Strain Fitness in Escherichia coli BW25113 around b0741

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolB and pal are separated by 34 nucleotidespal and ybgF are separated by 9 nucleotidesybgF and lysT are separated by 164 nucleotides b0740: tolB - translocation protein TolB precursor (NCBI), at 776,963 to 778,255 tolB b0741: pal - peptidoglycan-associated outer membrane lipoprotein (NCBI), at 778,290 to 778,811 pal b0742: ybgF - hypothetical protein (NCBI), at 778,821 to 779,612 ybgF b0743: lysT - tRNA-Lys (NCBI), at 779,777 to 779,852 lysT Position (kb) 778 779Strain fitness (log2 ratio) -2 -1 0 1at 777.299 kb on - strand, within tolBat 777.303 kb on - strand, within tolBat 777.393 kb on - strand, within tolBat 777.398 kb on - strand, within tolBat 777.444 kb on - strand, within tolBat 777.513 kb on + strand, within tolBat 777.522 kb on - strand, within tolBat 777.602 kb on - strand, within tolBat 777.602 kb on - strand, within tolBat 777.692 kb on + strand, within tolBat 777.700 kb on - strand, within tolBat 777.720 kb on - strand, within tolBat 777.770 kb on - strand, within tolBat 777.792 kb on - strand, within tolBat 777.839 kb on - strand, within tolBat 777.952 kb on - strand, within tolBat 777.954 kb on - strand, within tolBat 777.987 kb on - strand, within tolBat 778.009 kb on - strand, within tolBat 778.014 kb on - strand, within tolBat 778.020 kb on - strand, within tolBat 778.070 kb on + strand, within tolBat 778.127 kb on - strandat 778.150 kb on - strandat 778.150 kb on - strandat 778.212 kb on + strandat 778.212 kb on + strandat 778.212 kb on + strandat 778.220 kb on - strandat 778.222 kb on + strandat 778.316 kb on + strandat 778.316 kb on + strandat 778.382 kb on + strand, within palat 778.395 kb on - strand, within palat 778.420 kb on - strand, within palat 778.422 kb on - strand, within palat 778.455 kb on + strand, within palat 778.455 kb on + strand, within palat 778.560 kb on + strand, within palat 778.568 kb on - strand, within palat 778.596 kb on - strand, within palat 778.600 kb on + strand, within palat 778.604 kb on - strand, within palat 778.656 kb on - strand, within palat 778.656 kb on - strandat 778.661 kb on - strand, within palat 778.713 kb on - strand, within palat 778.752 kb on + strand, within palat 778.752 kb on + strand, within palat 778.752 kb on + strand, within palat 778.780 kb on + strandat 778.780 kb on + strandat 778.805 kb on - strandat 778.851 kb on + strandat 778.856 kb on + strandat 778.864 kb on - strandat 778.870 kb on + strandat 778.932 kb on + strand, within ybgFat 778.940 kb on - strand, within ybgFat 778.940 kb on - strand, within ybgFat 778.940 kb on - strand, within ybgFat 778.940 kb on - strand, within ybgFat 779.036 kb on + strand, within ybgFat 779.145 kb on + strand, within ybgFat 779.145 kb on + strand, within ybgFat 779.158 kb on - strand, within ybgFat 779.196 kb on + strand, within ybgFat 779.201 kb on + strand, within ybgFat 779.201 kb on + strand, within ybgFat 779.204 kb on - strand, within ybgFat 779.263 kb on - strand, within ybgFat 779.263 kb on - strand, within ybgFat 779.269 kb on + strand, within ybgFat 779.342 kb on + strand, within ybgFat 779.342 kb on + strand, within ybgFat 779.392 kb on - strand, within ybgFat 779.428 kb on - strand, within ybgFat 779.510 kb on + strand, within ybgFat 779.544 kb on - strandat 779.544 kb on - strandat 779.565 kb on + strandat 779.610 kb on - strandat 779.619 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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777,299 - tolB b0740 0.26 -0.1
777,303 - tolB b0740 0.26 +0.5
777,393 - tolB b0740 0.33 +0.5
777,398 - tolB b0740 0.34 -0.5
777,444 - tolB b0740 0.37 -1.1
777,513 + tolB b0740 0.43 +0.5
777,522 - tolB b0740 0.43 +0.5
777,602 - tolB b0740 0.49 -1.1
777,602 - tolB b0740 0.49 -0.3
777,692 + tolB b0740 0.56 +1.5
777,700 - tolB b0740 0.57 +0.5
777,720 - tolB b0740 0.59 -0.5
777,770 - tolB b0740 0.62 -0.1
777,792 - tolB b0740 0.64 -1.1
777,839 - tolB b0740 0.68 +0.5
777,952 - tolB b0740 0.76 -0.1
777,954 - tolB b0740 0.77 +0.5
777,987 - tolB b0740 0.79 -0.1
778,009 - tolB b0740 0.81 +0.5
778,014 - tolB b0740 0.81 -0.5
778,020 - tolB b0740 0.82 -1.1
778,070 + tolB b0740 0.86 -1.5
778,127 - +0.5
778,150 - -0.8
778,150 - -2.1
778,212 + -1.1
778,212 + -0.5
778,212 + +0.5
778,220 - -0.5
778,222 + -0.5
778,316 + +0.5
778,316 + +0.7
778,382 + pal b0741 0.18 +0.5
778,395 - pal b0741 0.20 +0.5
778,420 - pal b0741 0.25 -0.5
778,422 - pal b0741 0.25 -0.5
778,455 + pal b0741 0.32 +0.5
778,455 + pal b0741 0.32 +0.5
778,560 + pal b0741 0.52 -1.5
778,568 - pal b0741 0.53 -0.8
778,596 - pal b0741 0.59 -1.5
778,600 + pal b0741 0.59 +0.5
778,604 - pal b0741 0.60 -0.5
778,656 - pal b0741 0.70 -0.5
778,656 - -0.5
778,661 - pal b0741 0.71 -0.3
778,713 - pal b0741 0.81 -1.1
778,752 + pal b0741 0.89 -0.3
778,752 + pal b0741 0.89 -0.5
778,752 + pal b0741 0.89 -2.3
778,780 + -2.0
778,780 + +0.5
778,805 - -1.3
778,851 + -0.2
778,856 + +0.1
778,864 - +0.5
778,870 + -0.4
778,932 + ybgF b0742 0.14 -0.5
778,940 - ybgF b0742 0.15 -0.4
778,940 - ybgF b0742 0.15 +1.6
778,940 - ybgF b0742 0.15 -0.5
778,940 - ybgF b0742 0.15 -2.1
779,036 + ybgF b0742 0.27 +0.4
779,145 + ybgF b0742 0.41 +1.2
779,145 + ybgF b0742 0.41 -2.0
779,158 - ybgF b0742 0.43 +0.2
779,196 + ybgF b0742 0.47 +0.7
779,201 + ybgF b0742 0.48 +1.5
779,201 + ybgF b0742 0.48 +0.5
779,204 - ybgF b0742 0.48 +0.1
779,263 - ybgF b0742 0.56 +0.5
779,263 - ybgF b0742 0.56 -0.0
779,269 + ybgF b0742 0.57 -0.2
779,342 + ybgF b0742 0.66 -1.3
779,342 + ybgF b0742 0.66 -1.8
779,392 - ybgF b0742 0.72 +1.6
779,428 - ybgF b0742 0.77 -1.3
779,510 + ybgF b0742 0.87 -0.1
779,544 - +0.8
779,544 - +0.1
779,565 + +1.0
779,610 - +0.9
779,619 + +0.1

Or see this region's nucleotide sequence