Experiment: LB Aerobic with Hygromycin B 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybfL and ybfD are separated by 130 nucleotides ybfD and ybgA are separated by 147 nucleotides ybgA and phr overlap by 4 nucleotides
b0705: ybfL - putative receptor protein (VIMSS), at 736,048 to 737,184
ybfL
b0706: ybfD - hypothetical protein (NCBI), at 737,315 to 738,076
ybfD
b0707: ybgA - hypothetical protein (NCBI), at 738,224 to 738,733
ybgA
b0708: phr - deoxyribodipyrimidine photolyase, FAD-binding (NCBI), at 738,730 to 740,148
phr
Position (kb) 737
738
739 Strain fitness (log2 ratio) -1
0
1 at 736.369 kb on - strand, within ybfL at 736.402 kb on - strand, within ybfL at 736.402 kb on - strand, within ybfL at 736.404 kb on - strand, within ybfL at 736.410 kb on + strand, within ybfL at 736.613 kb on + strand, within ybfL at 737.562 kb on - strand, within ybfD at 737.613 kb on - strand, within ybfD at 737.616 kb on - strand, within ybfD at 737.616 kb on - strand, within ybfD at 737.693 kb on - strand, within ybfD at 737.703 kb on - strand, within ybfD at 737.705 kb on - strand, within ybfD at 737.767 kb on + strand, within ybfD at 737.777 kb on + strand, within ybfD at 737.977 kb on - strand, within ybfD at 737.979 kb on - strand, within ybfD at 738.012 kb on - strand at 738.032 kb on - strand at 738.120 kb on - strand at 738.199 kb on - strand at 738.200 kb on + strand at 738.200 kb on + strand at 738.208 kb on - strand at 738.208 kb on - strand at 738.211 kb on + strand at 738.251 kb on + strand at 738.252 kb on + strand at 738.392 kb on - strand, within ybgA at 738.392 kb on - strand, within ybgA at 738.435 kb on + strand, within ybgA at 738.443 kb on - strand, within ybgA at 738.531 kb on - strand, within ybgA at 738.710 kb on - strand at 738.717 kb on + strand at 738.717 kb on + strand at 738.759 kb on + strand at 738.787 kb on - strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.837 kb on + strand at 738.844 kb on - strand at 738.844 kb on - strand at 738.844 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM remove 736,369 - ybfL b0705 0.28 +0.5 736,402 - ybfL b0705 0.31 +0.5 736,402 - ybfL b0705 0.31 +0.5 736,404 - ybfL b0705 0.31 +0.5 736,410 + ybfL b0705 0.32 +0.5 736,613 + ybfL b0705 0.50 +0.5 737,562 - ybfD b0706 0.32 +0.5 737,613 - ybfD b0706 0.39 +0.5 737,616 - ybfD b0706 0.40 -0.5 737,616 - ybfD b0706 0.40 +0.5 737,693 - ybfD b0706 0.50 +0.2 737,703 - ybfD b0706 0.51 +0.5 737,705 - ybfD b0706 0.51 +0.5 737,767 + ybfD b0706 0.59 -1.5 737,777 + ybfD b0706 0.61 -0.5 737,977 - ybfD b0706 0.87 -1.5 737,979 - ybfD b0706 0.87 +0.5 738,012 - -0.5 738,032 - -1.5 738,120 - +0.5 738,199 - +0.5 738,200 + -0.5 738,200 + +1.5 738,208 - -1.0 738,208 - +1.5 738,211 + +1.5 738,251 + +0.5 738,252 + +1.1 738,392 - ybgA b0707 0.33 +0.5 738,392 - ybgA b0707 0.33 +0.5 738,435 + ybgA b0707 0.41 +0.5 738,443 - ybgA b0707 0.43 -0.4 738,531 - ybgA b0707 0.60 -0.1 738,710 - -0.4 738,717 + -0.5 738,717 + -0.2 738,759 + -0.6 738,787 - +0.4 738,831 + -0.8 738,831 + -0.9 738,831 + +0.1 738,831 + +0.5 738,837 + +0.4 738,844 - +0.4 738,844 - +1.3 738,844 - -0.1
Or see this region's nucleotide sequence