Strain Fitness in Escherichia coli BW25113 around b1105

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfF and ycfL are separated by 2 nucleotidesycfL and ycfM are separated by 13 nucleotidesycfM and ycfN overlap by 20 nucleotidesycfN and nagZ are separated by 10 nucleotides b1103: ycfF - orf, hypothetical protein (VIMSS), at 1,161,108 to 1,161,467 ycfF b1104: ycfL - hypothetical protein (NCBI), at 1,161,470 to 1,161,847 ycfL b1105: ycfM - predicted outer membrane lipoprotein (NCBI), at 1,161,861 to 1,162,502 ycfM b1106: ycfN - thiamin kinase (NCBI), at 1,162,483 to 1,163,307 ycfN b1107: nagZ - beta-hexosaminidase (NCBI), at 1,163,318 to 1,164,343 nagZ Position (kb) 1161 1162 1163Strain fitness (log2 ratio) -2 -1 0 1 2at 1161.023 kb on + strandat 1161.023 kb on + strandat 1161.034 kb on + strandat 1161.048 kb on - strandat 1161.048 kb on - strandat 1161.180 kb on - strand, within ycfFat 1161.414 kb on - strand, within ycfFat 1161.508 kb on - strand, within ycfLat 1161.648 kb on - strand, within ycfLat 1161.659 kb on + strand, within ycfLat 1161.996 kb on + strand, within ycfMat 1162.014 kb on + strand, within ycfMat 1162.014 kb on + strand, within ycfMat 1162.190 kb on - strand, within ycfMat 1162.347 kb on - strand, within ycfMat 1162.456 kb on - strandat 1162.640 kb on + strand, within ycfNat 1162.873 kb on - strand, within ycfNat 1162.875 kb on + strand, within ycfNat 1162.992 kb on - strand, within ycfNat 1163.024 kb on + strand, within ycfNat 1163.057 kb on - strand, within ycfNat 1163.213 kb on - strand, within ycfNat 1163.219 kb on + strand, within ycfNat 1163.220 kb on + strand, within ycfNat 1163.313 kb on + strandat 1163.324 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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1,161,023 + +1.6
1,161,023 + +2.1
1,161,034 + +0.3
1,161,048 - -0.4
1,161,048 - -1.2
1,161,180 - ycfF b1103 0.20 -0.1
1,161,414 - ycfF b1103 0.85 -1.7
1,161,508 - ycfL b1104 0.10 +0.1
1,161,648 - ycfL b1104 0.47 +1.3
1,161,659 + ycfL b1104 0.50 -0.5
1,161,996 + ycfM b1105 0.21 -1.2
1,162,014 + ycfM b1105 0.24 -1.8
1,162,014 + ycfM b1105 0.24 +0.2
1,162,190 - ycfM b1105 0.51 -0.6
1,162,347 - ycfM b1105 0.76 -1.4
1,162,456 - -0.1
1,162,640 + ycfN b1106 0.19 +0.1
1,162,873 - ycfN b1106 0.47 -1.7
1,162,875 + ycfN b1106 0.48 -0.8
1,162,992 - ycfN b1106 0.62 +0.4
1,163,024 + ycfN b1106 0.66 +0.6
1,163,057 - ycfN b1106 0.70 +0.3
1,163,213 - ycfN b1106 0.88 -0.1
1,163,219 + ycfN b1106 0.89 +0.3
1,163,220 + ycfN b1106 0.89 -0.1
1,163,313 + -1.5
1,163,324 - +0.9

Or see this region's nucleotide sequence