Strain Fitness in Pseudomonas putida KT2440 around PP_5345

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
6,091,738	+		PP_5343	0.26	-0.1
6,091,738	+		PP_5343	0.26	-0.5
6,091,739	-		PP_5343	0.26	-0.6
6,091,739	-		PP_5343	0.26	-0.3
6,091,739	-		PP_5343	0.26	-0.6
6,091,739	-		PP_5343	0.26	+0.5
6,091,739	-		PP_5343	0.26	+0.4
6,091,739	-		PP_5343	0.26	+2.7
6,091,806	-		PP_5343	0.37	+0.2
6,091,825	+		PP_5343	0.40	+0.6
6,091,825	+		PP_5343	0.40	+0.3
6,091,825	+		PP_5343	0.40	+0.3
6,091,826	-		PP_5343	0.40	-2.0
6,091,840	+		PP_5343	0.42	-0.3
6,091,841	-		PP_5343	0.42	+1.3
6,091,841	-		PP_5343	0.42	+0.7
6,091,843	+		PP_5343	0.42	+0.1
6,091,844	-		PP_5343	0.43	+2.7
6,091,889	-		PP_5343	0.50	-0.2
6,091,906	+		PP_5343	0.52	-0.8
6,091,906	+		PP_5343	0.52	-1.7
6,091,907	-		PP_5343	0.53	-0.7
6,091,907	-		PP_5343	0.53	+1.9
6,091,924	-		PP_5343	0.55	+0.2
6,091,924	-		PP_5343	0.55	-0.4
6,092,027	-		PP_5343	0.72	-0.1
6,092,211	+				-0.1
6,092,212	-				-0.9
6,092,228	+				+0.3
6,092,228	+				+0.5
6,092,228	+				+2.7
6,092,229	-				-1.5
6,092,286	+		PP_5344	0.15	-0.2
6,092,286	+		PP_5344	0.15	+0.3
6,092,286	+		PP_5344	0.15	+0.4
6,092,286	+		PP_5344	0.15	+2.0
6,092,287	-		PP_5344	0.16	-0.2
6,092,299	-		PP_5344	0.18	+1.3
6,092,424	+		PP_5344	0.46	-0.9
6,092,424	+		PP_5344	0.46	-0.8
6,092,424	+		PP_5344	0.46	-0.4
6,092,425	-		PP_5344	0.47	-0.5
6,092,425	-		PP_5344	0.47	-0.2
6,092,460	+		PP_5344	0.54	-0.6
6,092,461	-		PP_5344	0.55	-0.7
6,092,475	+		PP_5344	0.58	+0.7
6,092,475	+		PP_5344	0.58	-1.0
6,092,475	+		PP_5344	0.58	+0.2
6,092,475	+		PP_5344	0.58	-0.4
6,092,475	+		PP_5344	0.58	+1.0
6,092,475	+		PP_5344	0.58	-0.4
6,092,476	-		PP_5344	0.58	-0.3
6,092,476	-		PP_5344	0.58	+0.7
6,092,476	-		PP_5344	0.58	+0.2
6,092,476	-		PP_5344	0.58	+0.2
6,092,476	-		PP_5344	0.58	+1.1
6,092,476	-		PP_5344	0.58	-0.3
6,092,476	-		PP_5344	0.58	+0.0
6,092,476	-		PP_5344	0.58	-2.3
6,092,476	-		PP_5344	0.58	-0.9
6,092,532	+		PP_5344	0.70	-0.7
6,092,532	+		PP_5344	0.70	-0.5
6,092,532	+		PP_5344	0.70	+0.1
6,092,532	+		PP_5344	0.70	+0.0
6,092,533	-		PP_5344	0.71	-0.4
6,092,533	-		PP_5344	0.71	+0.7
6,092,533	-		PP_5344	0.71	+1.1
6,092,662	-				+0.7
6,092,662	-				-1.7
6,092,662	-				-0.2
6,092,662	-				-0.2
6,092,827	+		PP_5345	0.23	-0.2
6,092,827	+		PP_5345	0.23	-1.1
6,092,827	+		PP_5345	0.23	+0.5
6,092,827	+		PP_5345	0.23	-1.7
6,092,912	-		PP_5345	0.36	-0.7
6,093,055	+		PP_5345	0.57	-1.3
6,093,056	-		PP_5345	0.57	-1.4
6,093,056	-		PP_5345	0.57	-0.0
6,093,205	-		PP_5345	0.79	-0.7
6,093,213	+		PP_5345	0.81	-1.1
6,093,364	+				-1.1
6,093,365	-				-0.1
6,093,365	-				+0.2
6,093,365	-				-0.8
6,093,446	+				-1.7
6,093,446	+				-1.1
6,093,447	-				-0.6
6,093,459	-				+2.7
6,093,537	-				-1.3
6,093,738	+		PP_5346	0.15	-2.0
6,093,739	-		PP_5346	0.15	-1.3
6,093,772	+		PP_5346	0.17	-0.5
6,093,772	+		PP_5346	0.17	-2.5
6,093,772	+		PP_5346	0.17	-1.7
6,093,840	+		PP_5346	0.21	+0.1
6,093,841	-		PP_5346	0.21	-0.7
6,094,034	+		PP_5346	0.32	-1.0
6,094,165	+		PP_5346	0.39	-2.2
6,094,165	+		PP_5346	0.39	-3.7
6,094,195	+		PP_5346	0.41	-2.1