3
1,212,376 - +0.9
1,212,425 - +0.3
1,212,427 + +0.4
1,212,435 - -2.2
1,212,435 - -1.1
1,212,472 + -2.2
1,212,477 + -0.7
1,212,477 + +0.3
1,212,483 + +0.3
1,212,483 + -0.2
1,212,579 - +0.3
1,212,579 - -0.4
1,212,596 - +0.3
1,212,618 + +0.3
1,212,689 - ycgE b1162 0.19 -0.7
1,212,689 - ycgE b1162 0.19 +0.3
1,212,803 + ycgE b1162 0.34 -0.4
1,212,864 - ycgE b1162 0.43 -0.6
1,212,892 - ycgE b1162 0.47 -0.3
1,212,894 + ycgE b1162 0.47 -0.2
1,212,951 - ycgE b1162 0.55 +0.2
1,212,954 + ycgE b1162 0.55 -0.2
1,212,993 + ycgE b1162 0.60 +0.3
1,213,017 - ycgE b1162 0.64 -2.5
1,213,051 - ycgE b1162 0.68 +1.0
1,213,051 - ycgE b1162 0.68 -0.3
1,213,160 - ycgE b1162 0.83 -0.1
1,213,171 + ycgE b1162 0.85 +2.2
1,213,247 + +0.9
1,213,265 - +0.3
1,213,265 - +0.3
1,213,277 - +0.3
1,213,301 + +0.5
1,213,314 + +1.3
1,213,314 + +0.1
1,213,314 + +0.3
1,213,314 + -0.1

Or see this region's nucleotide sequence