Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5074 and PP_5075 are separated by 194 nucleotides PP_5075 and PP_5076 are separated by 123 nucleotides
PP_5074: PP_5074 - Uroporphyrinogen decarboxylase, at 5,790,653 to 5,791,717
_5074
PP_5075: PP_5075 - glutamate synthase (NADPH) beta subunit, at 5,791,912 to 5,793,330
_5075
PP_5076: PP_5076 - L-glutamate synthase(NADPH) alpha subunit, at 5,793,454 to 5,797,899
_5076
Position (kb) 5791
5792
5793
5794 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5791.828 kb on - strand at 5791.950 kb on + strand at 5791.951 kb on - strand at 5792.294 kb on + strand, within PP_5075 at 5792.295 kb on - strand, within PP_5075 at 5792.295 kb on - strand, within PP_5075 at 5792.329 kb on - strand, within PP_5075 at 5792.372 kb on + strand, within PP_5075 at 5792.373 kb on - strand, within PP_5075 at 5792.412 kb on + strand, within PP_5075 at 5792.412 kb on + strand, within PP_5075 at 5792.591 kb on + strand, within PP_5075 at 5792.591 kb on + strand, within PP_5075 at 5792.591 kb on + strand, within PP_5075 at 5792.591 kb on + strand, within PP_5075 at 5792.591 kb on + strand, within PP_5075 at 5792.592 kb on - strand, within PP_5075 at 5792.592 kb on - strand, within PP_5075 at 5792.592 kb on - strand, within PP_5075 at 5792.592 kb on - strand, within PP_5075 at 5792.592 kb on - strand, within PP_5075 at 5792.592 kb on - strand, within PP_5075 at 5792.592 kb on - strand, within PP_5075 at 5792.597 kb on + strand, within PP_5075 at 5792.597 kb on + strand, within PP_5075 at 5792.597 kb on + strand, within PP_5075 at 5792.597 kb on + strand, within PP_5075 at 5792.597 kb on + strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.598 kb on - strand, within PP_5075 at 5792.602 kb on - strand, within PP_5075 at 5792.602 kb on - strand, within PP_5075 at 5792.602 kb on - strand, within PP_5075 at 5792.627 kb on + strand, within PP_5075 at 5792.948 kb on + strand, within PP_5075 at 5792.948 kb on + strand, within PP_5075 at 5792.949 kb on - strand, within PP_5075 at 5792.949 kb on - strand, within PP_5075 at 5792.949 kb on - strand, within PP_5075 at 5792.949 kb on - strand, within PP_5075 at 5792.949 kb on - strand, within PP_5075 at 5792.949 kb on - strand, within PP_5075 at 5793.043 kb on + strand, within PP_5075 at 5793.043 kb on + strand, within PP_5075 at 5793.043 kb on + strand, within PP_5075 at 5793.043 kb on + strand, within PP_5075 at 5793.044 kb on - strand, within PP_5075 at 5793.044 kb on - strand, within PP_5075 at 5793.044 kb on - strand, within PP_5075 at 5793.162 kb on - strand, within PP_5075 at 5793.228 kb on - strand at 5793.346 kb on + strand at 5793.347 kb on - strand at 5793.347 kb on - strand at 5793.347 kb on - strand at 5793.362 kb on + strand at 5793.362 kb on + strand at 5793.363 kb on - strand at 5793.391 kb on + strand at 5793.391 kb on + strand at 5793.392 kb on - strand at 5793.536 kb on + strand at 5793.536 kb on + strand at 5793.536 kb on + strand at 5793.537 kb on - strand at 5793.593 kb on + strand at 5793.593 kb on + strand at 5793.594 kb on - strand at 5793.594 kb on - strand at 5793.594 kb on - strand at 5793.627 kb on - strand at 5793.627 kb on - strand at 5793.651 kb on + strand at 5793.652 kb on - strand at 5793.696 kb on + strand at 5793.696 kb on + strand at 5793.696 kb on + strand at 5793.696 kb on + strand at 5793.696 kb on + strand at 5793.696 kb on + strand at 5793.697 kb on - strand at 5793.697 kb on - strand at 5793.697 kb on - strand at 5793.697 kb on - strand at 5793.716 kb on + strand at 5793.716 kb on + strand at 5793.717 kb on - strand at 5793.717 kb on - strand at 5793.753 kb on - strand at 5793.794 kb on + strand at 5793.794 kb on + strand at 5793.795 kb on - strand at 5793.795 kb on - strand at 5793.795 kb on - strand at 5793.847 kb on - strand at 5793.847 kb on - strand at 5793.850 kb on + strand at 5793.851 kb on - strand at 5793.851 kb on - strand at 5793.908 kb on + strand, within PP_5076 at 5793.908 kb on + strand, within PP_5076 at 5793.908 kb on + strand, within PP_5076 at 5793.909 kb on - strand, within PP_5076 at 5794.010 kb on + strand, within PP_5076 at 5794.011 kb on - strand, within PP_5076 at 5794.242 kb on + strand, within PP_5076 at 5794.242 kb on + strand, within PP_5076 at 5794.242 kb on + strand, within PP_5076 at 5794.242 kb on + strand, within PP_5076
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,791,828 - -0.3 5,791,950 + -2.7 5,791,951 - -1.5 5,792,294 + PP_5075 0.27 -2.0 5,792,295 - PP_5075 0.27 -3.1 5,792,295 - PP_5075 0.27 -3.6 5,792,329 - PP_5075 0.29 -3.1 5,792,372 + PP_5075 0.32 -2.6 5,792,373 - PP_5075 0.32 -3.2 5,792,412 + PP_5075 0.35 +0.3 5,792,412 + PP_5075 0.35 -1.3 5,792,591 + PP_5075 0.48 -2.3 5,792,591 + PP_5075 0.48 -2.8 5,792,591 + PP_5075 0.48 -0.7 5,792,591 + PP_5075 0.48 -1.5 5,792,591 + PP_5075 0.48 -1.9 5,792,592 - PP_5075 0.48 -3.4 5,792,592 - PP_5075 0.48 -1.2 5,792,592 - PP_5075 0.48 +1.7 5,792,592 - PP_5075 0.48 -3.3 5,792,592 - PP_5075 0.48 +0.7 5,792,592 - PP_5075 0.48 -1.4 5,792,592 - PP_5075 0.48 -0.1 5,792,597 + PP_5075 0.48 +1.1 5,792,597 + PP_5075 0.48 -2.9 5,792,597 + PP_5075 0.48 -3.6 5,792,597 + PP_5075 0.48 -1.4 5,792,597 + PP_5075 0.48 -2.6 5,792,598 - PP_5075 0.48 -3.9 5,792,598 - PP_5075 0.48 -1.4 5,792,598 - PP_5075 0.48 -2.0 5,792,598 - PP_5075 0.48 -0.1 5,792,598 - PP_5075 0.48 -2.5 5,792,598 - PP_5075 0.48 -2.4 5,792,598 - PP_5075 0.48 -2.4 5,792,598 - PP_5075 0.48 -1.6 5,792,598 - PP_5075 0.48 -2.0 5,792,602 - PP_5075 0.49 +1.7 5,792,602 - PP_5075 0.49 -0.5 5,792,602 - PP_5075 0.49 +0.3 5,792,627 + PP_5075 0.50 +0.7 5,792,948 + PP_5075 0.73 -0.1 5,792,948 + PP_5075 0.73 -0.7 5,792,949 - PP_5075 0.73 -2.0 5,792,949 - PP_5075 0.73 -1.5 5,792,949 - PP_5075 0.73 -2.0 5,792,949 - PP_5075 0.73 -1.7 5,792,949 - PP_5075 0.73 -0.5 5,792,949 - PP_5075 0.73 -3.7 5,793,043 + PP_5075 0.80 -0.7 5,793,043 + PP_5075 0.80 -1.9 5,793,043 + PP_5075 0.80 -1.7 5,793,043 + PP_5075 0.80 -2.6 5,793,044 - PP_5075 0.80 -0.7 5,793,044 - PP_5075 0.80 -1.9 5,793,044 - PP_5075 0.80 -3.1 5,793,162 - PP_5075 0.88 -1.9 5,793,228 - -1.0 5,793,346 + -0.5 5,793,347 - +2.7 5,793,347 - +0.8 5,793,347 - -1.0 5,793,362 + -1.3 5,793,362 + -1.1 5,793,363 - -0.5 5,793,391 + -1.4 5,793,391 + -0.3 5,793,392 - +0.5 5,793,536 + -1.4 5,793,536 + -1.5 5,793,536 + -2.4 5,793,537 - +2.7 5,793,593 + -2.1 5,793,593 + -0.9 5,793,594 - -0.9 5,793,594 - -2.1 5,793,594 - -1.4 5,793,627 - -0.2 5,793,627 - -2.2 5,793,651 + -3.0 5,793,652 - -2.5 5,793,696 + -2.4 5,793,696 + +1.1 5,793,696 + -1.8 5,793,696 + -1.8 5,793,696 + -1.6 5,793,696 + +0.3 5,793,697 - -0.7 5,793,697 - -1.8 5,793,697 - -0.7 5,793,697 - +0.1 5,793,716 + -1.6 5,793,716 + -2.9 5,793,717 - -2.3 5,793,717 - +0.3 5,793,753 - -0.5 5,793,794 + -3.0 5,793,794 + -0.3 5,793,795 - -1.4 5,793,795 - +1.7 5,793,795 - -1.1 5,793,847 - -4.1 5,793,847 - -0.8 5,793,850 + -1.8 5,793,851 - -3.6 5,793,851 - -0.1 5,793,908 + PP_5076 0.10 -3.2 5,793,908 + PP_5076 0.10 -2.9 5,793,908 + PP_5076 0.10 -2.1 5,793,909 - PP_5076 0.10 +1.4 5,794,010 + PP_5076 0.13 -2.6 5,794,011 - PP_5076 0.13 -3.8 5,794,242 + PP_5076 0.18 -3.3 5,794,242 + PP_5076 0.18 -2.5 5,794,242 + PP_5076 0.18 -1.3 5,794,242 + PP_5076 0.18 -2.2
Or see this region's nucleotide sequence