Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5013 and PP_5014 are separated by 72 nucleotides PP_5014 and PP_5015 are separated by 1 nucleotides PP_5015 and PP_5016 are separated by 81 nucleotides PP_5016 and PP_5017 are separated by 3 nucleotides
PP_5013: PP_5013 - 2-octaprenylphenol hydroxylase, at 5,712,552 to 5,714,174
_5013
PP_5014: PP_5014 - Phosphoribosyl-AMP cyclohydrolase, at 5,714,247 to 5,714,639
_5014
PP_5015: PP_5015 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) <small>(from data)</small>, at 5,714,641 to 5,714,976
_5015
PP_5016: PP_5016 - twin arginine protein export complex subunit, at 5,715,058 to 5,715,330
_5016
PP_5017: PP_5017 - Sec-independent protein translocase protein TatB, at 5,715,334 to 5,715,711
_5017
Position (kb) 5714
5715 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5714.172 kb on + strand at 5714.200 kb on + strand at 5714.200 kb on + strand at 5714.395 kb on - strand, within PP_5014 at 5714.494 kb on + strand, within PP_5014 at 5714.523 kb on - strand, within PP_5014 at 5714.556 kb on + strand, within PP_5014 at 5714.557 kb on - strand, within PP_5014 at 5714.565 kb on + strand, within PP_5014 at 5714.566 kb on - strand, within PP_5014 at 5714.566 kb on - strand, within PP_5014 at 5714.622 kb on + strand at 5714.623 kb on - strand at 5714.623 kb on - strand at 5714.710 kb on + strand, within PP_5015 at 5714.710 kb on + strand, within PP_5015 at 5714.710 kb on + strand, within PP_5015 at 5714.710 kb on + strand, within PP_5015 at 5714.711 kb on - strand, within PP_5015 at 5714.711 kb on - strand, within PP_5015 at 5714.725 kb on + strand at 5714.818 kb on + strand, within PP_5015 at 5714.834 kb on - strand, within PP_5015 at 5715.026 kb on + strand at 5715.033 kb on + strand at 5715.564 kb on + strand, within PP_5017 at 5715.564 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,714,172 + +0.4 5,714,200 + -1.6 5,714,200 + +0.5 5,714,395 - PP_5014 0.38 +2.7 5,714,494 + PP_5014 0.63 -2.2 5,714,523 - PP_5014 0.70 +1.1 5,714,556 + PP_5014 0.79 -0.8 5,714,557 - PP_5014 0.79 -0.5 5,714,565 + PP_5014 0.81 -3.1 5,714,566 - PP_5014 0.81 +1.7 5,714,566 - PP_5014 0.81 +1.7 5,714,622 + +0.1 5,714,623 - +2.7 5,714,623 - +2.7 5,714,710 + PP_5015 0.21 -2.5 5,714,710 + PP_5015 0.21 +1.1 5,714,710 + PP_5015 0.21 -1.6 5,714,710 + PP_5015 0.21 -0.9 5,714,711 - PP_5015 0.21 +2.7 5,714,711 - PP_5015 0.21 +1.7 5,714,725 + +0.1 5,714,818 + PP_5015 0.53 -2.1 5,714,834 - PP_5015 0.57 +0.7 5,715,026 + +0.2 5,715,033 + -0.1 5,715,564 + PP_5017 0.61 +0.2 5,715,564 + +3.7
Or see this region's nucleotide sequence