Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4976 and PP_4977 are separated by 124 nucleotides PP_4977 and PP_4978 are separated by 84 nucleotides
PP_4976: PP_4976 - Adenosylhomocysteinase, at 5,668,005 to 5,669,399
_4976
PP_4977: PP_4977 - 5,10-methylenetetrahydrofolate reductase, at 5,669,524 to 5,670,411
_4977
PP_4978: PP_4978 - conserved protein of unknown function, at 5,670,496 to 5,671,581
_4978
Position (kb) 5669
5670
5671 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5669.397 kb on + strand at 5669.398 kb on - strand at 5669.398 kb on - strand at 5669.398 kb on - strand at 5669.398 kb on - strand at 5669.437 kb on + strand at 5669.437 kb on + strand at 5669.438 kb on - strand at 5669.438 kb on - strand at 5669.521 kb on + strand at 5669.521 kb on + strand at 5669.521 kb on + strand at 5669.662 kb on + strand, within PP_4977 at 5669.662 kb on + strand, within PP_4977 at 5669.662 kb on + strand, within PP_4977 at 5669.662 kb on + strand, within PP_4977 at 5669.662 kb on + strand, within PP_4977 at 5669.663 kb on - strand, within PP_4977 at 5669.663 kb on - strand, within PP_4977 at 5669.663 kb on - strand, within PP_4977 at 5669.663 kb on - strand, within PP_4977 at 5669.663 kb on - strand, within PP_4977 at 5669.663 kb on - strand, within PP_4977 at 5669.663 kb on - strand, within PP_4977 at 5669.664 kb on + strand, within PP_4977 at 5669.689 kb on + strand, within PP_4977 at 5669.689 kb on + strand, within PP_4977 at 5669.690 kb on - strand, within PP_4977 at 5669.690 kb on - strand, within PP_4977 at 5669.759 kb on + strand, within PP_4977 at 5669.759 kb on + strand, within PP_4977 at 5669.759 kb on + strand, within PP_4977 at 5669.760 kb on - strand, within PP_4977 at 5669.828 kb on - strand, within PP_4977 at 5669.911 kb on + strand, within PP_4977 at 5669.911 kb on + strand, within PP_4977 at 5669.912 kb on - strand, within PP_4977 at 5669.912 kb on - strand, within PP_4977 at 5669.912 kb on - strand, within PP_4977 at 5670.076 kb on + strand, within PP_4977 at 5670.077 kb on - strand, within PP_4977 at 5670.077 kb on - strand, within PP_4977 at 5670.097 kb on + strand, within PP_4977 at 5670.098 kb on - strand, within PP_4977 at 5670.098 kb on - strand, within PP_4977 at 5670.098 kb on - strand, within PP_4977 at 5670.098 kb on - strand, within PP_4977 at 5670.103 kb on + strand, within PP_4977 at 5670.103 kb on + strand, within PP_4977 at 5670.175 kb on + strand, within PP_4977 at 5670.175 kb on + strand, within PP_4977 at 5670.176 kb on - strand, within PP_4977 at 5670.176 kb on - strand, within PP_4977 at 5670.176 kb on - strand, within PP_4977 at 5670.250 kb on + strand, within PP_4977 at 5670.250 kb on + strand, within PP_4977 at 5670.250 kb on + strand, within PP_4977 at 5670.349 kb on + strand at 5670.349 kb on + strand at 5670.349 kb on + strand at 5670.349 kb on + strand at 5670.350 kb on - strand at 5670.350 kb on - strand at 5670.350 kb on - strand at 5670.350 kb on - strand at 5670.434 kb on - strand at 5670.444 kb on + strand at 5670.445 kb on - strand at 5670.458 kb on + strand at 5670.459 kb on - strand at 5670.459 kb on - strand at 5670.464 kb on - strand at 5670.539 kb on + strand at 5670.539 kb on + strand at 5670.539 kb on + strand at 5670.539 kb on + strand at 5670.539 kb on + strand at 5670.540 kb on - strand at 5670.540 kb on - strand at 5670.540 kb on - strand at 5670.542 kb on - strand at 5670.542 kb on - strand at 5670.542 kb on - strand at 5670.542 kb on - strand at 5670.565 kb on + strand at 5670.566 kb on - strand at 5670.566 kb on - strand at 5670.670 kb on + strand, within PP_4978 at 5670.671 kb on - strand, within PP_4978 at 5670.780 kb on - strand, within PP_4978 at 5670.780 kb on - strand, within PP_4978 at 5670.928 kb on + strand, within PP_4978 at 5670.928 kb on + strand, within PP_4978 at 5671.067 kb on - strand, within PP_4978 at 5671.088 kb on - strand, within PP_4978 at 5671.168 kb on + strand, within PP_4978 at 5671.168 kb on + strand, within PP_4978 at 5671.315 kb on + strand, within PP_4978 at 5671.315 kb on + strand, within PP_4978 at 5671.315 kb on + strand, within PP_4978 at 5671.316 kb on - strand, within PP_4978 at 5671.316 kb on - strand, within PP_4978 at 5671.375 kb on + strand, within PP_4978 at 5671.375 kb on + strand, within PP_4978 at 5671.375 kb on + strand, within PP_4978 at 5671.376 kb on - strand, within PP_4978 at 5671.376 kb on - strand, within PP_4978 at 5671.384 kb on + strand, within PP_4978
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,669,397 + -0.2 5,669,398 - +0.4 5,669,398 - +0.4 5,669,398 - +1.1 5,669,398 - +0.2 5,669,437 + -0.0 5,669,437 + -1.0 5,669,438 - +2.7 5,669,438 - +1.1 5,669,521 + +0.1 5,669,521 + -0.6 5,669,521 + -0.6 5,669,662 + PP_4977 0.16 -0.9 5,669,662 + PP_4977 0.16 -0.0 5,669,662 + PP_4977 0.16 -1.0 5,669,662 + PP_4977 0.16 -0.9 5,669,662 + PP_4977 0.16 -1.8 5,669,663 - PP_4977 0.16 +1.1 5,669,663 - PP_4977 0.16 -2.1 5,669,663 - PP_4977 0.16 -1.7 5,669,663 - PP_4977 0.16 +0.1 5,669,663 - PP_4977 0.16 -1.4 5,669,663 - PP_4977 0.16 +2.7 5,669,663 - PP_4977 0.16 -1.3 5,669,664 + PP_4977 0.16 +0.0 5,669,689 + PP_4977 0.19 -1.6 5,669,689 + PP_4977 0.19 -1.4 5,669,690 - PP_4977 0.19 +0.7 5,669,690 - PP_4977 0.19 -2.3 5,669,759 + PP_4977 0.26 +1.1 5,669,759 + PP_4977 0.26 -1.6 5,669,759 + PP_4977 0.26 +0.5 5,669,760 - PP_4977 0.27 +1.1 5,669,828 - PP_4977 0.34 -1.6 5,669,911 + PP_4977 0.44 -0.6 5,669,911 + PP_4977 0.44 +0.7 5,669,912 - PP_4977 0.44 +0.3 5,669,912 - PP_4977 0.44 -0.9 5,669,912 - PP_4977 0.44 -1.0 5,670,076 + PP_4977 0.62 -0.2 5,670,077 - PP_4977 0.62 +0.2 5,670,077 - PP_4977 0.62 -0.6 5,670,097 + PP_4977 0.65 +0.5 5,670,098 - PP_4977 0.65 -0.6 5,670,098 - PP_4977 0.65 -2.6 5,670,098 - PP_4977 0.65 +0.0 5,670,098 - PP_4977 0.65 -1.0 5,670,103 + PP_4977 0.65 +2.7 5,670,103 + PP_4977 0.65 +0.2 5,670,175 + PP_4977 0.73 -1.1 5,670,175 + PP_4977 0.73 -0.8 5,670,176 - PP_4977 0.73 -2.0 5,670,176 - PP_4977 0.73 +0.2 5,670,176 - PP_4977 0.73 -1.3 5,670,250 + PP_4977 0.82 -0.0 5,670,250 + PP_4977 0.82 -1.9 5,670,250 + PP_4977 0.82 +1.1 5,670,349 + +0.4 5,670,349 + -0.3 5,670,349 + -2.4 5,670,349 + -0.8 5,670,350 - -1.6 5,670,350 - +0.1 5,670,350 - -2.3 5,670,350 - -1.5 5,670,434 - +0.0 5,670,444 + +2.1 5,670,445 - +1.2 5,670,458 + -1.1 5,670,459 - +0.8 5,670,459 - +0.7 5,670,464 - +3.7 5,670,539 + -0.6 5,670,539 + +1.7 5,670,539 + -0.1 5,670,539 + -0.2 5,670,539 + +1.1 5,670,540 - +0.9 5,670,540 - +0.1 5,670,540 - -1.0 5,670,542 - +1.1 5,670,542 - +1.7 5,670,542 - +0.2 5,670,542 - -0.3 5,670,565 + +0.6 5,670,566 - -0.1 5,670,566 - -0.2 5,670,670 + PP_4978 0.16 +0.3 5,670,671 - PP_4978 0.16 -1.2 5,670,780 - PP_4978 0.26 +0.5 5,670,780 - PP_4978 0.26 +0.5 5,670,928 + PP_4978 0.40 -0.7 5,670,928 + PP_4978 0.40 +1.4 5,671,067 - PP_4978 0.53 -1.6 5,671,088 - PP_4978 0.55 -0.9 5,671,168 + PP_4978 0.62 -0.8 5,671,168 + PP_4978 0.62 -0.5 5,671,315 + PP_4978 0.75 -0.3 5,671,315 + PP_4978 0.75 -1.0 5,671,315 + PP_4978 0.75 -0.7 5,671,316 - PP_4978 0.76 -0.7 5,671,316 - PP_4978 0.76 +0.1 5,671,375 + PP_4978 0.81 +0.5 5,671,375 + PP_4978 0.81 -0.5 5,671,375 + PP_4978 0.81 -0.6 5,671,376 - PP_4978 0.81 -1.2 5,671,376 - PP_4978 0.81 -1.0 5,671,384 + PP_4978 0.82 +1.1
Or see this region's nucleotide sequence