Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0625 and PP_t07 are separated by 362 nucleotides PP_t07 and PP_t08 are separated by 4 nucleotides PP_t08 and PP_t09 are separated by 6 nucleotides PP_t09 and PP_t10 are separated by 46 nucleotides PP_t10 and PP_t11 are separated by 5 nucleotides PP_t11 and PP_0626 are separated by 190 nucleotides
PP_0625: PP_0625 - Chaperone protein ClpB, at 730,461 to 733,025
_0625
PP_t07: PP_t07 - tRNA-Asn, at 733,388 to 733,463
_t07
PP_t08: PP_t08 - tRNA-Pro, at 733,468 to 733,544
_t08
PP_t09: PP_t09 - tRNA-Lys, at 733,551 to 733,626
_t09
PP_t10: PP_t10 - tRNA-Pro, at 733,673 to 733,749
_t10
PP_t11: PP_t11 - tRNA-Lys, at 733,755 to 733,830
_t11
PP_0626: PP_0626 - NADH dehydrogenase, at 734,021 to 735,316
_0626
Position (kb) 733
734 Strain fitness (log2 ratio) -1
0
1
2
3 at 732.777 kb on - strand at 732.889 kb on - strand at 733.179 kb on + strand at 733.181 kb on + strand at 733.181 kb on + strand at 733.181 kb on + strand at 733.475 kb on + strand at 733.475 kb on + strand at 733.629 kb on + strand at 733.681 kb on - strand, within PP_t10 at 733.681 kb on - strand, within PP_t10 at 733.763 kb on - strand, within PP_t11 at 733.763 kb on - strand, within PP_t11 at 733.775 kb on - strand, within PP_t11 at 733.775 kb on - strand, within PP_t11 at 733.775 kb on - strand, within PP_t11 at 733.912 kb on + strand at 733.913 kb on - strand at 733.913 kb on - strand at 733.930 kb on + strand at 733.931 kb on - strand at 733.931 kb on - strand at 733.934 kb on + strand at 733.934 kb on + strand at 733.934 kb on + strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.989 kb on - strand at 734.022 kb on + strand at 734.023 kb on - strand at 734.100 kb on + strand at 734.124 kb on + strand at 734.124 kb on + strand at 734.136 kb on + strand at 734.136 kb on + strand at 734.137 kb on - strand at 734.137 kb on - strand at 734.137 kb on - strand at 734.137 kb on - strand at 734.137 kb on - strand at 734.238 kb on + strand, within PP_0626 at 734.238 kb on + strand, within PP_0626 at 734.239 kb on - strand, within PP_0626 at 734.239 kb on - strand, within PP_0626 at 734.247 kb on + strand, within PP_0626 at 734.248 kb on - strand, within PP_0626 at 734.253 kb on + strand, within PP_0626 at 734.254 kb on - strand at 734.254 kb on - strand, within PP_0626 at 734.254 kb on - strand, within PP_0626 at 734.254 kb on - strand, within PP_0626 at 734.254 kb on - strand, within PP_0626 at 734.347 kb on + strand, within PP_0626 at 734.731 kb on - strand, within PP_0626 at 734.731 kb on - strand, within PP_0626
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 732,777 - -1.6 732,889 - +2.7 733,179 + +3.7 733,181 + +1.7 733,181 + +1.1 733,181 + +2.7 733,475 + +0.1 733,475 + +1.1 733,629 + +0.4 733,681 - PP_t10 0.10 -1.4 733,681 - PP_t10 0.10 -0.5 733,763 - PP_t11 0.11 +1.3 733,763 - PP_t11 0.11 +0.1 733,775 - PP_t11 0.26 +0.6 733,775 - PP_t11 0.26 +1.1 733,775 - PP_t11 0.26 -0.8 733,912 + +0.3 733,913 - -0.1 733,913 - -0.1 733,930 + -1.1 733,931 - -0.9 733,931 - -0.0 733,934 + -0.1 733,934 + -0.2 733,934 + -0.3 733,935 - -1.2 733,935 - -0.6 733,935 - -0.7 733,935 - -1.3 733,935 - +0.2 733,935 - +0.7 733,935 - -0.0 733,935 - +0.2 733,989 - -0.1 734,022 + +0.1 734,023 - -0.3 734,100 + +1.7 734,124 + -0.5 734,124 + -0.1 734,136 + -0.3 734,136 + -0.8 734,137 - +0.8 734,137 - -0.3 734,137 - -0.4 734,137 - -0.6 734,137 - +0.1 734,238 + PP_0626 0.17 -0.3 734,238 + PP_0626 0.17 -0.4 734,239 - PP_0626 0.17 +1.7 734,239 - PP_0626 0.17 +0.9 734,247 + PP_0626 0.17 +1.7 734,248 - PP_0626 0.18 -0.8 734,253 + PP_0626 0.18 +0.2 734,254 - +1.7 734,254 - PP_0626 0.18 -0.9 734,254 - PP_0626 0.18 +0.2 734,254 - PP_0626 0.18 -1.5 734,254 - PP_0626 0.18 -1.2 734,347 + PP_0626 0.25 -1.6 734,731 - PP_0626 0.55 +0.6 734,731 - PP_0626 0.55 +0.9
Or see this region's nucleotide sequence