Strain Fitness in Escherichia coli BW25113 around b0605

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdsbG and ahpC are separated by 371 nucleotidesahpC and ahpF are separated by 244 nucleotides b0604: dsbG - thiol:disulfide interchange protein (VIMSS), at 637,050 to 637,796 dsbG b0605: ahpC - alkyl hydroperoxide reductase, C22 subunit (NCBI), at 638,168 to 638,731 ahpC b0606: ahpF - alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS), at 638,976 to 640,541 ahpF Position (kb) 638 639Strain fitness (log2 ratio) -1 0 1at 637.185 kb on + strand, within dsbGat 637.204 kb on - strand, within dsbGat 637.204 kb on - strand, within dsbGat 637.217 kb on + strand, within dsbGat 637.237 kb on - strand, within dsbGat 637.237 kb on - strand, within dsbGat 637.244 kb on + strand, within dsbGat 637.498 kb on - strand, within dsbGat 637.836 kb on + strandat 637.944 kb on + strandat 637.944 kb on + strandat 637.969 kb on + strandat 638.144 kb on + strandat 638.144 kb on + strandat 638.315 kb on + strand, within ahpCat 638.376 kb on - strand, within ahpCat 638.378 kb on + strand, within ahpCat 638.489 kb on - strand, within ahpCat 638.513 kb on + strand, within ahpCat 638.513 kb on + strand, within ahpCat 638.557 kb on + strand, within ahpCat 638.557 kb on + strand, within ahpCat 638.557 kb on + strand, within ahpCat 638.625 kb on + strand, within ahpCat 638.692 kb on + strandat 638.923 kb on - strandat 638.923 kb on - strandat 638.937 kb on - strandat 639.002 kb on + strandat 639.227 kb on - strand, within ahpFat 639.227 kb on - strand, within ahpFat 639.268 kb on - strand, within ahpFat 639.428 kb on - strand, within ahpFat 639.448 kb on - strand, within ahpFat 639.639 kb on - strand, within ahpFat 639.681 kb on + strand, within ahpF

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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637,185 + dsbG b0604 0.18 -0.2
637,204 - dsbG b0604 0.21 +0.4
637,204 - dsbG b0604 0.21 -0.6
637,217 + dsbG b0604 0.22 -1.0
637,237 - dsbG b0604 0.25 +0.1
637,237 - dsbG b0604 0.25 +0.6
637,244 + dsbG b0604 0.26 -0.4
637,498 - dsbG b0604 0.60 +0.4
637,836 + +0.2
637,944 + +1.6
637,944 + +1.6
637,969 + +0.6
638,144 + +0.1
638,144 + +0.2
638,315 + ahpC b0605 0.26 -1.2
638,376 - ahpC b0605 0.37 -0.4
638,378 + ahpC b0605 0.37 -0.0
638,489 - ahpC b0605 0.57 -0.8
638,513 + ahpC b0605 0.61 -0.7
638,513 + ahpC b0605 0.61 +0.6
638,557 + ahpC b0605 0.69 +0.6
638,557 + ahpC b0605 0.69 -1.4
638,557 + ahpC b0605 0.69 -1.4
638,625 + ahpC b0605 0.81 -0.0
638,692 + -1.6
638,923 - -0.4
638,923 - -0.5
638,937 - +0.6
639,002 + -1.4
639,227 - ahpF b0606 0.16 -0.2
639,227 - ahpF b0606 0.16 -0.7
639,268 - ahpF b0606 0.19 +0.6
639,428 - ahpF b0606 0.29 -0.3
639,448 - ahpF b0606 0.30 +1.2
639,639 - ahpF b0606 0.42 -1.0
639,681 + ahpF b0606 0.45 -0.0

Or see this region's nucleotide sequence