Strain Fitness in Escherichia coli BW25113 around b0142

Experiment: LB Aerobic with Hygromycin B 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyadN and folK are separated by 369 nucleotidesfolK and pcnB overlap by 4 nucleotides b0141: yadN - predicted fimbrial-like adhesin protein (NCBI), at 156,299 to 156,883 yadN b0142: folK - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI), at 157,253 to 157,732 folK b0143: pcnB - poly(A) polymerase I (VIMSS), at 157,729 to 159,126 pcnB Position (kb) 157 158Strain fitness (log2 ratio) -2 -1 0 1 2at 156.298 kb on - strandat 156.298 kb on - strandat 156.298 kb on - strandat 156.298 kb on - strandat 156.298 kb on - strandat 156.298 kb on - strandat 156.298 kb on - strandat 156.312 kb on - strandat 156.322 kb on - strandat 156.322 kb on - strandat 156.322 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.324 kb on - strandat 156.332 kb on - strandat 156.332 kb on - strandat 156.356 kb on - strandat 156.356 kb on - strandat 156.356 kb on - strandat 156.356 kb on - strandat 156.359 kb on - strand, within yadNat 156.361 kb on - strand, within yadNat 156.367 kb on - strand, within yadNat 156.376 kb on - strand, within yadNat 156.376 kb on - strand, within yadNat 156.427 kb on - strand, within yadNat 156.428 kb on - strand, within yadNat 156.428 kb on - strand, within yadNat 156.429 kb on + strand, within yadNat 156.429 kb on + strand, within yadNat 156.431 kb on + strand, within yadNat 156.431 kb on + strand, within yadNat 156.432 kb on + strand, within yadNat 156.432 kb on + strand, within yadNat 156.437 kb on - strand, within yadNat 156.437 kb on - strand, within yadNat 156.437 kb on - strand, within yadNat 156.437 kb on - strand, within yadNat 156.437 kb on - strand, within yadNat 156.437 kb on - strand, within yadNat 156.437 kb on - strand, within yadNat 156.439 kb on - strand, within yadNat 156.439 kb on - strand, within yadNat 156.439 kb on - strand, within yadNat 156.439 kb on - strand, within yadNat 156.439 kb on - strand, within yadNat 156.440 kb on - strand, within yadNat 156.486 kb on + strand, within yadNat 156.489 kb on - strand, within yadNat 156.489 kb on - strand, within yadNat 156.521 kb on - strand, within yadNat 156.533 kb on - strand, within yadNat 156.545 kb on - strand, within yadNat 156.547 kb on - strand, within yadNat 156.560 kb on - strand, within yadNat 156.600 kb on + strand, within yadNat 156.602 kb on + strand, within yadNat 156.605 kb on - strand, within yadNat 156.642 kb on + strand, within yadNat 156.661 kb on + strand, within yadNat 156.682 kb on - strand, within yadNat 156.682 kb on - strand, within yadNat 156.685 kb on - strand, within yadNat 156.685 kb on - strand, within yadNat 156.685 kb on - strand, within yadNat 156.687 kb on - strand, within yadNat 156.705 kb on + strand, within yadNat 156.711 kb on + strand, within yadNat 156.721 kb on + strand, within yadNat 156.721 kb on + strand, within yadNat 156.721 kb on + strand, within yadNat 156.722 kb on + strand, within yadNat 156.734 kb on + strand, within yadNat 156.735 kb on + strand, within yadNat 156.735 kb on + strand, within yadNat 156.744 kb on + strand, within yadNat 156.755 kb on + strand, within yadNat 156.757 kb on + strand, within yadNat 156.759 kb on + strand, within yadNat 156.786 kb on + strand, within yadNat 156.789 kb on + strand, within yadNat 156.793 kb on + strand, within yadNat 156.804 kb on + strand, within yadNat 156.823 kb on + strand, within yadNat 156.823 kb on + strand, within yadNat 156.825 kb on + strandat 156.827 kb on + strandat 156.827 kb on + strandat 156.833 kb on - strandat 156.834 kb on + strandat 156.849 kb on + strandat 156.849 kb on + strandat 156.849 kb on + strandat 156.850 kb on + strandat 156.854 kb on + strandat 156.854 kb on + strandat 157.218 kb on - strandat 157.279 kb on - strandat 157.291 kb on - strandat 157.301 kb on - strand, within folKat 157.745 kb on + strandat 157.870 kb on - strand, within pcnBat 157.906 kb on - strand, within pcnBat 157.906 kb on - strand, within pcnBat 157.923 kb on - strand, within pcnBat 158.143 kb on + strand, within pcnBat 158.156 kb on + strand, within pcnBat 158.227 kb on - strand, within pcnBat 158.249 kb on - strand, within pcnBat 158.249 kb on - strand, within pcnBat 158.312 kb on - strand, within pcnBat 158.342 kb on - strand, within pcnBat 158.378 kb on - strand, within pcnBat 158.400 kb on + strand, within pcnBat 158.408 kb on - strand, within pcnBat 158.408 kb on - strand, within pcnBat 158.426 kb on - strand, within pcnBat 158.442 kb on - strand, within pcnBat 158.442 kb on - strand, within pcnBat 158.447 kb on - strand, within pcnBat 158.516 kb on + strand, within pcnBat 158.522 kb on - strand, within pcnB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM
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156,298 - +0.6
156,298 - +0.2
156,298 - +0.8
156,298 - +0.3
156,298 - +0.1
156,298 - +0.2
156,298 - +0.2
156,312 - -0.8
156,322 - +1.4
156,322 - -0.8
156,322 - +0.5
156,324 - +0.8
156,324 - -0.2
156,324 - +0.8
156,324 - +1.7
156,324 - -0.2
156,324 - -1.6
156,324 - +0.1
156,324 - +1.4
156,324 - +2.5
156,324 - +1.2
156,332 - +0.5
156,332 - -1.0
156,356 - -1.8
156,356 - +0.8
156,356 - +1.1
156,356 - -0.2
156,359 - yadN b0141 0.10 -2.7
156,361 - yadN b0141 0.11 -0.2
156,367 - yadN b0141 0.12 -0.5
156,376 - yadN b0141 0.13 +0.8
156,376 - yadN b0141 0.13 +0.8
156,427 - yadN b0141 0.22 -0.2
156,428 - yadN b0141 0.22 +0.8
156,428 - yadN b0141 0.22 +0.8
156,429 + yadN b0141 0.22 +0.8
156,429 + yadN b0141 0.22 +0.8
156,431 + yadN b0141 0.23 +0.8
156,431 + yadN b0141 0.23 +0.8
156,432 + yadN b0141 0.23 -0.2
156,432 + yadN b0141 0.23 +0.8
156,437 - yadN b0141 0.24 +0.8
156,437 - yadN b0141 0.24 +0.8
156,437 - yadN b0141 0.24 +1.8
156,437 - yadN b0141 0.24 +0.8
156,437 - yadN b0141 0.24 +0.8
156,437 - yadN b0141 0.24 -0.8
156,437 - yadN b0141 0.24 +0.1
156,439 - yadN b0141 0.24 +0.8
156,439 - yadN b0141 0.24 -0.2
156,439 - yadN b0141 0.24 -1.2
156,439 - yadN b0141 0.24 -0.8
156,439 - yadN b0141 0.24 -0.2
156,440 - yadN b0141 0.24 +0.8
156,486 + yadN b0141 0.32 +0.8
156,489 - yadN b0141 0.32 +0.8
156,489 - yadN b0141 0.32 +0.8
156,521 - yadN b0141 0.38 -1.0
156,533 - yadN b0141 0.40 -0.0
156,545 - yadN b0141 0.42 -0.8
156,547 - yadN b0141 0.42 +0.8
156,560 - yadN b0141 0.45 -0.2
156,600 + yadN b0141 0.51 +0.8
156,602 + yadN b0141 0.52 +0.8
156,605 - yadN b0141 0.52 -0.1
156,642 + yadN b0141 0.59 +0.8
156,661 + yadN b0141 0.62 +0.8
156,682 - yadN b0141 0.65 +1.5
156,682 - yadN b0141 0.65 -0.8
156,685 - yadN b0141 0.66 +0.8
156,685 - yadN b0141 0.66 -0.8
156,685 - yadN b0141 0.66 +0.8
156,687 - yadN b0141 0.66 +0.8
156,705 + yadN b0141 0.69 -1.8
156,711 + yadN b0141 0.70 +0.8
156,721 + yadN b0141 0.72 -0.2
156,721 + yadN b0141 0.72 +0.8
156,721 + yadN b0141 0.72 -0.2
156,722 + yadN b0141 0.72 +0.8
156,734 + yadN b0141 0.74 -0.8
156,735 + yadN b0141 0.75 -1.5
156,735 + yadN b0141 0.75 -0.8
156,744 + yadN b0141 0.76 -0.2
156,755 + yadN b0141 0.78 +0.8
156,757 + yadN b0141 0.78 -0.2
156,759 + yadN b0141 0.79 -1.5
156,786 + yadN b0141 0.83 +0.8
156,789 + yadN b0141 0.84 +1.4
156,793 + yadN b0141 0.84 -1.2
156,804 + yadN b0141 0.86 -0.6
156,823 + yadN b0141 0.90 +1.8
156,823 + yadN b0141 0.90 -0.2
156,825 + -0.4
156,827 + -0.5
156,827 + +1.1
156,833 - +0.8
156,834 + +0.8
156,849 + +1.8
156,849 + +0.4
156,849 + +0.8
156,850 + -1.5
156,854 + +0.8
156,854 + -1.2
157,218 - +0.8
157,279 - +1.8
157,291 - -1.2
157,301 - folK b0142 0.10 -1.5
157,745 + +0.3
157,870 - pcnB b0143 0.10 -0.4
157,906 - pcnB b0143 0.13 -0.5
157,906 - pcnB b0143 0.13 -0.5
157,923 - pcnB b0143 0.14 -0.7
158,143 + pcnB b0143 0.30 -1.0
158,156 + pcnB b0143 0.31 -0.1
158,227 - pcnB b0143 0.36 +0.3
158,249 - pcnB b0143 0.37 +0.0
158,249 - pcnB b0143 0.37 -1.1
158,312 - pcnB b0143 0.42 +0.3
158,342 - pcnB b0143 0.44 -1.2
158,378 - pcnB b0143 0.46 +0.1
158,400 + pcnB b0143 0.48 -1.4
158,408 - pcnB b0143 0.49 -0.5
158,408 - pcnB b0143 0.49 +0.8
158,426 - pcnB b0143 0.50 -0.4
158,442 - pcnB b0143 0.51 -0.2
158,442 - pcnB b0143 0.51 +1.5
158,447 - pcnB b0143 0.51 -1.1
158,516 + pcnB b0143 0.56 +0.6
158,522 - pcnB b0143 0.57 -0.0

Or see this region's nucleotide sequence