Experiment: LB Aerobic with Hygromycin B 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgP and eaeH are separated by 551 nucleotides eaeH and insE are separated by 62 nucleotides insE and insF-1 overlap by 4 nucleotides
b4630: ykgP - no description, at 312,940 to 313,029
ykgP
b0297: eaeH - attaching and effacing protein, pathogenesis factor (RefSeq), at 313,581 to 314,452
eaeH
b0298: insE - IS3 element protein InsE (RefSeq), at 314,515 to 314,814
insE
b0299: insF-1 - IS3 element protein InsF (NCBI), at 314,811 to 315,677
insF-1
Position (kb)
313
314
315 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 312.586 kb on + strand at 312.596 kb on + strand at 312.605 kb on + strand at 312.615 kb on - strand at 312.619 kb on + strand at 312.642 kb on - strand at 312.648 kb on + strand at 312.648 kb on + strand at 312.650 kb on + strand at 312.658 kb on - strand at 312.660 kb on + strand at 312.660 kb on + strand at 312.664 kb on + strand at 312.666 kb on + strand at 312.672 kb on - strand at 312.674 kb on - strand at 312.677 kb on + strand at 312.679 kb on - strand at 312.679 kb on - strand at 312.690 kb on - strand at 312.690 kb on - strand at 312.693 kb on - strand at 312.695 kb on + strand at 312.712 kb on - strand at 312.721 kb on + strand at 312.721 kb on + strand at 312.721 kb on + strand at 312.729 kb on + strand at 312.731 kb on - strand at 312.743 kb on + strand at 312.766 kb on + strand at 312.784 kb on + strand at 312.785 kb on - strand at 312.800 kb on + strand at 312.800 kb on + strand at 312.800 kb on + strand at 312.802 kb on + strand at 312.805 kb on + strand at 312.810 kb on + strand at 312.816 kb on + strand at 312.818 kb on + strand at 312.822 kb on + strand at 312.822 kb on + strand at 312.829 kb on + strand at 312.831 kb on + strand at 312.831 kb on + strand at 312.837 kb on - strand at 312.837 kb on - strand at 312.839 kb on - strand at 312.841 kb on - strand at 312.841 kb on - strand at 312.841 kb on - strand at 312.854 kb on - strand at 312.854 kb on - strand at 312.968 kb on - strand, within ykgP at 312.973 kb on - strand, within ykgP at 312.973 kb on - strand, within ykgP at 313.025 kb on + strand at 313.078 kb on - strand at 313.120 kb on + strand at 313.126 kb on + strand at 313.131 kb on + strand at 313.136 kb on - strand at 313.141 kb on + strand at 313.231 kb on - strand at 313.451 kb on - strand at 313.597 kb on - strand at 313.597 kb on - strand at 313.597 kb on - strand at 313.600 kb on - strand at 313.600 kb on - strand at 313.637 kb on - strand at 313.637 kb on - strand at 313.660 kb on + strand at 313.695 kb on - strand, within eaeH at 313.697 kb on - strand, within eaeH at 313.704 kb on + strand, within eaeH at 313.716 kb on + strand, within eaeH at 313.725 kb on + strand, within eaeH at 313.726 kb on + strand, within eaeH at 313.732 kb on - strand, within eaeH at 313.735 kb on + strand, within eaeH at 313.735 kb on + strand, within eaeH at 313.735 kb on + strand, within eaeH at 313.735 kb on + strand, within eaeH at 313.768 kb on + strand, within eaeH at 313.841 kb on + strand, within eaeH at 314.025 kb on - strand, within eaeH at 314.040 kb on - strand, within eaeH at 314.053 kb on - strand, within eaeH at 314.157 kb on + strand, within eaeH at 314.157 kb on + strand, within eaeH at 314.166 kb on - strand, within eaeH at 314.166 kb on - strand, within eaeH at 314.169 kb on - strand, within eaeH at 314.223 kb on - strand, within eaeH at 314.223 kb on - strand, within eaeH at 314.229 kb on - strand, within eaeH at 314.265 kb on - strand, within eaeH at 314.276 kb on - strand, within eaeH at 314.297 kb on + strand, within eaeH at 314.297 kb on + strand, within eaeH at 314.324 kb on - strand, within eaeH at 314.341 kb on - strand, within eaeH at 314.356 kb on - strand, within eaeH at 314.358 kb on - strand, within eaeH at 314.358 kb on - strand, within eaeH at 314.358 kb on - strand, within eaeH at 314.360 kb on + strand, within eaeH at 314.368 kb on - strand at 314.384 kb on + strand at 314.384 kb on + strand at 314.392 kb on - strand at 314.401 kb on - strand at 314.407 kb on - strand at 314.437 kb on + strand at 314.437 kb on + strand at 314.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM remove 312,586 + -0.3 312,596 + +1.3 312,605 + -0.3 312,615 - -2.1 312,619 + +0.7 312,642 - -0.3 312,648 + -1.8 312,648 + -0.3 312,650 + +0.7 312,658 - +0.7 312,660 + -0.8 312,660 + +0.0 312,664 + -1.6 312,666 + -1.3 312,672 - +1.7 312,674 - -1.3 312,677 + -0.1 312,679 - +0.7 312,679 - +1.1 312,690 - +0.7 312,690 - +0.7 312,693 - +0.7 312,695 + +0.4 312,712 - -0.3 312,721 + +0.2 312,721 + +0.0 312,721 + -1.6 312,729 + +0.7 312,731 - +0.7 312,743 + +0.7 312,766 + +0.7 312,784 + -0.8 312,785 - +0.7 312,800 + -0.8 312,800 + -0.3 312,800 + +0.7 312,802 + -1.1 312,805 + +0.7 312,810 + +0.7 312,816 + +0.7 312,818 + +0.7 312,822 + +0.7 312,822 + +0.7 312,829 + +0.7 312,831 + +0.7 312,831 + +0.7 312,837 - +0.7 312,837 - -1.8 312,839 - +0.7 312,841 - +0.7 312,841 - +0.7 312,841 - -0.3 312,854 - +0.7 312,854 - +0.7 312,968 - ykgP b4630 0.31 +0.1 312,973 - ykgP b4630 0.37 +0.2 312,973 - ykgP b4630 0.37 -0.6 313,025 + +1.7 313,078 - -0.6 313,120 + +0.7 313,126 + +0.7 313,131 + +0.7 313,136 - -0.3 313,141 + +0.7 313,231 - -1.3 313,451 - +0.7 313,597 - -0.3 313,597 - +0.7 313,597 - -1.3 313,600 - -0.3 313,600 - +0.1 313,637 - -0.9 313,637 - -0.6 313,660 + +0.7 313,695 - eaeH b0297 0.13 +0.7 313,697 - eaeH b0297 0.13 -1.5 313,704 + eaeH b0297 0.14 +0.7 313,716 + eaeH b0297 0.15 -0.9 313,725 + eaeH b0297 0.17 +0.7 313,726 + eaeH b0297 0.17 +0.7 313,732 - eaeH b0297 0.17 -0.3 313,735 + eaeH b0297 0.18 +0.7 313,735 + eaeH b0297 0.18 -0.9 313,735 + eaeH b0297 0.18 -0.3 313,735 + eaeH b0297 0.18 -0.3 313,768 + eaeH b0297 0.21 -2.4 313,841 + eaeH b0297 0.30 +0.7 314,025 - eaeH b0297 0.51 -0.3 314,040 - eaeH b0297 0.53 -0.9 314,053 - eaeH b0297 0.54 -2.9 314,157 + eaeH b0297 0.66 +0.7 314,157 + eaeH b0297 0.66 +0.7 314,166 - eaeH b0297 0.67 +0.1 314,166 - eaeH b0297 0.67 -0.5 314,169 - eaeH b0297 0.67 -0.3 314,223 - eaeH b0297 0.74 +0.7 314,223 - eaeH b0297 0.74 +1.7 314,229 - eaeH b0297 0.74 -0.3 314,265 - eaeH b0297 0.78 -0.3 314,276 - eaeH b0297 0.80 -1.5 314,297 + eaeH b0297 0.82 -0.3 314,297 + eaeH b0297 0.82 -0.3 314,324 - eaeH b0297 0.85 -0.1 314,341 - eaeH b0297 0.87 -0.6 314,356 - eaeH b0297 0.89 +0.5 314,358 - eaeH b0297 0.89 -0.3 314,358 - eaeH b0297 0.89 +0.7 314,358 - eaeH b0297 0.89 +0.1 314,360 + eaeH b0297 0.89 +1.3 314,368 - -1.1 314,384 + -0.6 314,384 + +1.3 314,392 - -1.1 314,401 - -1.0 314,407 - -0.4 314,437 + -0.3 314,437 + +0.7 314,507 - +0.7
Or see this region's nucleotide sequence